YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Proteome-based Identification of Proteins Regulated by Aldosterone in Rat Kidney Cortex

프로테오믹스 기술을 통한 쥐의 신장 피질에서 알도스테론(Aldosterone)에 의해 조절되는 단백질 동정

  • Song, Mi-Na (Department of Molecular Medicine, Department of Biochemistry, School of Medicine, Kyungpook National University) ;
  • Jeon, Hong-Bae (Department of Molecular Medicine, Department of Biochemistry, School of Medicine, Kyungpook National University) ;
  • Choi, Hyo-Jung (Department of Molecular Medicine, Department of Biochemistry, School of Medicine, Kyungpook National University) ;
  • Kwon, Tae-Hwan (Department of Molecular Medicine, Department of Biochemistry, School of Medicine, Kyungpook National University) ;
  • Baek, Moon-Chang (Department of Molecular Medicine, Department of Biochemistry, School of Medicine, Kyungpook National University)
  • 송미나 (경북대학교 의과대학 분자의학교실, 경북대학교 의과대학 생화학교실) ;
  • 전홍배 (경북대학교 의과대학 분자의학교실, 경북대학교 의과대학 생화학교실) ;
  • 최효정 (경북대학교 의과대학 분자의학교실, 경북대학교 의과대학 생화학교실) ;
  • 권태환 (경북대학교 의과대학 분자의학교실, 경북대학교 의과대학 생화학교실) ;
  • 백문창 (경북대학교 의과대학 분자의학교실, 경북대학교 의과대학 생화학교실)
  • Received : 2010.01.20
  • Accepted : 2010.04.30
  • Published : 2010.06.30
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Abstract

Aldosterone, mineralocorticoid hormone, has important functions related to the regulation of blood pressure and balance of fluids and electrolytes in the distal region of the nephron. By genomic and non-genomic action of aldosterone, the physiological kidney functions are modulated. However, many of them except several kind of sodium channel have not been identified and analyzed yet. In this study, proteomic technologies with two-dimensional gel electrophoresis (2-DE) gel using aldosterone rat model were applied to analyze and identify the aldosterone dependently expressed proteins in rat kidney cortex. As a result, the established aldosterone rat model exhibited the normal physiological responses to aldosterone and modulated proteins were identified, which included 15 increased and 3 decreased proteins on 2-DE analysis. Among them, 11 proteins were identified as changed proteins by LC-MS/MS analysis. These proteins identified as aldosterone induced proteins were involved in several cellular pathways such as cytoskeleton remodeling, energy metabolism, amino acid metabolism, and chaperone process. In conclusion, our data could provide the insights into the new mechanism underlying regulation of kidney functions by aldosterone.

Keywords

References

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