The Plant Pathology Journal

한국식물병리학회 (The Korean Society of Plant Pathology)
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Effect of Acibenzolar-S-methyl and Rahnella aquatilis (Ra39) on Chitinase and β-1, 3-glucanase Activities and Disease Resistance of Apple Plants

  • Abo-Elyousr, A.M. Kamal (Dept. of Plant Pathology Faculty of Agriculture, Assiut Univ.) ;
  • Sallam, M.A.A. (Dept. of Plant Pathology Faculty of Agriculture, Assiut Univ.) ;
  • Hassan, M.H.A. (Dept. of Plant Pathology Faculty of Agriculture, Assiut Univ.) ;
  • Zeller, W. (Institut fur biologischen Pflanzenschutz)
  • 투고 : 2009.10.11
  • 심사 : 2010.02.12
  • 발행 : 2010.03.01
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초록

The effect of Acibenzolar-S-methyl (ASM) and Rahnella aquatilis Ra39 against apple fire blight disease caused by Erwinia amylovora were tested as a possible alternative to streptomycin. In vitro studies, no inhibition effect against the pathogen was found when ASM was tested. Under greenhouse conditions, application of R. aquatilis Ra39 with the highly susceptible M26 rootstock resulted in a marked disease suppression. Application of ASM and strain Ra39 caused a high decrease of the disease, 82% and 58% respectively; this was correlated with a reduction of the growth of the pathogen within host plants up to 64% and 49.5% respectively. Further studies in the field under artificial infection condition during full bloom revealed that application of ASM and R. aquatilis Ra39 with Gala variety resulted in a control effect up to 21 and 29% respectively. In physiological studies, enhanced activities of PR-proteins (chitinase and $\beta$-1, 3-glucanase) were detected, which are well known as biochemical markers for systemic acquired resistance. Application of ASM to apple shoots caused the highest chitinase activity followed by strain Ra39. The enzyme activity was increased after 2, 4 and 6 days from application. In addition, ASM-treatment caused the higher $\beta$-1, 3-glucanase activity than strain Ra39. Maximum enzyme activity was recorded after 6 days from application and then decreased after 8 and 10 days from application.

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  2. Biological control of common blight of bean (Phaseolus vulgaris) causedbyXanthomonas axonopodispv.phaseoliby using the bacteriumRahnella aquatilis vol.44, pp.20, 2011, https://doi.org/10.1080/03235408.2010.544469
  3. Draft Genome Sequence of Rahnella aquatilis Strain HX2, a Plant Growth-Promoting Rhizobacterium Isolated from Vineyard Soil in Beijing, China vol.194, pp.23, 2012, https://doi.org/10.1128/JB.01769-12