Journal of the korean academy of Pediatric Dentistry (대한소아치과학회지)

Korean Academy of Pediatric Dentistry (대한소아치과학회)
권호 표지

THE ANTIMICROBIAL EFFECT OF HORSERADISH(ARMORACIA RUSTICANA) ROOT EXTRACTS AGAINST ANAEROBES ISOLATED FROM ORAL CAVITY

구강에서 채취한 혐기성 세균에 대한서양산 고추냉이 뿌리 추출물의 항균효과

  • Jang, Yong-Gul (Department of Pediatric Dentistry, Oral Science Research Center, College of Dentistry) ;
  • Park, Ho-Won (Department of Pediatric Dentistry, Oral Science Research Center, College of Dentistry) ;
  • Shin, Il-Sik (Faculty of Marine Food Science and Technology, Gangneung-Wonju National University) ;
  • Lee, Ju-Hyun (Department of Pediatric Dentistry, Oral Science Research Center, College of Dentistry) ;
  • Seo, Hyun-Woo (Department of Pediatric Dentistry, Oral Science Research Center, College of Dentistry)
  • 장용걸 (강릉원주대학교 치과대학 소아치과학교실 및 구강과학연구소) ;
  • 박호원 (강릉원주대학교 치과대학 소아치과학교실 및 구강과학연구소) ;
  • 신일식 (강릉원주대학교 해양생명공학부 식품미생물학교실) ;
  • 이주현 (강릉원주대학교 치과대학 소아치과학교실 및 구강과학연구소) ;
  • 서현우 (강릉원주대학교 치과대학 소아치과학교실 및 구강과학연구소)
  • Received : 2009.12.21
  • Accepted : 2010.03.11
  • Published : 2010.05.31
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Abstract

Objective : The aim of this study was to determine the antimicrobical effect of horseradish root extracts against Enterococcus faecalis and Fusobacterium nucleatum isolated from oral cavity compared with reference strain, and compared with that of chlorhexidine. Method : Horseradish root extracts and chlorhexidine were sequentially diluted and tested against anaerobes(E. faecalis, F. nucleatum) isolated from children's oral cavity. The microbes were anaerobically incubated and the minimum inhibitory concentration(MIC) and minimum bactericidal concentration(MBC) were detected. Results :1. Horseradish root extracts showed antimicrobial effect against E. faecalis isolated strain at same or slightly higher concentration compared with MIC of reference strain. 2. $625.0\sim1,250.0{\mu}g/ml$ horseradish root extracts showed similar antimicrobial effect with chlorhexidine($7.8\sim15.6{\mu}g/ml$). 3. Horseradish root extracts showed antimicrobial effect against F. nucleatum isolated strain at same or slightly higher concentration compared with MIC of reference strain. 4. $78.1\sim312.5{\mu}g/ml$ horseradish root extracts showed similar antimicrobial effect with chlorhexidine($7.8\sim15.6{\mu}g/ml$). Conclusions : The results of this study confirm that horseradish root extracts has antimicrobial effect against anaerobes isolated from oral cavity as well as reference strain. And we found the potential of horseradish root extracts as a canal irrigant or disinfectant.

서양산 고추냉이(Horseradish, Armoracia rusticana)의 주성분인 allylisothiocyanate(AIT)는 각종 세균에 대한 살균 효과로 주목받고 있다. 이전 연구들을 통해 구강내 다양한 세균 및 진균류에 대한 항균성이 입증되었으나 근단내 병소를 유발 하는 혐기성 세균에 대한 연구는 미진한 실정이다. 본 연구에서는 사람의 근단 병소에서 직접 분리한 E. faecalis와 F. nucleatum에 대한 서양산 고추냉이 뿌리 추출물의 항균활성을 표준균주와 비교해보고 이를 대표적인 항균제인 클로르헥시딘과 비교하여 다음과 같은 결론을 얻었다. 1. 서양산 고추냉이 뿌리 추출물은 E. faecalis 임상 분리균주에 대하여 표준균주와 같거나 약간 높은 농도에서 항균효과를 나타냈고, $625.0\sim1,250.0{\mu}g/ml$의 농도에서 클로르헥시딘($7.8\sim15.6{\mu}g/ml$)과 대등한 항균효과를 가지는 것으로 나타났다. 2. 서양산 고추냉이 뿌리 추출물은 F. nucleatum 임상 분리균주에 대하여 표준균주와 같거나 약간 높은 농도에서 항균효과를 나타냈고, $78.1\sim312.5{\mu}g/ml$의 농도에서 클로르헥시딘($7.8\sim15.6{\mu}g/ml$)과 대등한 항균효과를 가지는 것으로 나타났다. 이번 연구로 표준균주 뿐만 아니라 구강에서 직접 분리한 혐기성 세균에 대한 서양산 고추냉이 뿌리 추출물의 항균성을 확인할 수 있었고, 추후 근관 세척제나 소독제로 서양산 고추냉이 뿌리 추출물을 이용할 수 있는 가능성을 보여주었다.

Keywords

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