Natural Product Sciences

  • 제16권3호
  • /
  • Pages.192-197
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  • 2010
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  • 1226-3907(pISSN)
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  • 2288-9027(eISSN)
한국생약학회 (The Korean Society of Pharmacognosy)
권호 표지

Anti-inflammatory and Anti-allergic Properties of Water Extract from the Seed of Phaseolus calcaratus Roxburgh

  • Fang, Minghao (Department of Orthodontics and Institute of Oral Biosciences, BK21 program and School of Dentistry, Chonbuk National University) ;
  • Cho, Hyoung-Kwon (Center for Health Care Technology development, HanPoong Pharmaceutical Co. Ltd.) ;
  • Ahn, Yun-Pyo (Department of Orthodontics and Institute of Oral Biosciences, BK21 program and School of Dentistry, Chonbuk National University) ;
  • Ro, Sang-Jeong (Department of Orthodontics and Institute of Oral Biosciences, BK21 program and School of Dentistry, Chonbuk National University) ;
  • Jeon, Young-Mi (Department of Orthodontics and Institute of Oral Biosciences, BK21 program and School of Dentistry, Chonbuk National University) ;
  • Lee, Jeong-Chae (Department of Orthodontics and Institute of Oral Biosciences, BK21 program and School of Dentistry, Chonbuk National University)
  • 투고 : 2010.07.02
  • 심사 : 2010.08.20
  • 발행 : 2010.09.30
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초록

The seeds of Raphanus sativus L. (RSL) and Phaseolus calcaratus Roxburgh (PHCR), the root of Scutellaria baicalensis (SB), and the flower of Lonicera japonica (LJ) have been traditionally used as herbal medicines for anti-inflammation. Unlike the SB and LJ, little information is available for the scientific bases that show the anti-inflammatory mechanisms of RSL and PHCR. In this study, we prepared boiled water extracts from the medicines and determined their potentials in inhibiting nitric oxide (NO) production, cyclooxygenase-2 (COX-2) expression, and tumor necrosis factor (TNF)-$\alpha$ and interleukin (IL)-6 secretion in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. The effects of the medicines on serum IgE levels in ovalbumin (OVA)-administrated mice were also studied. The medicines inhibited production of TNF-$\alpha$ and IL-6, and COX-2 expression in LPSstimulated macrophages. Especially, PHCR water extract showed more potent inhibition on TNF-$\alpha$ production than SB and LJ extracts, but RSL extract did not exert these effects. Similar to the cases of SB and LJ, PHCR extract prevented the phosphorylation of $I{\kappa}B{\alpha}$ and c-Jun, and the activation of NF-${\kappa}B$-DNA binding. Further, oral supplementation of PHCR extract attenuated significantly serum levels of total and OVA-specific IgE in OVAtreated animals. These results suggest a possibility that PHCR water extract can be used for the treatment of inflammatory and allergic diseases.

키워드

참고문헌

  1. Barillari, J., Iori, R., Papi, A., Orlandi, M., Bartolini, G., Gabbanini, S., Pedulli, G.F., and Valgimigli, L., Kaiware Daikon (Raphanus sativus L.) extract: a naturally multipotent chemopreventive agent. J. Agric. Food Chem. 56, 7823-7830 (2003).
  2. Gaestel, M., Mengel, A., Bothe, U., and Asadullah, K., Protein kinases as small molecule inhibitor targets in inflammation. Cur. Med. Chem. 14, 2214-2234 (2007). https://doi.org/10.2174/092986707781696636
  3. Kang, O.H., Choi, J.G., Lee, J.H., and Kwon, DY., Luteolin isolated from the flowers of Lonicera japonica suppresses inflammatory mediator release by blocking NF-kappaB and MAPKs activation pathways in HMC-1 cells. Molecules 15, 385-398 (2010). https://doi.org/10.3390/molecules15010385
  4. Kim, E.H., Shim, B., Kang, S., Jeong, G., Lee, J.S., Yu, Y.B., and Chun, M., Anti-inflammatory effects of Scutellaria baicalensis extract via suppression of immune modulators and MAP kinase signaling molecules. J. Ethnopharmacol. 126, 320-331 (2009). https://doi.org/10.1016/j.jep.2009.08.027
  5. Kwak, W.J., Han, C.K., Chang, H.W., Kim, H.P., Kang, S.S., and Son, K.H., Loniceroside C, an antiinflammatory saponin from Lonicera japonica. Chem. Pharm. Bull. (Tokyo) 51, 333-335 (2003). https://doi.org/10.1248/cpb.51.333
  6. Lin, C.C. and Shieh, D.E., The anti-inflammatory activity of Scutellaria rivularis extracts and its active components, baicalin, baicalein and wogonin. Am. J. Chin. Med. 24, 31-36 (1996). https://doi.org/10.1142/S0192415X96000050
  7. Li-Weber, M., New therapeutic aspects of flavones: The anticancer properties of Scutellaria and its main active constituents Wogonin, Baicalein and Baicalin. Cancer Treat. Rev. 35, 57-68 (2009). https://doi.org/10.1016/j.ctrv.2008.09.005
  8. Shih, H.C., Hsu, C.S., and Yang, L.L., In vitro study of the tocolytic effect of oroxylin A from Scutellaria baicalensis root. J. Biomed. Sci. 16, 27 (2009). https://doi.org/10.1186/1423-0127-16-27
  9. Tae, J., Han, S.W., Yoo, J.Y., Kim, J.A., Kang, O.H., Baek, O.S., Lim, J.P., Kim, D.K., Kim, Y.H., Bae, K.H., and Lee, Y.M., Antiinflammatory effect of Lonicera japonica in proteinase-activated receptor 2-mediated paw edema. Clin. Chim. Acta 330, 165-171 (2003). https://doi.org/10.1016/S0009-8981(03)00017-2
  10. Xagorari, A., Roussos, C., and Papapetropoulos, A., Inhibition of LPSstimulated pathways in macrophages by the flavonoid luteolin. Brit. J. Pharmacol. 136, 1058-1064 (2002). https://doi.org/10.1038/sj.bjp.0704803
  11. Xu, Y., Oliverson, B.G., and Simmons, D.L., Trifunctional inhibition of COX-2 by extracts of Lonicera japonica: direct inhibition, transcriptional and post-transcriptional down regulation. J. Ethnopharmacol. 111, 667-670 (2007). https://doi.org/10.1016/j.jep.2007.01.017
  12. Zhao, Q.W., Huang, X., Lou, Y.J., Weber, N., and Proksch, P., Effects of ethanol extracts from Adzuki bean (Phaseolus angularis Wight.) and Lima bean (Phaseolus lunatus L.) on estrogen and progesterone receptor phenotypes of MCF-7/BOS cells. Phytother. Res. 21, 648-652 (2007). https://doi.org/10.1002/ptr.2135