Vasodilation of Ethanol Extract of Cinnamomi Ramulus via Voltage Dependent $Ca^{2+}$ Channel Blockage

전압의존성 $Ca^{2+}$ 통로 억제를 통한 계지(桂枝) 에탄올 추출물의 혈관이완 효능

  • Kim, Jong-Bong (Department of Physiology, College of Oriental Medicine, Dongguk University) ;
  • Shin, Heung-Mook (Department of Physiology, College of Oriental Medicine, Dongguk University)
  • 김종봉 (동국대학교 한의과대학 생리학교실) ;
  • 신흥묵 (동국대학교 한의과대학 생리학교실)
  • Received : 2010.06.22
  • Accepted : 2010.07.16
  • Published : 2010.08.25

Abstract

Cinnamomi Ramulus is one of the medicinal plants that have been used to improve various diseases caused by insufficient blood circulation. This study was performed for the investigation of vasodilation efficacy ethanol extract of Cinnamomi Ramulus (CR). CR exhibited vascular relaxation against phenylephrine (PE, $10^{-6}M$)-, KCl- and NaF-induced contraction in rat thoracic aorta. In addition, its relaxation was endothelium-independent. Treatment of potassium channel blockers such as gilbenclamide (Gli, $10^{-5}M$), tetraethylammonium (TEA, 1 mM) and 4-aminopyridine (4-AP, 0.2 mM) did not effect on the relaxation of CR. The relaxant effects were also not inhibited by pre-treatment of rat aorta with L-NAME ($10^{-4}M$), methylene blue ($10^{-5}M$), indomethacin ($10^{-5}M$), and atropine ($10^{-6}M$). However, nifedipine ($10^{-5}M$), L-type $Ca^{2+}$ channel blocker, in part attenuated the relaxation of CR ($0.2\;mg/m{\ell}$), but SK&F96365 ($3{\times}10^{-5}M$), receptor activated $Ca^{2+}$ channel blocker and 2-APB ($10^{-4}M$), store operated $Ca^{2+}$ channel blocker did not affact dilation of CR. These findings suggest that the endothelium-independent relaxation effect of CR is partly related with inhibition of $Ca^{2+}$ influx via voltage dependent $Ca^{2+}$ channel.

Keywords

References

  1. Dart, A.M., Kingwell, B.A. Pulse pressure-a review of mechanism and clinical relevance. J Am Coll Cardiol. 37(4):975-984, 2001. https://doi.org/10.1016/S0735-1097(01)01108-1
  2. Peach, M.J., Loeb, A.L., Singer, J.A., Saye, J. Endothelium-derived vascular relaxing factor. Hypertension. 7(3):94-100, 1985.
  3. Carvagal, J.A., Germain, A.M., Huidobro-Toro, J.P., Weiner, C.P. Molecular mechanism of cGMP-mediated smooth muscle relaxation. J Cell Physiol. 184(3):409-420, 2000. https://doi.org/10.1002/1097-4652(200009)184:3<409::AID-JCP16>3.0.CO;2-K
  4. 辛民敎. 臨床本草學. 서울, 永林社, pp 310-312, 2002.
  5. 김호철. 한약약리학. 서울, 집문당, pp 66-67, 2001.
  6. 안규석, 정찬길, 문준전. 혈전증(血栓症)과 고점도혈증(高粘度血症)에 미치는 황기, 계지 및 홍화의 효능에 관한 실험적 연구. 대한동의병리학회지 4: 74-92, 1989.
  7. 박선동, 박원환, 김종구. 계지복령환(桂枝茯苓丸) 및 그 구성약물의 혈소판응집 억제에 관한 연구. 동국한의학연구소 논문집, 8(2):115-129, 2000.
  8. 강윤희, 최승훈, 안규석. 계지가 angiogenesisdm 억제기전에 미치는 영향. 동의병리학회지 13(2):41-53, 1999.
  9. 조려화, 이준경, 조국현, 강대길, 권태오, 권지웅, 김진숙, 손은진, 이호섭. 선학초 부탄올 추출물의 혈관 이완 효과의 기 전에 대한 연구. 생약학회지 37(2):67-73, 2006.
  10. 장기철, 이동웅. 백하수오 알칼로이드 성분의 혈관이완 효능. 생명과학회지 10(6):584-590, 2000.
  11. 유기량, 최호정, 김길훤, 신흥묵. 산사 부탄올 분획이 PGF2$\alpha$- 유도 혈관평활근수축의 억제에 미치는 신호전달 연구. 동의생리병리학회지 17(2):461-466, 2003.
  12. 김상대, 김길훤, 신흥묵. 川芎, 머위, 黃連추출물의 $Ca^{2+} $유입 억제를 통한 혈관 이완 효과. 동의생리병리학회지 20(6):1620 -1624, 2006.
  13. 채종구, 김길훤, 신흥묵. 내피세포 Nitric Oxide 유리를 통한 산사의 혈관 이완 작용. 동의생리병리학회지 17(1):146-150, 2003.
  14. Karaki, H., Ozaki, H., Hori, M., Mitsui-Saito M., Amano, K., Harada, K., Miyamoto, S., Nakazawa, H., Won, K.J., Sato, K. Calcium movements, distribution and functions in smooth muscle. Pharmacol. Rev. 49(2):157-230, 1997.
  15. Nevala, R., Paukku, K., Korpela, R., Vapaatalo, H. Calcium-sensitive potassium channel inhibitors antagonize genistein- and daidzein-induced arterial relaxation in vitro. Life Sci. 69(12):1407-1417, 2001. https://doi.org/10.1016/S0024-3205(01)01233-4
  16. Bolton, T.B. Mechanism of action of transmitters and other substances on smooth muscles. Physiological Reviews. 59: 606-718, 1979. https://doi.org/10.1152/physrev.1979.59.3.606
  17. Karaki, H., Weiss, G.B. Calcium channels in smooth muscle. Ggastroenterology. 87: 960-970, 1984.
  18. 이원로, 서정돈 외. 임상심장학. 서울, 고려의학, pp 69-97, 1998.
  19. Kwan, C.Y., Zhang, W.B., Kwan, T.K., Sakai, Y. In vitro relaxation of vascular smooth muscle by atropine: involvement of $K^+$ channels and endothelium. Naunyn-Schmiediegerg's Arch Parmacol. 368(1):1-9, 2003. https://doi.org/10.1007/s00210-003-0759-7