Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Analysis of Quantitative Trait Loci (QTLs) for Seed Size and Fatty Acid Composition Using Recombinant Inbred Lines in Soybean

콩 재조합자식계통을 이용한 콩 종자의 크기와 지방산 조성의 양적 형질 유전자좌 분석

  • Kim, Hyeun-Kyeung (Bioresources Development Institute, Pusan National University) ;
  • Kim, Yong-Chul (Department of Plant Bioscience, College of Natural Resource & Life Science, Pusan National University) ;
  • Kim, Sun-Tae (Department of Plant Bioscience, College of Natural Resource & Life Science, Pusan National University) ;
  • Son, Beung-Gu (Department of Horticulture Bioscience, College of Natural Resource & Life Science, Pusan National University) ;
  • Choi, Yong-Whan (Department of Horticulture Bioscience, College of Natural Resource & Life Science, Pusan National University) ;
  • Kang, Jum-Soon (Department of Horticulture Bioscience, College of Natural Resource & Life Science, Pusan National University) ;
  • Park, Young-Hoon (Department of Horticulture Bioscience, College of Natural Resource & Life Science, Pusan National University) ;
  • Cho, Young-Son (Department of Crop Science & Biotechnology, Jinju National University) ;
  • Choi, In-Soo (Department of Plant Bioscience, College of Natural Resource & Life Science, Pusan National University)
  • 김현경 (부산대학교 생명자원개발연구소) ;
  • 김용철 (부산대학교 생명자원과학대학 식물생명과학과) ;
  • 김선태 (부산대학교 생명자원과학대학 식물생명과학과) ;
  • 손병구 (부산대학교 생명자원과학대학 원예생명과학과) ;
  • 최영환 (부산대학교 생명자원과학대학 원예생명과학과) ;
  • 강점순 (부산대학교 생명자원과학대학 원예생명과학과) ;
  • 박영훈 (부산대학교 생명자원과학대학 원예생명과학과) ;
  • 조영손 (진주산업대학교 작물생명과학과) ;
  • 최인수 (부산대학교 생명자원과학대학 식물생명과학과)
  • Received : 2010.07.01
  • Accepted : 2010.08.26
  • Published : 2010.08.30
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Abstract

Soybean [Glycine max(L.) Merr.] is an important crop, accounting for 48% of the world market in oil crops. Improvements in economic traits, such as quality and oil constituents, arethe most important objectives in soybean breeding. The objective of this study was to identify quantitative trait loci (QTLs) that control seed size and fatty acid contents in soybean. 115 $F_{2:10}$ recombinant inbred lines (RIL) developed from a cross of 'Keunolkong' and 'Iksan10' were used. Narrow-sense heritability estimates based on a plot mean on 100 seed weight, saturated fatty acid (palmitic acid + stearic acid), and oleic, linoleic, and linolenic acid content were 0.72, 0.60, 0.83, 0.77 and 0.81, respectively. The 100 seeds weight was related to seven QTLs located on chromosomes 1, 3, 8, 9, 16 and 17. Two independent QTLs for saturated fatty acid content were identified on chromosomes 17 and 19. Five independent QTLs for oleic acid content wereidentified on chromosomes7, 11, 14, 16 and 19. Five QTLs for linoleic acid content were located on chromosomes 2, 11, 14, 16 and 19. Three QTLs for linolenic acid content were located on chromosomes 8, 10 and 19. Oleic, linoleic, and linolenic acid had one major common QTL on chromosome 19. Thus, linoleic and linolenic acid content were identified as common QTLs.

콩은 세계 유지작물시장에서 48%을 차지하는 중요한 작물이다. 콩 종실의 크기와 기름함량의 양적 및 질적 개선이 콩 육종에 있어서 가장 중요한 목적중의 하나이다. 이 연구의 목적은 종실의 크기와 지방산조성을 조절하는 양적 형질 유전자좌를 밝히는 것이다. 큰올콩과 익산10호의 교배로부터 F2:10 세대의 재조합자식계통 115계통을 이용하였다. 협의 유전력 검정에서는 백립중이 0.72, 포화지방산(팔미트산 + 스테아릭산)이 0.60, 올레익산이 0.83과 리놀레익산이 0.77 및 리놀렌산이 0.81을 나타내었다. 백립중과 연관된 양적형질유전자좌는 염색체 1번, 3번, 8번, 9번과 16번 및 17번에 7개로 나타났다. 포화지방산은 염색체 17번과 19번에 2개의 독립된 양적 형질 유전자좌가 연관되어 있었다. 올레익산 함량에 대해서는 다섯 개의 독립적인 양적 형질 유전자좌가 염색체 7번, 11번, 14번과 16번 및 19번에서 확인하였다. 리놀레익산 함량에 대한 5개의 양적 형질 유전자좌는 염색체 2번, 11번, 14번과 16번 및 19번에 있었다. 리놀렌산 함량은 3개의 양적형질유전자좌가 염색체 8번과 10번 및 19번에 관련되어 있었다. 그리고 올레익산과, 리놀레익산 및 리놀렌산에 공통적으로 확인되는 주요 양적 형질 유전자좌는 염색체 19번 이었다.

Keywords

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