Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Responses of Photosynthetic Efficiency and Ascorbate Peroxidase Induced by Salt Stress in Rice (Oryza sativa L.)

벼의 salt stress에 의해 유도된 산화 stress에 대한 ascorbate peroxidase 반응

  • Koo, Jeung-Suk (Department of Molecular Biology, Dong-eui University) ;
  • Im, Kyoung-Nam (Department of Molecular Biology, Dong-eui University) ;
  • Chun, Hyun-Sik (Department of Agronomy & Medicinal Plant Resources, Jinju National University) ;
  • Lee, Chin-Bum (Department of Molecular Biology, Dong-eui University)
  • 구정숙 (동의대학교 분자생물학과) ;
  • 임경남 (동의대학교 분자생물학과) ;
  • 전현식 (진주산업대학교 생명자원과학대학 농학한약자원학부) ;
  • 이진범 (동의대학교 분자생물학과)
  • Received : 2010.06.09
  • Accepted : 2010.07.03
  • Published : 2010.08.30
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Abstract

We investigated changes in photosynthesis and activity of ascorbate peroxidase (APX) that scavenges ROS as responses to oxidative stress induced by salinity in rice (Oryza sativa L.). Photosynthetic efficiency of rice leaves, monitored in terms of Fv/Fm, declined with the increase of salt concentration (100-300 mM NaCl). Salinity caused an increase of $H_2O_2$ in leaves of rice, with an increase of APX activity. Among total APX isoforms, an isoform of stromal-APX 1 in leaves of rice was completely inactivated by 300 mM NaCl, but was not affected by chilling or drought. The results suggest that salt stress acts in quite a different mechanism in relation to the activity of stromal-APX from that of other stresses such chilling and drought. We carried out RT-PCR for analysis of genes expression of APX isoforms as affected by salt stress. The expression of cytosolic APX/thylakoid-bound APX genes in leaves of rice exposed to salt stress was increased, while stromal APX gene expression rapidly declined.

Salt stress가 벼 잎 내 광합성 효율과 ascorbate peroxidase (APX) 활성에 미치는 영향을 조사하였다. 염 농도가 증가(NaCl, 100-300 mM) 할수록 Fv/Fm 값이 감소한 반면, $H_2O_2$ 양과 APX 활성은 증가하였다. APX isoforms 중 APX 1 (stromal)은 300 mM NaCl 처리 시 활성이 거의 나타나지 않는 반면, chilling이나 drought 처리 시에는 변화가 없었다. 또한 gel 상에서의 서로 다른 APX isoforms의 활성이 유전자 발현에서도 확인이 되는지를 알아보기 위해 RT-PCR을 수행하였다. 구획별 APX isoforms의 RT-PCR 수행 결과, cytosolic/thylakoid bound APX 발현양은 증가한 반면, stromal APX 발현은 매우 감소하였다. 이러한 실험 결과는 salt에 의해 구획별로 APX 활성이 영향을 받음을 나타낸다.

Keywords

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