Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Enhancement of Anticarcinogenic Potentials of Submerged-Liquid Culture of Agaricus blazei Murill on Mouse Ascites Cancer by Rice Hull

왕겨에 의한 신령버섯균사체 액체배양액의 생쥐 항복수암성 증가

  • Kim, Young-S. (Division of Applied Life Science (BK21 programs)) ;
  • Jang, Wook-J. (Division of Applied Life Science (BK21 programs)) ;
  • Rakib, A. (Division of Applied Life Science (BK21 programs)) ;
  • Kwon, Jung-M. (Division of Applied Life Science (BK21 programs)) ;
  • Ahn, Chae-R. (Division of Applied Life Science (BK21 programs)) ;
  • Kim, So-Y. (Department of Food Science, International University of Korea) ;
  • Cho, Yong-U. (Department of Pharmaceutical Engineering, Jinju National University) ;
  • Ha, Young-K. (HK Biotech. Co., Ltd.) ;
  • Kim, Jeong-O. (HK Biotech. Co., Ltd.) ;
  • Ha, Yeong-L. (Division of Applied Life Science (BK21 programs))
  • 김영숙 (경상대학교 응용생명과학부(BK21)) ;
  • 장욱진 (경상대학교 응용생명과학부(BK21)) ;
  • 라키브 (경상대학교 응용생명과학부(BK21)) ;
  • 권정민 (경상대학교 응용생명과학부(BK21)) ;
  • 안채린 (경상대학교 응용생명과학부(BK21)) ;
  • 김소영 (한국국제대학교 식품과학과) ;
  • 조용운 (진주산업대학교 제약공학과) ;
  • 하영권 ((주) HK바이오텍) ;
  • 김정옥 ((주) HK바이오텍) ;
  • 하영래 (경상대학교 응용생명과학부(BK21))
  • Received : 2010.07.26
  • Accepted : 2010.08.27
  • Published : 2010.09.30
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Abstract

The effects of rice hull (RH) powder on the anticarcinogenic activity of submerged-liquid cultures of Agaricus blazei Murill (AB) were assessed for mouse ascites cancers induced by mouse Sarcoma S-180 (S-180) cancer cells. Optimal growth of AB mycelia in the basal liquid culture medium, containing soybean meal, was achieved by culturing at $25^{\circ}C$ for 5 days, when evaluated by $\beta$-glucan content, Brix, and mycelial weight, relative to other culture conditions. Hot-water extract (HWE) of the submergedliquid culture of AB mycelia grown at $25^{\circ}C$ for 5 days exhibited a stronger anticarcinogenic activity, relative to HWE from other culture conditions. No such effects were obtained from AB mycelial cultures by alternative temperature-controlling cultures. Both cytotoxicity for S-180 cells and anticarcinogenic potentials for mouse ascites cancer of the HWE from AB mycelia grown in the basal medium containing 1% RH powder for 5 days at $25^{\circ}C$ were significantly (p<0.05) enhanced, relative to HWE from the AB mycelia culture of the basal medium without RH powder. These results indicate that HWE of submerged-liquid culture of AB mycelia, incubated in media containing 1% RH powder at $25^{\circ}C$ for 5 days, enhanced anticarcinogenic activity against S-180 cell-induced mouse ascites cancer, and suggest that RH powder is an excellent ingredient for the improvement of the anticarcinogenic potentials of the submerged-liquid culture of mushroom mycelia.

왕겨가 Agaricus blazei Murill (AB: 신령버섯)균사체 액체배양 추출물의 항암성을 증가시키는지에 관한 연구를 수행하였다. AB균사체를 대두박을 기본으로 한 액체배지에 다양한 조건으로 배양하여, $\beta$-glucan 함량, Brix, 균사체를 측정하여 적정 생육조건을 선정하고, 이들의 열수추출물의 S-180 cell로 유도한 mouse 복수암에 대한 항암성을 조사하였다. AB균사체는 $25^{\circ}C$에서 5일간 배양하였을 때 최적 생육을 나타내었고, 이 배양물이 다른 조건에서 배양한 배양물보다 우수한 항암성을 나타내었다. AB균사체의 생육 및 항암성은 변온배양에 따른 효과는 없었다. 따라서 이 최적배양조건($25^{\circ}C$, 5일 배양)에서 AB균사체를 1% 왕겨분말이 함유된 액체배지에 배양하고, 이의 열수 추출물의 항암성을 검증하였다. 1% 왕겨가 함유된 액체배지에서 배양한 열수추출물의 항암성은 왕겨가 함유되지 않은 배지의 열수추출물보다 항암성이 유의성 있게 증가되었다(p<0.05). 왕겨의 첨가는 AB균사체의 생육을 오히려 촉진시켰다. 이 결과는 왕겨가 AB균사체 뿐 만 아니라 다른 버섯균사체 액체배양물의 항암성 증진을 위한 원료로 활용될 수 있을 것임을 의미한다.

Keywords

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