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Analysis of Nitrate Contents of Agricultural Products by HPLC-UV

HPLC-UV를 이용한 농산물의 질산염 함량분석

  • Jo, Sung-Ae (Kangnam Agricultural Products Inspection Team, Kangnam Agro-marin Products Inspection Center, Research Institute of Public Health & Environment) ;
  • Kim, Eun-Hee (Kangnam Agricultural Products Inspection Team, Kangnam Agro-marin Products Inspection Center, Research Institute of Public Health & Environment) ;
  • Han, Sung-Hee (Kangnam Agricultural Products Inspection Team, Kangnam Agro-marin Products Inspection Center, Research Institute of Public Health & Environment) ;
  • Yuk, Dong-Hun (Kangnam Agricultural Products Inspection Team, Kangnam Agro-marin Products Inspection Center, Research Institute of Public Health & Environment) ;
  • Kim, Jung-Hun (Kangnam Agricultural Products Inspection Team, Kangnam Agro-marin Products Inspection Center, Research Institute of Public Health & Environment) ;
  • Park, Seog-Gee (Kangnam Agricultural Products Inspection Team, Kangnam Agro-marin Products Inspection Center, Research Institute of Public Health & Environment)
  • 조성애 (서울시보건환경연구원 강남농수산물검사소 농산물검사팀) ;
  • 김은희 (서울시보건환경연구원 강남농수산물검사소 농산물검사팀) ;
  • 한성희 (서울시보건환경연구원 강남농수산물검사소 농산물검사팀) ;
  • 육동현 (서울시보건환경연구원 강남농수산물검사소 농산물검사팀) ;
  • 김정헌 (서울시보건환경연구원 강남농수산물검사소 농산물검사팀) ;
  • 박석기 (서울시보건환경연구원 강남농수산물검사소 농산물검사팀)
  • Received : 2010.06.08
  • Accepted : 2010.07.22
  • Published : 2010.09.30

Abstract

A total of 550 samples were analysed for nitrate contents using HPLC-UV. Nitrate contents of leaf vegetables were higher than those of root vegetables and fruit vegetables. The average nitrate content of the radish leaves (4875.8 mg/kg) was the highest, followed by marsh mallow (4711.7 mg/kg), crown daisy (4546.9 mg/kg) and vitamins (4239.5 mg/kg). The nitrate content in fruits of strawberry and banana averaged at 24.0 mg/kg and 438.5 mg/kg, respectively. Nitrate was not detected in other fruits. In fruiting vegetables nitrate contents were less than 1000 mg/kg. In onion, lotus root and radish, nitrate contents were 253.7mg/kg, 352.4mg/kg and 2849.0 mg/kg, respectively, with no detection in garlic. Nitrate contents in mushrooms were less than 100 mg/kg.

엽채류 19종 407건, 과실류 5종 68건, 과채류 3종 32건, 근채류 4종 34건 및 버섯류 3종 10건의 질산염 함량 분석결과를 보면 엽채류에서의 질산염 함량은 무잎이 평균 4875.8 mg/kg으로 가장 함량이 높았으며, 다음이 아욱(4711.8 mg/kg), 쑥갓(4546.9 mg/kg), 비타민(4239.5 mg/kg) 순으로 나타났다. 비타민, 상추, 그리고 깻잎의 질산염 함량은 443.3~6746.5 mg/kg, 583.2~4189.5 mg/kg, 73.0~3405.3 mg/kg으로 시료간의 개체 차이가 9~15배 정도로 큰 것으로 나타났다. 과실류에서의 질산염 함량은 딸기가 최저 12.8 mg/kg, 최고 35.2 mg/kg, 평균 24.0 mg/kg으로 나타났으며, 바나나 최저 423.6 mg/kg, 최고 453.2 mg/kg, 평균 438.5 mg/kg으로 나타났다. 딸기와 바나나를 제외하고는 다른 과실류에서는 질산염이 검출되지 않았다. 과채류에서는 종류에 따라 차이가 있으나 평균 1,000 mg/kg 이하의 낮은 함량분포를 보였다. 근채류와 버섯류에서의 질산염 함량은 마늘에서는 질산염이 검출되지 않았으며, 양파, 연근 그리고 적환의 평균 질산염 함량은 253.4 mg/kg, 352.4 mg/kg, 2849.0 mg/kg으로 나타났으며, 버섯류에서 새송이버섯과 느타리버섯의 평균 질산염 함량은 55.3 mg/kg, 98.2 mg/kg이며, 팽이버섯에서는 검출되지 않았다.

Keywords

References

  1. Walker R. 1996. The metabolism of dietary nitrites and nitrates. Bioactive Components of Food 24: 780-785.
  2. Sohn SM. 2000. The reduction technology of nitrate content in vegetable. Ministry Agriculture and Forestry Report.
  3. World Health Organization (WHO). 2004. Drinking-water guideline quality.
  4. Ysart G, Miller P, Barrett G, Farrington D, Lawrances P, Harrison N. 1999. Dietary exposures to nitrate in the UK. Food Addit Contam 16: 521-532. https://doi.org/10.1080/026520399283669
  5. The EFSA Journal. 2008. Nitrate in vegetables. p 1-79.
  6. Oh MC, Oh CK, Kim SY. 1996. Rapid analytical method of nitrate and nitrate in fish by ion chromatography. J Food Sci Nutr 1: 1-5.
  7. Kim BY, Yoon S. 2003. Analysis of nitrate contents of Korean common foods. J Korean Soc Food Sci Nutr 32: 779-784. https://doi.org/10.3746/jkfn.2003.32.6.779
  8. Lee JY, Park YH, Jang BC, Kim SC, Kim PJ, Ryu SN. 2005. Variation of nitrate contents on distributed vegetables in Korea. Korean J Crop Sci 50: 231-238.
  9. Lee EH, Kim SK, Jeon JK, Chung SH, Cha YJ. 1982. Nitrate and nitrite content of some fermented sea foods and vegetables. Bull Korean Fish Soc 15: 147-153.
  10. Walker R. 1990. A review of the occurrence in food and diet and the toxicological implications. Food Addit Contam 7: 717-768. https://doi.org/10.1080/02652039009373938
  11. Sohn SM, Kim YH, Yoon JY. 1999. Nitrate contents in vegetables cultivated by organic farming in Korea. Korean J Organic Agriculture 7: 125-151.
  12. Sohn SM, Oh KS. 1993. Influence of nitrogen level on the accumulation of NO3 - on edible parts of Chinese cabbage, radish and cucumber. J Korean Soc Soil Sci 26: 10-19.
  13. Sohn SM, Oh KS. 1993. Study on utility of nitrate content in edible parts of crops as an indicator of simplified judgment for superior agricultural products by low nitrogen input. Korean J Organic Agriculture 2: 2-15.
  14. Sohn SM, Oh KS, Lee JS. 1995. Effects of shading and nitrogen fertilization on yield and accumulation of $NO_3^-$ in edible parts of Chinese cabbage. J Korean Soc Soil Sci Fert 28: 154-159.
  15. Lee GJ, Kang BG, Kim HJ, Min KB. 2000. Effect of shading and nitrogen level on the accumulation of $NO_3^-$ in leaf of lettuce. Korean J Environ Agric 19: 294-299.
  16. Lee YJ, Chung JB. 2006. Comparison of nitrate accumulation in lettuce grown under chemical fertilizer or compost applications. Korean J Environ Agric 25: 339-345. https://doi.org/10.5338/KJEA.2006.25.4.339
  17. Choi IH, Kim SW, Nahm KH. 1996. A study on the nitrate contents in vegetables. Kor J Anim Nutr Feed 22: 49-54.
  18. Yang YJ, Lee KA, Kim KJ. 2000. Effect of pre- and postharvest on factors on nitrate contents of radish and Chinese cabbage. J Kor Soc Hort Sci 41: 365-368.

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