생명과학회지 (Journal of Life Science)

  • 제20권8호
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  • Pages.1249-1253
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  • 2010
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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갑상선세포에서 sericin에 의한 thyroglobulin의 분비증가

Sericin Enhances Secretion of Thyroglobulin in the Thyrocytes

  • 진초이 (충남대학교 의학전문대학원 해부학교실) ;
  • 송성희 (충남대학교 의학전문대학원 해부학교실) ;
  • 고영화 (충남대학교 의학전문대학원 해부학교실) ;
  • 권기상 (충남대학교 의학전문대학원 해부학교실) ;
  • 윤은영 (농촌진흥청 국립농업과학원) ;
  • 구태원 (농촌진흥청 국립농업과학원) ;
  • 여주홍 (농촌진흥청 국립농업과학원) ;
  • 김승환 (충남대학교병원 응급의학과) ;
  • 최종순 (한국기초과학연구원 생명과학연구부) ;
  • 유권 (한국생명공학연구원 장수과학연구센터) ;
  • 권오유 (충남대학교 의학전문대학원 해부학교실)
  • Jin, Cho-Yi (Department of Anatomy, School of Medicine, Chungnam National University) ;
  • Song, Seong-Hee (Department of Anatomy, School of Medicine, Chungnam National University) ;
  • Go, Young-Hwa (Department of Anatomy, School of Medicine, Chungnam National University) ;
  • Kwon, Ki-Sang (Department of Anatomy, School of Medicine, Chungnam National University) ;
  • Yun, Eun-Young (National Academy of Agricultural Science, R.D.A.) ;
  • Goo, Tae-Won (National Academy of Agricultural Science, R.D.A.) ;
  • Yeo, Joo-Hong (National Academy of Agricultural Science, R.D.A.) ;
  • Kim, Seung-Whan (Department of Emergency Medicine, Chungnam National University Hospital) ;
  • Choi, Jong-Soon (Division of Life Science, Korea Basic Science Institute) ;
  • Yu, Kweon (Korea Research Institute of Bioscience and Biotechnology) ;
  • Kwon, O-Yu (Department of Anatomy, School of Medicine, Chungnam National University)
  • 투고 : 2010.07.07
  • 심사 : 2010.07.15
  • 발행 : 2010.08.30
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초록

Sericin은 실크를 싸고 있는 고분자 수용성 당단백질로서 세포배양에 사용되며 세포분화를 촉진한다. 본 연구의 목적은 갑상선세포(FRTL-5)에서 thyroglobulin (Tg)의 분비에 sericin이 영향을 주는지를 알려고 한다. Sericin에 의해서 Tg의 분비가 촉진되었지만 Tg-mRNA의 발현은 촉진되지 않았다. 이런 상태에서 소포체 샤페론(Bip & calreticulin)과 소포체 막 단백질(IRE1, PERK & ATF6)의 발현이 증가한 것이 확인되었다. 한편 IRE1의 하부 신호전달자인 XBP1의 mRNA splicing 이 약하게 확인되었지만 PERK의 하부 신호전달자인 $eIF2{\alpha}$의 인산화는 일어나지 않았다. 그리고 sericin은 MTT assay 결과 cell viability을 촉진시키는 것도 확인되었다. 위의 결과는 sericin은 재조합 단백질생산에 유용하게 이용될 수 있는 새로운 생체물질로 증명되었다.

Sericin is a type of high molecular weight water-soluble glycoprotein surrounding fibroin (silk protein) that has been used as a cell culture supplement and accelerates cell proliferation in various serum-free media. The purpose of this study was to investigate the enhancing effect of thyroglobulin (Tg) secretion by sericin in thyrocytes, FRTL-5 cells. While Tg-mRNA expression was not enhanced, a secreted form of Tg was obviously increased by sericin. In this status, expression of both endoplasmic reticulum (ER) molecular chaperones (Bip & calreticulin) and ER membrane proteins (IRE1, PERK & ATF6) was enhanced. The proximal step of IRE1, XBP1 mRNA splicing was slightly detected however, the proximal step of PERK, phosphorylation of $eIF2{\alpha}$, was changeless. In addition, sericin enhanced cell viability by the MTT assay. The above results showing the ability of sericin to promote protein production demonstrated its potential usefulness as a new biomaterial.

키워드

참고문헌

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피인용 문헌

  1. Processing and characterization of silk sericin from Bombyx mori and its application in biomaterials and biomedicines vol.61, 2016, https://doi.org/10.1016/j.msec.2015.12.082
  2. The potential of silk sericin protein as a serum substitute or an additive in cell culture and cryopreservation vol.49, pp.6, 2017, https://doi.org/10.1007/s00726-017-2396-3