Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Characterization of Low-Trans Solid Fat from Canola and Fully Hydrogenated Soybean Oil by Lipase-Catalyzed Interesterification Reaction

효소적 에스테르 교환 반응 시 카놀라유와 대두극도경화유의 비율에 따른 저트랜스 고체지방의 특성

  • Kim, Young-Joo (Dept. of Food Science and Technology, Chungnam National University) ;
  • Lyu, Hyun-Kyeong (Dept. of Food Science and Technology, Chungnam National University) ;
  • Lee, Seon-Mo (Dept. of Food Science and Technology, Chungnam National University) ;
  • Lee, Ki-Teak (Dept. of Food Science and Technology, Chungnam National University)
  • Received : 2010.05.14
  • Accepted : 2010.08.04
  • Published : 2010.09.30
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Abstract

Lipase-catalyzed interesterification of canola (CO) and fully hydrogenated soybean oil (FHSBO) at different weight ratios (70:30, 75:25, and 80:20) was performed in a batch type reactor to produce low-trans solid fats. Each reaction was conducted in the shaking water bath for various reaction times (1, 3, 6, 18 and 24 hr) at 70oC and 220 rpm using Lipozyme TLIM (20 wt% of total substrate) from Thermomyces lanuginosus. After 24 hr reaction, solid fat content (SFC) by differential scanning calorimetry (DSC), fatty acid and triacylglycerol (TAG) composition of low-trans solid fats were determined. SFC of the products was reduced when the content of canola oil in the reaction mixture was increased. Major fatty acids were stearic acid (C18:0), oleic acid (C18:1) and linoleic acid (C18:2). Trans fatty acid content in the low-trans solid fats showed less than 0.3 wt%. In the HPLC analysis, major TAG species showed LOO (linoleyl-oleoyl-oleoyl), OOO, POO/SOL, SOO, and SOS.

카놀라유, 대두극도경화유를 기질로 이용하여 저트랜스 고체지방을 회분식 반응기(batch type reactor)에 의해 합성하였다. Sn-1,3 위치 특이성을 가진 TLIM을 이용한 효소적 에스테르 교환 반응을 통하여 생성된 저트랜스 고체지방의 이화학적 특성을 살펴보았다. DSC 분석을 통하여 저트랜스 고체지방의 고체지방 함량(SFC)과 융점 곡선 및 결정화 곡선을 알아보았다. 다양한 혼합비율의 저트랜스 고체지방들은 각 기질이 가진 지방산의 비율에 따라 차별적으로 넓은 범위의 온도를 나타내었다. 즉, 카놀라유의 함량이 높아질수록 각 온도에 해당하는 SFC는 감소하는 경향을 나타낸 반면, 대두극도경화유의 함량이 높아질수록 SFC가 증가하는 경향을 보였다. DSC를 이용한 흡열 및 발열 피크 분석결과, 카놀라유의 함량이 증가할수록 피크가 낮은 온도 쪽으로 이동하는 경향을 보여주었다. 저트랜스 고체지방들의 지방산 조성은 C18:0와 C18:1, C18:2가 전체 지방산 조성의 80% 이상 구성되어 있으며, 총 트랜스 지방산(${\Sigma}TFA$)의 함량은 0.3 wt% 이하의 낮은 함량을 나타내었다. Sn-2 position의 주요 지방산 또한 C18:0와 C18:1, C18:2가 대부분을 차지하였으며 특히 불포화지방산의 함량이 높게 나타났다. 역상 HPLC를 이용하여 TAG 조성을 분석한 결과, 주요 TAG 조성은 LOO, OOO, POO/SOL, SOO, SOS로 구성되어 있었다. 그중 카놀라유의 함량이 증가할수록 저트랜스 고체지방의 TAG 조성은 LOO, OOO, POO/SOL, SOO의 수치가 증가하였고, 대두극도경화유의 함량이 감소할수록 POS/PSP, SOS, SSS의 수치는 줄어드는 것을 확인할 수 있었다.

Keywords

References

  1. Jang YS. 2002. Prospect and situation of quality improvement in oilseed rape. Korean J Crop Sci 47: 175-185.
  2. Kim BH, Lumor SE, Akoh CC. 2008. Trans-free margarines prepared with canola oil/palm stearin/palm kernel oil-based structured lipids. J Agric Food Chem 56: 8195-8205. https://doi.org/10.1021/jf801412v
  3. Valsta LM, Jauhiainen M, Aro A, Katan MB, Mutanen M. 1992. Effects of a monounsaturated rapeseed oil and a polyunsaturated sunflower oil diet on lipoprotein levels in humans. Arterioscler Thromb Vasc Biol 12: 50-57. https://doi.org/10.1161/01.ATV.12.1.50
  4. Ribeiro APB, Grimaldi R, Gioielli LA, Goncalves LAG. 2009. Zero trans fats from soybean oil and fully hydrogenated soybean oil: physico-chemical properties and food applications. Food Res Int 42: 401-410. https://doi.org/10.1016/j.foodres.2009.01.012
  5. Cha IS, Park KM. 1997. Effect of catalyst on the hydrogenation of rapeseed oil. J Korean Food Sci Technol 29: 687-692.
  6. Noh KH, Won MS, Song YS. 2003. Trans fatty acid isomers of processed foods commonly consumed in Korea. J Korean Soc Food Sci Nutr 32: 325-337. https://doi.org/10.3746/jkfn.2003.32.3.325
  7. Khosla P, Hayes KC. 1996. Dietary trans-monounsaturated fatty acids negatively impact plasma lipids in humans: critical review of the evidence. J Am Coll Nutr 15: 325-339. https://doi.org/10.1080/07315724.1996.10718607
  8. Gil BI, Rho JH. 2007. Hazardous effect of dietary trans fats on human health and regulations. Korean J Soc Food Cookery Sci 23: 1015-1024.
  9. Lee JH, Akoh CC, Lee KT. 2007. Physicochemical and volatiles characterization of trans-free solid fats produced by lipase-catalyzed interesterification. J Food Sci 72: 368-374. https://doi.org/10.1111/j.1750-3841.2007.00412.x
  10. Adhikari P, Shin JA, Lee JH, Hu JN, Hwang KT, Lee KT. 2009. Enzymatic production of trans-free hard fat stock from fractionated rice bran oil, fully hydrogenated soybean oil, and conjugated linoleic acid. J Food Sci 74: 87-96. https://doi.org/10.1111/j.1750-3841.2009.01294.x
  11. Cho EJ, Lee JH, Lee KT. 2004. Optimization of enzymatic synthesis condition of structured lipids by response surface methodology. Korean J Food Sci Technol 36: 531-536.
  12. Kim JY, Lee KT. 2009. Characterization of scaled-up lowtrans shortening from rice bran oil and high oleic sunflower seed oil with batch type reactor. J Korean Soc Food Sci Nutr 38: 338-345. https://doi.org/10.3746/jkfn.2009.38.3.338
  13. Jeon MS, Lee YJ, Kang JH, Lee JH, Lee KT. 2009. Modification of palm mid fraction with stearic acid by enzymatic acidolysis reaction. J Korean Soc Food Sci Nutr 38: 479-485. https://doi.org/10.3746/jkfn.2009.38.4.479
  14. Akoh CC, Min DB. 2002. Food lipids. In Structured Lipids.Akoh CC, ed. Marcel Dekker Inc. New York, USA. p 877-908
  15. Lee KT, Akoh CC. 1998. Structured lipids: synthesis and applications. Food Rev Int 14: 17-34. https://doi.org/10.1080/87559129809541148
  16. KFDA. 2009. Department of food standardization, labeling of trans fats. Korea Food & Drug Administration, Seoul, Korea. p 41327.
  17. Fomuso LB, Akoh CC. 2002. Lipase-catalyzed acidolysis of olive oil and caprylic acid in a bench-scale packed bed bioreactor. Food Res Int 35: 15-21. https://doi.org/10.1016/S0963-9969(00)00158-7
  18. Osorio NM, da Fonseca MMR, Ferreira-Dias S. 2006. Operational stability of Thermomyces lanuginosa lipase during interesterification of fat in continuous packed-bed reactors. Eur J Lipid Sci Technol 108: 545-553. https://doi.org/10.1002/ejlt.200600029
  19. Criado M, Hernandez-Martin E, Lopez-Hernandez A, Otero C. 2007. Enzymatic interesterification of extra virgin olive oil with a fully hydrogenated fat: characterization of the reaction and its products. J Am Oil Chem Soc 84: 717-726. https://doi.org/10.1007/s11746-007-1104-y
  20. Lida HMDN, Ali ARM. 1998. Physicochemical characteristics of palm-based oil blends for the production of reduced fat spreads. J Am Oil Chem Soc 75: 1625-1631. https://doi.org/10.1007/s11746-998-0103-y
  21. Freeman IP. 1968. Interesterification. I. Change of glyceride composition during the course of interesterification. J Am Oil Chem Soc 45: 456-460. https://doi.org/10.1007/BF02655508