The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
권호 표지
DOI QR코드

DOI QR Code

First Report of Cucumber mosaic virus Infecting Pinewood Coneflower (Rudbeckia bicolor) in Korea

  • Kim, Mi-Kyeong (Agricultural Microbiology Division, National Academy of Agricultural Science) ;
  • Kwak, Hae-Ryun (Agricultural Microbiology Division, National Academy of Agricultural Science) ;
  • Ko, Sug-Ju (Jeonnam Agricultural Research and Extension Services) ;
  • Lee, Su-Heon (Agricultural Microbiology Division, National Academy of Agricultural Science) ;
  • Kim, Jeong-Soo (Agricultural Microbiology Division, National Academy of Agricultural Science) ;
  • Kim, Kook-Hyung (Department of Agricultural Biotechnology and Plant Genomics and Breeding Institute, Seoul National University) ;
  • Cha, Byeong-Jin (Department of Plant Medicine, Chungbuk National University) ;
  • Choi, Hong-Soo (Agricultural Microbiology Division, National Academy of Agricultural Science)
  • Received : 2009.12.21
  • Accepted : 2010.02.15
  • Published : 2010.03.01
Download PDF
⟨ Previous Next ⟩

Abstract

A virus isolate causing symptoms of yellow mosaic, fern leaves, malformation and plant necrosis on Rudbeckia bicolor was prevalent around Pyeongchang area in Korea. The causal virus was identified as Cucumber mosaic virus (CMV) using characteristics from biological, serological and molecular analyses and named as CMV-Rb. CMV-Rb caused mosaic on Nicotiana benthamiana, N. tabacum, Capsicum annuum, and Lycopersicon esculentum. However, typical local lesions did not develop on inoculated Pisum sativum, Cucurbita moschata, Datura stramonium and Tetragonia expansa plants. Full-length genome sequences of CMV-Rb RNAs 1, 2 and 3 were obtained using 12 primer pairs by RT-PCR analysis. The genome of CMV-Rb RNA segments 1, 2, and 3 consists of 3363nt, 3049nt, and 2214nt in length, respectively. In order to ascertain their taxonomic identity, nucleotide and the deduced amino acid sequence analyses RNAs 1, 2 and 3 of CMV-Rb isolates were conducted with previously reported sequences of CMV strains and/or isolates. CMV-Rb RNAs showed about 90 to 99% sequence identity to those of subgroup I strains suggesting that CMV-Rb is more closely related to CMV isolates belong to subgroup I. To our knowledge, this is the first report of CMV on Rudbeckia bicolor in Korea.

Keywords

References

  1. Brunt, A. A., Crabtree, K., Dallwitz, M. J., Gibbs, A. J., Watson, L. and Zucher, E. J. 1996. Plant viruses online: Descriptions and lists from the VIDE database Version: 20th August 1996. URL http://biology.anu.edu.au/Groups/MES/vide/.
  2. Clark, M. and Adams, A. 1977. Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. J. Gen. Virol. 34:475-483. https://doi.org/10.1099/0022-1317-34-3-475
  3. Choi, G. S., Kim, J. H., Kim, J. S. and Choi, J. K. 2004. Characterization of Cucumber mosaic virus Isolated from Water Chickweed (Stellaria aquatica). Plant Pathol. J. 20:131-134. https://doi.org/10.5423/PPJ.2004.20.2.131
  4. Choi, H. S., Ryu, J. K., Ahn, K. K., Cho, J. D. and Kim, J. S. 2001. Cucumber mosaic cucumovirus-CARNA5 causing bud necrosis on table tomato. Plant Pathol. J. 17:169-173.
  5. Choi, J. K., Kim, H. J., Hong, J. S., Kim, D. W. and Lee, S. Y. 1998. Identification and differentiation of cucumber mosaic virus isolates in Korea. Plant Pathol. J. 14:7-12.
  6. Choi, S. K., Choi, J. K., Park, W. M. and Ryu, K. H. 1999. RTPCR detection and identification of three species of cucumoviruses with a genus-specific single pair of primer. J. Virol. Methods 83:67-73. https://doi.org/10.1016/S0166-0934(99)00106-8
  7. Ding, S. W., Anderson, B. J., Haase, H. R. and Symons, R. H. 1994. New overlapping gene encoded by the cucumber mosaic virus genome. Virology 198:593-601. https://doi.org/10.1006/viro.1994.1071
  8. Ding, B., Li, Q., Nguyen, L., Palukaitis, P. and Lucas, W.J. 1995. Cucumber mosaic virus 3a protein potentiates cell-to-cell trafficking of CMV RNA in tobacco plants. Virology 207:345-53. https://doi.org/10.1006/viro.1995.1093
  9. Ding, S. W., Li, W. X. and Symons, R. H. 1995. A novel naturally occurring hybrid gene encoded by a plant RNA virus facilitates long distance virus movement. EMBO J. 14:5762-5772.
  10. Francki, R. I. 1985. Plant virus satellites. Annu. Rev. Microbiol. 39:151-174. https://doi.org/10.1146/annurev.mi.39.100185.001055
  11. Gal-On, A., Kaplan, I. B. and Palukaitis, P. 1996. Characterization of cucumber mosaic virus.II. Identification of movement protein sequences that influence its accumulation and systemic infection in tobacco. Virology 226:354-361. https://doi.org/10.1006/viro.1996.0663
  12. Gal-On, A., Kaplan, I. B., Roossinck, M. J. and Palukaitis, P. 1994. The kinetics of infection of zucchini squash by cucumber mosaic virus indicate a function for RNA 1 in virus movement. Virology 205:280-289. https://doi.org/10.1006/viro.1994.1644
  13. Hayes, R. J. and Buck, K. W. 1990. Complete replication of a eukaryotic virus RNA in vitro by a purified RNA-dependent RNA polymerase. Cell 63:263-368.
  14. Lee, H. G., Kim, S. R., Jeon, Y. W., Kwon, S. B., Ryu, K. H. and Choi, J. K. 2008. Identification and Characterization of three Isolates of Cucumber mosaic virus Isolated from Weed Hosts. Res. Plant Dis. 14:15-20. https://doi.org/10.5423/RPD.2008.14.1.015
  15. Logan, A. E., Zettler, F. W. and Christie, S. R. 1984. Susceptibility of Rudbeckia, Zinnia, Ageratum, and other Bedding plants to Bidens Mottle virus. Plant Dis. 68:260-262. https://doi.org/10.1094/PD-68-260
  16. Nitta, N., Takanami, Y., Kuwata, S. and Kubo, S. 1988. Comparative studies on the nucleotide sequence of cucumber mosaic virus RNA 3 between Y stain and Q strain. Ann. Phytopathol. Soc. Jpn. 54:516-522. https://doi.org/10.3186/jjphytopath.54.516
  17. Owen, J. and Palukaitis, P. 1988. Characterization of cucumber mosaic virus. I. Molecular heterogeneity mapping of RNA 3 in eight CMV strains. Virology 166:495-502. https://doi.org/10.1016/0042-6822(88)90520-X
  18. Owen, J., Shintaku, M., Aeschleman, P., Ben Tahar, S. and Palukaitis, P. 1990. Nucleotide sequence and evolutionary relationships of cucumber mosaic virus (CMV) strains: CMV RNA 3. J. Gen. Virol. 71:2243-2249.
  19. Palukaitis, P., Roossinck, M. J., Dietzgen, R. G. and Francki., R. I. 1992. Cucumber mosaic virus. Adv. Virus Res. 41:281-348.
  20. Roossinck, M. J. 2001. Cucumber mosaic virus, a model for RNA virus evolution. Mol. Plant Pathol. 2:59-63. https://doi.org/10.1046/j.1364-3703.2001.00058.x
  21. Roossinck, M. J. 2002. Evolutionary history of Cucumber mosaic virus deduced by phylogenetic analyses. J. Virol. 76:3382-3387. https://doi.org/10.1128/JVI.76.7.3382-3387.2002
  22. Roossinck, M. J., Bujarski, J. and Ding, S. W. 1999. Family Bromoviridae. 923-935.
  23. Shi, B. J., Miller, J., Symons, R. H. and Palukaitis, P. 2003. The 2b protein of cucumoviruses has a role in promoting the cell-tocell movement of pseudorecombinant viruses. Mol. Plant- Microbe Interact. 16:261-267. https://doi.org/10.1094/MPMI.2003.16.3.261
  24. Wang, Y., Tzfira, T., Gaba, V., Citovsky, V., P. Palukaitis and Gal- On. 2004. Functional analysis of the Cucumber mosaic virus 2b protein: pathogenicity and nuclear localization. J. Gen. Virol. 85:3135-3147. https://doi.org/10.1099/vir.0.80250-0

Cited by

  1. Identification, transmission and genomic characterization of a new member of the family Caulimoviridae causing a flower distortion disease of Rudbeckia hirta 2017, https://doi.org/10.1016/j.virusres.2017.05.012
  2. Characteristics of Cucumber mosaic virus isolated from Zea mays in Korea vol.27, pp.4, 2011, https://doi.org/10.5423/PPJ.2011.27.4.372
  3. First Report of Cucumber mosaic virus Isolated from Wild Vigna angularis var. nipponensis in Korea vol.30, pp.2, 2014, https://doi.org/10.5423/PPJ.NT.01.2013.0012