생명과학회지 (Journal of Life Science)

  • 제20권9호
  • /
  • Pages.1365-1372
  • /
  • 2010
  • /
  • 1225-9918(pISSN)
  • /
  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
권호 표지
DOI QR코드

DOI QR Code

건조 마 추출물의 항산화 및 인체 암세포 증식 억제 효과

Effects of Extracts from Dried Yam on Antioxidant and Growth of Human Cancer Cell Lines

  • 장주리 (한국해양대학교 해양환경생명과학부) ;
  • 황성연 (한국해양대학교 해양환경생명과학부) ;
  • 임선영 (한국해양대학교 해양환경생명과학부)
  • Jang, Joo-Ri (Division of Marine Environment & Bioscience, Korea Maritime University) ;
  • Hwang, Seong-Yeon (Division of Marine Environment & Bioscience, Korea Maritime University) ;
  • Lim, Sun-Young (Division of Marine Environment & Bioscience, Korea Maritime University)
  • 투고 : 2010.06.28
  • 심사 : 2010.08.27
  • 발행 : 2010.09.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

본 연구에서는 마를 건조 후 분말화하여 실험에 사용하였으며, 건조 마 분말에 대한 생리활성에 대한 연구로 건조된 마를 유기용매로 추출하여 마 추출물과 분획물들의 $H_2O_2$에 의해 유도된 산화적 스트레스 및 인체 암세포(HT1080 인체 섬유육종세포, HT-29 인체 결장암세포)에 대한 증식 억제 효과에 대해 검토하고자 하였다. 마는 항비만, 항변비, 항돌연변이, 혈당 및 혈중 콜레스테롤 감소 등의 활성이 있어 건강식품으로 알려져 있다. $H_2O_2$에 의해 유도된 산화적 스트레스 저해효과를 알아보기 위하여 DCFH-DA assay를 행하였다. 건조 마 A+M 추출물을 농도별로 인체 섬유육종세포(HT1080)에 처리하였을 때 농도 의존적으로 세포 내 활성산소종을 크게 억제시켰다. 각 분획물들 중 85% aq. MeOH 분획물은 다른 분획물과 비교하여 높은 지질 과산화물 생성 억제 효과를 보였다. 인체 암세포에 대한 증식 억제 실험에서 A+M 및 MeOH 추출물은 농도 의존적으로 인체 섬유육종 및 결장암세포의 증식을 억제하였다. 마 추출물로부터 얻어진 n-hexane, 85% aq. MeOH 및 n-butanol (n-BuOH) 분획물들은 첨가농도 0.5 mg/ml 이상 처리했을 때 이들 두 암세포의 증식을 유의적으로 억제하였다(p<0.05). 본 연구 결과로 부터 건조 마의 암세포 증식 억제 효과는 마의 A+M 추출물이 MeOH 추출물에 비해 높았고, 분획물들의 경우에는 water 분획물을 제외한 분획물들의 암세포 억제 효과가 높았다. 특히 85% aq. MeOH 분획물은 인체 암세포 증식 억제 효과뿐만 아니라 세포 내 활성산소종 감소시키는 효과가 높아 이들 분획물에 활성물질이 있을 것으로 추정된다.

We investigated the inhibitory effects of solvent extracts from dried yam on $H_2O_2$-induced oxidative stress and growth of cancer cell lines (HT1080 human fibrosarcoma and HT-29 human colon cancer cells). Yam (Dioscoreacea) has been recognized as a healthy food due to its various biological activities, such as anti-obesity, anti-constipation, anti-proliferation, and anti-mutagenic activities, as well as its ability to decrease blood glucose and cholesterol levels. In order to determine the protective effect on $H_2O_2$-induced oxidative stress, DCFH-DA (dichlorodihydrofluorescin diacetate) assay was conducted. Acetone with methylene chloride (A+M) extract of dried yam appeared to reduce the levels of intracellular reactive oxygen species (ROS) with dose responses. Among the fractions, 85% aq. methanol fraction showed the highest protective effect on production of lipid peroxides. Inhibitory effects of A+M and methanol (MeOH) extracts on the growth of HT1080 and HT-29 cancer cells increased in a dose dependent manner. The treatments of n-hexane, 85% aq. methanol and n-butanol fractions (${\geqq}0.5$ mg/ml concentrations) significantly inhibited the growth of both cancer cells (p<0.05). From these results, 85% aq. methanol fraction showed inhibitory effects on cellular oxidation and growth of human cancer cells, suggesting that this fraction may contain active compounds of dried yam.

키워드

참고문헌

  1. Ahn, J. H., K. H. Son, H. Y. Sohn, and S. T. Kwon. 2005. In vitro culture of adventitious roots from Dioscorea nipponica Makino for the production of steroidal saponins. Korean J. Plant Biotechnol. 32, 317-223.
  2. Ciacco, C. F. and B. L. D’appolonia. 1977. Characterization of starches from various tubers and their use in bread-baking. Cereal Chem. 54, 1096-1107.
  3. Denizot, F. and R. Lang. 1986. Rapid colorimetric assay for cell growth and survival. Modifications to the tetrazolium dye procedure giving improved sensitivity and reliablity. J. Immunol. Methods 89, 271-277. https://doi.org/10.1016/0022-1759(86)90368-6
  4. Ha, Y. D., S. P. Lee, and Y. G. Kwak. 1998. Removal of heavy metal and ACE inhibition of yam mucilage. J. Korean Soc. Food Sci. Nutr. 27, 751-756.
  5. Han, Y. N., S. H. Hahn, and I. R. Lee. 1990. Purification of mucilages from Dioscorea batatas and D. japonica and their content analysis. Korean J. Pharmacogn. 21, 274-283.
  6. Hironaka, K., K. Takada, and K. Ishilbashi. 1990. Chemical composition of mucilage of Chinese yam. Nippon Shokuhin Kogyo Gakkaishi 37, 48-51. https://doi.org/10.3136/nskkk1962.37.48
  7. Kang, T. H., S. Choi, T. Lee, M. Son, and S. Y. Kim. 2008, Characteristics of antidiabetic effect of Dioscorea rhizome (1) - Hypoglycemic effect. Korean J. Food Nutr. 21, 425-429.
  8. Kang, T. H., S. Choi, T. Lee, M. Son, J. Park, and S. Y. Kim. 2008. Characteristics of antidiabetic effect of Dioscorea rhizoma (2)-prevention of diabetic neuropathy by NGF induction. Korean J. Food Nutr. 21, 430-435.
  9. Kim, J. I., H. S. Jang, J. S. Kim, and H. Y. Sohn. 2009. Evaluation of antimicrobial, antithrombin and antioxidant activity of Dioscorea batatas Decne. Korean J. Microbiol. Biotechnol. 37, 133-139.
  10. Kim, K. K. 1999. Thermal characteristics of agriculture and fisheries by low temperature vacuum dryer. pp. 1-6, Proceedings of the KSME 1999 Spring Annual Meeting.
  11. Kim, K. K., B. Y. Sung, H. S. Jung, S. Y. Choi, and S. B. Moon. 2000. A study on the thermal characteristics of the large low temperature vacuum dryer for biological drying. J. Korean Soc. Marine Engineers 24, 427-434.
  12. Kim, M. W. 2001. Effects of $H_2O$-fraction of Dioscorea japonica Thunb and selenium on lipid peroxidation in streptozotocin- induced diabetic rats. Korean J. Soc. Food Cookery Sci. 17, 344-352.
  13. Kum, E. J., S. J. Park, B. H. Lee, J. S. Kim, K. H. Son, and H. Y. Sohn. 2006. Antifungal activity of phenanthrene derivatives from aerial bulbils of Dioscorea batatas Decne. J. Life Sci. 16, 647-652. https://doi.org/10.5352/JLS.2006.16.4.647
  14. Kwon, C. S., H. Y. Sohn, S. H. Kim, J. H. Kim, K. H. Son, J. S. Lee, J. K. Lim, and J. S. Kim. 2003. Anti-obesity effect of Dioscorea nipponica Makino with lipase-inhibitory activity in rodents. Biosci. Biotechnol. Biochem. 67, 1451-1456. https://doi.org/10.1271/bbb.67.1451
  15. Kwon, C. S., I. S. Son, H. Y. Shim, I. S. Kwun, and K. M. Chung. 1999. Effects of yam on lowering cholesterol level and its mechanism. Korean J. Food Nutr. 32, 637-643.
  16. Kwon, E. G., E. M. Choe, and S. J. Gu. 2001. Effects of mucilage from yam (Dioscorea batatas Decne) on blood glucose and lipid composition in alloxan-induced diabetic mice. Korean J. Food Sci. Technol. 33, 795-801.
  17. Kwon, J. H., G. D. Lee, S. J. Lee, S. K. Chung, and J. U. Choi. 1998. Changes in chemical components and physical properties with freeze dring and hot air-drying of Dioscorea batatas. J. Korean Soc. Food Sci. Nutr. 27, 908-913.
  18. LeBel, C. P., H. Ischiropoulos, and S. C. Bondy. 1992. Evaluation of the probe 2', 7'-Dichlorofluorescin as an indicator of reactive oxygen species formation and oxidative stress. Chem. Res. Toxicol. 5, 227-231. https://doi.org/10.1021/tx00026a012
  19. Lee, I. S., S. Y. Chung, C. S. Shim, and S. J. Koo. 1995. Inhibitory effect of yam (Dioscorea batatas Decne) extracts on the mutagenicity. Korean J. Soc. Food Sci. 11, 351-355.
  20. Lee, K. J., S. J. Hwang, J. H. Choi, and H. G. Jeong. 2008. Saponins derived from the roots of Platycodon grandiflorum inhibit HT-1080 cell invasion and MMPs activities: Regulation of NF-${\kappa}B$ activation via ROS signal pathway. Cancer Letters 268, 233-243. https://doi.org/10.1016/j.canlet.2008.03.058
  21. Ohtani, K. and K. Murakami. 1991. Structure of mannan fractionated from water soluble mucilage of nagaimo (Dioscorea batatas Decne). Agric Biol. Chem., 55, 2413-2414. https://doi.org/10.1271/bbb1961.55.2413
  22. Onayemi, O. 1986. Some chemical factors affecting the quality of processed yam. J. Food. Sci. 51, 161-164. https://doi.org/10.1111/j.1365-2621.1986.tb10860.x
  23. Ozo, O. N., J. C. caygill, and K. G. Coursey. 1984. Phenolics of five yam species. Pytochem. 23, 329-331. https://doi.org/10.1016/S0031-9422(00)80327-1
  24. Park, C. S., K. M. Yang, and M. L. Kim. 2006. Functional properties of medicinal plant extracts. Korean J. Food Cookery Sci. 23, 720-727.
  25. Pryored, W. A. 1984. Free radicals in biology. Academic Press. VI. 371.
  26. Ryu, H. Y., K. H. Bae, E. J. Kum, S. J. Park, B. H. Lee, and H. Y. Sohn. 2007. Evaluation for the antimicrobial, antioxidant and antithrombosis activity of natural spices for Fresh-cut yam. J. Life Sci. 17, 652-657. https://doi.org/10.5352/JLS.2007.17.5.652
  27. Shin, N. H. and S. J. Koo. 1998. Antimutagenic effect of dietary fiber from yam (Dioscorea batatas Decne) against 2-AF and MNNG. Korean J. Soc. Food Sci. 14, 333-338.
  28. Shin, S. R. 2004. Changes on the components of yam snack by processing methods. Korean J. Food Preserv. 11, 516-521.
  29. Tsuchiya, M., M. Suematsu, and H. Suzuki. 1994. In vivo visualization of oxygen radical-dependent photoemission. Methods Enzymol. 233, 128-140. https://doi.org/10.1016/S0076-6879(94)33015-8
  30. Tomoda, M., K. Ishikawa, and M. Yokoi. 1981. Plant mucilages. Isolation and characterization of a mucilage "Dioscorea-mucilage B" from the rhizophors of Dioscorea batatas. Chem. Pham. Bull. 29, 3256-3261. https://doi.org/10.1248/cpb.29.3256

피인용 문헌

  1. Larva of Lilioceris (Lilioceris) ruficollis (Coleoptera: Chrysomelidae) from Korea vol.51, pp.1, 2012, https://doi.org/10.5656/KSAE.2012.01.1.063
  2. Evaluation of Physicochemical Properties and Enhancement of Antioxidant Activities of Dioscorea batatas by Stepwise Steaming Process vol.25, pp.6, 2015, https://doi.org/10.17495/easdl.2015.12.25.6.1049
  3. Quality Characteristics of Pancake Premix with Dioscorea batatas Powder by Steaming Process vol.32, pp.5, 2016, https://doi.org/10.9724/kfcs.2016.32.5.593
  4. Effects of Turmeric (Curcuma longa L.) on Antioxidative Systems and Oxidative Damage in Rats Fed a High Fat and Cholesterol Diet vol.42, pp.4, 2013, https://doi.org/10.3746/jkfn.2013.42.4.570
  5. Effects of Water Extracts of Red Pepper Seeds Powder on Antioxidative Enzyme Activities and Oxidative Damage in Rats Fed High-Fat and High-Cholesterol Diets vol.44, pp.4, 2011, https://doi.org/10.4163/kjn.2011.44.4.284