Preventive Nutrition and Food Science

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Codium fragile Ethanol Extraction Inhibited Inflammatory Response through the Inhibition of JNK Phosphorylation

  • Han, Sin-Hee (Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science, RDA) ;
  • Kim, Young-Guk (Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science, RDA) ;
  • Lee, Su-Hwan (Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science, RDA) ;
  • Park, Chung-Berm (Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science, RDA) ;
  • Choi, Han-Gil (Faculty of Biological Science and Institute of Biotechnology, Wonkwang University) ;
  • Jang, Hye-Jin (Department of Oriental Pharmacy, College of Pharmacy, Wonkwang University, Wonkwang Oriental Medicines Research Institute) ;
  • Lee, Young-Seob (Department of Oriental Pharmacy, College of Pharmacy, Wonkwang University, Wonkwang Oriental Medicines Research Institute) ;
  • Kwon, Dong-Yeul (Department of Oriental Pharmacy, College of Pharmacy, Wonkwang University, Wonkwang Oriental Medicines Research Institute)
  • Received : 2010.06.25
  • Accepted : 2010.08.11
  • Published : 2010.09.30
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Abstract

Codium fragile (CF) is an edible green alga consumed as a traditional food source in Korea. In this study, the ethanol extract of CF was evaluated to determine if it has anti-inflammatory activity. Lipopolysaccharide (LPS), a toxin from bacteria, is a potent inducer of inflammatory cytokines, such as tumor necrosis factor (TNF)-$\alpha$ and interleukin (IL)-6. Therefore, we studied whether CF extracts have an anti-inflammatory effect in LPS-induced murine macrophage cell lines (RAW 264.7). In the present study, IL-6 production was measured using an enzyme-linked immunosorbent assay (ELISA), prostaglandin $E_2$($PGE_2$) production was measured using the EIA kit, and cyclooxygenase (COX)-2 and mitogen-activated protein kinase (MAPK) activation were determined by Western blot analysis. IL-6 mRNA, COX-2 mRNA and iNOS mRNA expression were measured using reverse transcription-polymerase chain reaction (RT-PCR). The results indicated that CF extracts inhibit LPS-induced IL-6, NO and PGE2 production in a dose-dependent manner, as well as expression of iNOS and COX-2. CF extracts significantly inhibited LPS-induced c-Jun N-terminal kinase (JNK) 1/2 phosphorylation. Taken together, these findings may help elucidate the mechanism by which CF modulates RAW 264.7 cell activation under inflammatory conditions.

Keywords

References

  1. Medzhitov R, Janeway CA Jr. 1998. An ancient systemof host defense. Curr Opin Immuno 10: 12-15. https://doi.org/10.1016/S0952-7915(98)80024-1
  2. Hoffmann JA, Kafatos FC, Janeway CA, Ezekowitz RA.1999. Phylogenetic perspectives in innate immunity. Science284: 1313-1318. https://doi.org/10.1126/science.284.5418.1313
  3. Dziarski R, Ulmer AJ, Gupta D. 2000. Interactions ofCD14 with components of Gram-positive bacteria. ChemImmunol 74: 83-107.
  4. Schleifer KH, Kandler O. 1972. Bacteriol Rev 36: 407-477.
  5. Wang Q, Dziarski R, Kirschning CJ, Muzio M, Gupta D.2001. Infect Immun 69: 2270-2276. https://doi.org/10.1128/IAI.69.4.2270-2276.2001
  6. Xu Z, Dziarski R, Wang Q, Swartz K, Sakamoto KM,Gupta D. 2001. J Immunol 167: 6975-6982 https://doi.org/10.4049/jimmunol.167.12.6975
  7. Bhakdi S, Klonisch T, Nuber P, Fischer W. 1991. InfectImmun 59: 4614-4620.
  8. Mattsson E, Verhage L, Rollof J, Fleer A, Verhoef J, vanDijk H. 1993. Peptidoglycan and teichoic acid fromStaphylococcus epidermidis stimulate human monocytesto release tumour necrosis factor, interleukin-1 ${\beta}$ and interleukin-6. FEMS Immunol Med Microbiol 7: 281-287.
  9. Pierce GF. 1990. Macrophages: important physiologic andpathologic sources of polypeptide growth factors. Am JRespir Cell Mol Biol 2: 233-234. https://doi.org/10.1165/ajrcmb/2.3.233
  10. Simons RK, Junger WG, Loomis WH, Hoyt DB. 1996.Acute lung injury in endotoxemic rats is associated withsustained circulating IL-6 levels and intrapulmonary CINCactivity and neutrophil recruitment role of circulatingTNF-alpha and IL-beta? Shock 6: 39-45. https://doi.org/10.1097/00024382-199607000-00009
  11. Guslandi M. 1998. Nitric oxide and inflammatory boweldisease. Eur J Clin Invest 28: 904-907. https://doi.org/10.1046/j.1365-2362.1998.00377.x
  12. Ritchlin CT, Haas-Smith SA, Li P, Hicks DG, Schwarz EM.2003. Mechanisms of $TNF-{\alpha}$ and RANKL-mediated osteoclastogenesisand bone resorption in psoriatic arthritis.J Clin Invest 111: 821-831. https://doi.org/10.1172/JCI200316069
  13. Chen C, Chen YH, Lin WW. 1999. Involvement of p38mitogen-activated protein kinase in lipopolysaccharide-inducediNOS and COX-2 expression in J774 macrophage.Immunology 97: 124-129. https://doi.org/10.1046/j.1365-2567.1999.00747.x
  14. Chan ED, Riches DW. 1998. Potential role of the JNK/SAPK signal transduction pathway in the induction ofiNOS by $TNF-{\alpha}$ Biochem Biophys Res Commun 253: 790-796. https://doi.org/10.1006/bbrc.1998.9857
  15. Kim YH, Lee SH, Lee JY, Choi SW, Park JW, Kwon TK.2004. Triptolide inhibits murine inducible nitric oxide synthaseexpression by down-regulating lipopolysaccharideinducedactivity of nuclear factor-kappa B and c-Jun$NH_2-terminal$ kinase. Eur J Pharmacol 494: 1-9. https://doi.org/10.1016/j.ejphar.2004.04.040
  16. Zhu W, Chiu LCM, Ooi VEC, Chan PKS, Ang PO. 2006.Antiviral property and mechanisms of a sulphated polysaccharidefrom the brown alga Sargassum patens againstHerpes simplex virus type 1. Phytomedicine 13: 695-701. https://doi.org/10.1016/j.phymed.2005.11.003
  17. Oh KB, Lee JH, Chung SC, Shin J, Shin HJ, Kim HK,Lee HS. 2008. Antimicrobial activities of the bromophenolsfrom the red alga Odonthalia corymbifera and somesynthetic derivatives. Bioorg Med Chem Lett 18: 104- 108. https://doi.org/10.1016/j.bmcl.2007.11.003
  18. Bennamara A, Abourriche A, Berrada M, Charrouf M,Chaib N, Boudouma M, Garneau FX. 1999. Methoxybifurcarenone:An antifungal and antibacterial meroditerpenoidfrom the brown alga Cystoseira tamariscifolia.Phytochemistry 52: 37-40. https://doi.org/10.1016/S0031-9422(99)00040-0
  19. Glombitza KW, Koch M. 1989. Secondary metabolites ofpharmaceutical potential. In Algal and CyanobacterialBiotechnology. Cresswell RC, Rees TAV, Shah N, eds.Longman Scientific & Technical, Harlow, Essex, England.p 161-238.
  20. Kwon HJ, Bae SY, Kim KH, Han CH, Cho SH, Nam SW,Choi YH, Kim BW. 2007. Induction of apoptosis in HeLacells by ethanolic extract of Corallina pilulifera. FoodChem 104: 196-201. https://doi.org/10.1016/j.foodchem.2006.11.031
  21. Cavas L, Baskin Y, Yurdakoc K, Olgun N. 2006. Antiproliferativeand newly attributed apoptotic activities from aninvasive marine alga: Caulerpa racemosa var. cylindracea.J Exp Mar Biol Ecol 339: 111-119. https://doi.org/10.1016/j.jembe.2006.07.019
  22. Athukorala Y, Kim KN, Jeon YJ. 2006. Antiproliferativeand antioxidant properties of an enzymatic hydrolysatefrom brown alga, Ecklonia cava. Food Chem Toxicol 44:1065-1074. https://doi.org/10.1016/j.fct.2006.01.011
  23. Zhou G, Xin H, Sheng W, Sun Y, Li Z, Xu Z. 2005. Invivo growth inhibition of S180 tumor by mixture of 5-Fuand low molecular ${\lamda}-carrageenan$ from Chondrus ocellatus.Pharmacol Res 51: 153-157. https://doi.org/10.1016/j.phrs.2004.07.003
  24. Zhang YS. 1994. Dictionary of Chinese marine drugs.China Ocean Press, Beijing, China. p 507.
  25. MacMicking J, Xie QW, Nathan C. 1997. Nitric oxide andmacrophage function. Annu Rev Immunol 15: 323-350. https://doi.org/10.1146/annurev.immunol.15.1.323
  26. Ohshima H, Bartsch H. 1994. Chronic infections and inflammatoryprocesses as cancer risk factors: possible roleof nitric oxide in carcinogenesis. Mutat Res 305: 253-264. https://doi.org/10.1016/0027-5107(94)90245-3
  27. Szabo C. 1995. Alterations in nitric oxide production invarious forms of circulatory shock. New Horiz 3: 2-32.
  28. Koo TH, Lee JH, Park YJ, Hong YS, Kim HS, Kim KW,Lee JJ. 2001. A sesquiterpene lactone, costunolide, fromMagnolia grandiflora inhibits NF-kappa B by targetingI kappa B phosphorylation. Planta Med 67: 103-107. https://doi.org/10.1055/s-2001-11503
  29. Johnson GL, Lapadat R. 2002. Mitogen-activated proteinkinase pathways mediated by ERK, JNK, and p38 proteinkinases. Science 298: 1911-1912. https://doi.org/10.1126/science.1072682
  30. Kang OH, Lee GH, Choi HJ, Park PS, Chae HS, JeongSI, Kim YC, Sohn DH, Park H, Lee JH, Kwon DY. 2007.Ethyl acetate extract from Angelica Dahuricae Radix inhibitslipopolysaccharide-induced production of nitric oxide,prostaglandin E2 and tumor necrosis factor-alpha viamitogen-activated protein kinases and nuclear factor-kappaB in macrophages. Pharmacol Res 55: 263-270. https://doi.org/10.1016/j.phrs.2006.12.001
  31. Chen C, Chen YH, Lin WW. 1999. Involvement of p38mitogen-activated protein kinase in lipopolysaccharide- inducediNOS and COX-2 expression in J774 macrophages.Immunology 97: 124-129. https://doi.org/10.1046/j.1365-2567.1999.00747.x
  32. Kim SH, Kim J, Sharma RP. 2004. Inhibition of p38 andERK MAP kinases blocks endotoxin-induced nitric oxideproduction and differentially modulates cytokine expression.Pharmacol Res 49: 433-439. https://doi.org/10.1016/j.phrs.2003.11.004