Journal of the Korea Academia-Industrial cooperation Society (한국산학기술학회논문지)

The Korea Academia-Industrial cooperation Society (한국산학기술학회)
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Tyrosinase inhibitory activity and antimicrobial activity by mixtures of ultrasonicated chitosan and Maillard reaction products

초음파 처리 키토산과 메일라드 반응액 혼합물에 의한 tyrosinase 억제 활성 및 항균력 분석

  • Kim, Kyoung-Ja (Dept. of Biotechnology, College of Natural Science, Soonchunhyang University) ;
  • Yang, Yong-Joon (Dept. of Plant Science and Technology, Sangmyung University)
  • 김경자 (순천향대학교 자연과학대학 생명공학과) ;
  • 양용준 (상명대학교 식물산업공학과)
  • Received : 2010.05.03
  • Accepted : 2010.07.06
  • Published : 2010.07.31
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Abstract

The aim of this study was to investigate the tyrosinase inhibitory activity and antimicrobial activity by mixtures of ultrasonicated chitosan and Maillard reaction products. Analysis of tyrosinase was purified from potato and confirmed by active staining after SDS-PAGE. Maillard reaction products (MRPs) were formed from various sugars (glucose, fructose, galactose, xylose, arabinose or ribose) and cystein. MRPs inhibited the tyrosinase purified from potato. The highest tyrosinase inhibitory activity was shown by MRP from glucose and cystein. Ultrasonicated chitosan (over 1 hr) showed antimicrobial activity at the concentration of 1% against E. coli and S. aureus. For the development of antibrowning agent with antimicrobial activity, tyrosinase inhibitory and antimicrobial activity by the mixtures of ultrasonicated chitosan and MRP were tested. 1:1 mixture of ultrasonicated chitosan and MRP from glucose and cystein was the best antibrowning agent having antimicrobial activity.

본 연구의 목적은 초음파 처리 키토산과 메일라드 반응액 혼합물에 의한 tyrosinase 억제 활성과 항균력을 조사하고자 하였다. 감자로부터 tyrosinase를 분리하여 SDS-PAGE후 일반 단백질 염색과 tyrosinase 활성 염색을 행하여 tyrosinase를 확인하였다. 여러 가지 당 (glucose, fructose, galactose, xylose, arabinose or ribose)과 cystein과의 반응으로 생성된 메일라드 반응 산물의 tyrosinase 억제 활성을 비교한 결과, glucose과 cystein 반응으로 생성된 메일라드 반응 산물의 tyrosinase 억제 활성이 가장 높은 것으로 나타났다. 0.5% 아세트산에 녹인 1% 키토산을 시간별로 초음파 처리한 후 항균력을 조사한 결과 1 시간 이상 초음파 처리한 경우 E. coli 균주 와 S. aureus 균주에서 억제 활성을 보였다. 갈변 억제 활성과 항균력을 가진 혼합물 제조를 위하여 초음파 처리 키토산과 메일라드 반응액을 혼합비를 달리하여 조사한 결과 부피 비로 1 : 1 로 혼합한 경우가 tyrosinase 억제 활성과 항균력이 가장 효과가 높은 것으로 조사되었다.

Keywords

References

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