DOI QR코드

DOI QR Code

수종(數種)의 한약재(韓藥材) 추출물(抽出物)의 항산화능(抗酸化能)과 혈전용해능(血栓溶解能)

Antioxidative and Fibrinolytic Activities of Several Medicinal Plant Extracts

  • 주은영 (대구한의대학교 한자원학과) ;
  • 박찬성 (대구한의대학교 한방식품조리영양학부)
  • Joo, Eun-Young (Dept. of Herbal Biotechnology, Daegu Haany University) ;
  • Park, Chan-Sung (Faculty of Herbal Food Cuisine & Nutrition, Daegu Haany University)
  • 투고 : 2010.08.06
  • 심사 : 2010.09.13
  • 발행 : 2010.09.30

초록

Objectives : This study was conducted to evaluate the antioxidative and fibrinolytic activity of the water and ethanol extracts from medicinal plants. Methods : Five kinds of medicinal plants(Carthami Flos, Glycyrrhizae Radix, Schisandrae Fructus, Atractylodes Rhizoma, Shiitake mushiroom) were extracted with distilled water and 70% ethanol, and the extracts were tested for their antioxidative and fibrilytic activities. Results : The highest polyphenol contents of the water and ethanol extracts from medicinal plants were 812.52 mg and 685.44 mg per 100 g of Carthamus tinctorius and Schizandra chinensis, respectively. The electron donating abilities (EDA) of the water extracts from all medicinal plants except Lentinus edodes were about 90% at 1,000 ppm and ethanol extracts were higher than those of water extracts. The highest SOD-like activity and nitrite scavenging abilities (NSA) were both of water and ethanol extracts from Schizandra chinensis. Five kinds of medicinal plants had fibrinolytoc activity and the highest activities were water and ethanol extracts from Glycyrrhiza uralensis. Conclusion : These results suggest that the medicinal plants can be used as natural antioxidant to prevent oxidative damage in normal cells probably because of their antioxidative and fibrinolytic activities.

키워드

참고문헌

  1. 통계청(2009) : 사망원인통계(전국편, 2008년) http://www.kosis.kr/nsportal/ups/ups_01List01.jsp? pubcode=YD
  2. Kwon SJ, Lim CY, Kim JS, Park MH, Lee SY. Fibrinolytic activities and effects of gamma-irradiated on seeds from Coix Lacryma-jobi L., Carthamus tinctorius L. and Malva verticillata L. Korean J Biotechnol Bioeng. 2006;21(1):20-27.
  3. Marks D, Marks A, Smith C. Basic medical biochemistry. Baltimore, Wilkins & Wilkins. 1996:107.
  4. Kim SH, Choi NS, Lee WY, Lee JW and Kim DH Isolation of Bacillus strains secreting fibrinolytic enzymes from Doen-Jang. Korean J Microbiol. 1998;34(2):87-90.
  5. Finkel T, Holbrook NJ. Oxidants, oxidative stress and the biology of ageing. Nature, 2002;408: 239-247.
  6. Martindale JL, Holbrook NJ. Cellular response to oxidative stress: signaling for suicide and survival. J Cell Physiol. 2002;192:1-15. https://doi.org/10.1002/jcp.10119
  7. Ji LL. Antioxidant enzyme response to exercise and aging. Med Sci Sport Exercise. 1993;25(2):225-231.
  8. Seo CJ, Yi SM, Ko YW. The effect of antioxidant supplement on the activity of SOD, CAT and MDA in high intensity aerobic exercise. J Kor Sport Res. 2007;18(2):21-31. https://doi.org/10.24985/kjss.2007.18.4.21
  9. Burton GW, Taber MG. Vitamin E : antioxidant activity, biokinetics, and bioavailability. Annu Rev. 1990;10:357-382. https://doi.org/10.1146/annurev.nu.10.070190.002041
  10. Kim DH, Kang YG, Kim H, Chae HJ. Investigation of antidiabetic medicinal plants using an oriental medicinal database. Korean J Biotechnol Bioeng. 2004;19(2):125-131.
  11. Park KH, Kim SY, Chae HJ. Selection of oriental medicinal plants for screening of anticancer agents. Korean J Biotechnol Bioeng. 2007;22(3): 139-145.
  12. Kim JH, Park JH, Park SD, Choi SY, Seong JH, Moon KD. Preparation and antioxidant activity of health drink with extract powders from safflower (Carthamus tinctorius L.) seed. Korean J Food Sci Technol. 2002;34(4):617-624.
  13. Kim KW, Suh SJ, Lee TK, Ha KT, Kim JK, Kim KH, Kim DI, Jeon JH, Moon TC, Kim CH. Effect of safflower seeds supplementation on stimulation of the proliferation, differentiation and mineralization of osteoblastic MC3T3-E1 cells. J Ethnopharmacology. 2008;115(1) 42-49. https://doi.org/10.1016/j.jep.2007.09.003
  14. Seo BI, Lee ES, Park JH, Kim SC, Byun BH, Choi HY. A efficacy of Korean and Chinese Carthami Semen on analgesic effect, anti - inflammation and arthritis. Kor. J Herbology. 2001;16(1):1-10.
  15. 서부일, 변부형, 신순식, 김봉현. 한방식품학. 대구:벧엘기획. 2003;265-267.
  16. Kim CH, Jeong JG. Antioxidant activities and the effect of reducing serum alcohol concentration of Lentinus edodes. Kor. J Herbology. 2009;24(4): 159-164.
  17. Chang JH, Kim MS, Kim JY, Choi WH, Lee SS. Effects of mushroom supplementation on blood glucose concentration, lipid profile, and antioxidant enzyme activities in patients with type 2 diabetes mellitus. Korean J Nutr. 2007;40(4): 327-333.
  18. Park JM, Lee SH, Kim JO, Park HJ, Park JB, Sin JI. In vitro and in vivo effects of extracts of Lentinus edodes on tumor growth in a human Papillomavirus 16 Oncogenes-transformed animal tumor model - Apoptosis-mediated tumor cell growth inhibition. Korean J Food Sci Technol. 2004;36(1):141-146.
  19. Yoon TS, Cheon MS, Kim SJ, Lee AY, Moon BC, Chun JM, Choo BK, Kim HK. Evaluation of solvent extraction on the anti-inflammatory efficacy of Glycyrrhiza uralensis . Korean J Medicinal Crop Sci. 2010;18(1):28-33.
  20. Kim JS, Choi SY. Quality characteristics of soybean curd with Omija extract. Korean J Food & Nutr. 2008;21(1):43-50.
  21. Choi SY, Sung NJ, Kim HJ. Physicochemical characteristics of traditional Doenjang with added Lentinus edodes. Korean J Food Cookery Sci. 2006;22(1):69-79.
  22. Kim ML, Park EJ, Jeong JS. Sensory characteristics of Doenjang with added Licorice powder as assessed by response surface methodology. Korean J Food Cookery Sci. 2010;26(1):62-71
  23. AOAC. Official method of analysis. 15th ed. Association of official analytical chemists, Washington D.C. USA. 1990.
  24. Blois MS. Antioxidant determination by the use of a stable free radical. Nature. 1958;26:1198.
  25. Marklund S, Marklund G. Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur. J. Biochem. 1974;47:469-474. https://doi.org/10.1111/j.1432-1033.1974.tb03714.x
  26. Kato H, Chuyen NV, Kim SB, Hayase F. Inhibition of nitrosamine formmation by nondialyzable melanoidins. Agric. Biol. Chem. 1987;51:1333. https://doi.org/10.1271/bbb1961.51.1333
  27. Astrup TS, Mullertz S. The fibrin plate method for estimating fibrinolytic activity. Arch Biochim Biophys. 1952;40:346-351. https://doi.org/10.1016/0003-9861(52)90121-5
  28. Lee DH. A study on SOD activity and serum antioxidant mineral concentrations in obese adolescents. Kor J Nutr. 2007;40(1):41-48.
  29. Yoon MY, Lee BB, Kim JY, Kim YS, Park EJ, Lee SC, Park HR. Antioxidant activity and neuroprotective effect of Psoralea corylifolia Linne extracts. Kor J Pharmacogn. 2007;38(1):84-89.
  30. Kim SS, Lee JH, An YS, Kim JH, Kang DG. A fibrinolytic enzyme from Bacillus amyloliquefaciens D4-7 isolated from Chungkook-Jang; It's characterization and influence of additives on thermostability. Korean J Microbiol Biotechnol. 2003;31(3):271-276.
  31. Kim SI, Sim KH, Ju SY, Han YS. A Study on antioxidative and hypoglycemic activities of Omija(Schizandra chinensis Baillon) extract under variable extract conditions. Korean J Food & Nutr. 2009;22(1):41-47.
  32. Kim SJ, Kweon DH, Lee JH. Investigation of antioxidative activity and stability of ethanol extracts of Licorice root (Glycyrrhiza glabra). Korean J Food Sci Technol. 2006;38(4):584-588.
  33. Rho SN, Oh HS. Effect of Omija(Schizandra Chinensis Baillon) extracts on the growth of liver cancer cell line SNU - 398. Korean J Nutr. 2002;35(2):201-206
  34. Lee SO, Seo JH, Lee JW, Yoo MY, Kwon JW, Choi SU, Kang JS, Kwon DY, Kim YK, Kim YS, Ryu SY. Inhibitory effects of the Rhizome extract of Atractylodes japonica on the proliferation of human tumor cell lines. Korean J Pharmacogn. 2005;36(3):201-204.
  35. Kimura Y, Iijima S, Kato T, Tsujie M, Naoi Y, Hayashi T, Tanigawa T, Yamamoto H, Kurokawa E, Kikkawa N, Matsumura N. Usefulness of TS-1 and lentinan combination immunuchemotherapy in advanced or recurrent gastric cancer-pilot study aiming at a randomized trial. Gan To Kagaku Ryoho. 2003;30:1125-1130.