Plant Biotechnology Reports

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Mitochondrial activity in illuminated leaves of chlorophyll-deficient mutant rice (OsCHLH) seedlings

  • Goh, Chang-Hyo (Environmental Biotechnology National Core Research Center, Gyeongsang National University) ;
  • Satoh, Kouji (Department of Molecular Genetics, National Institute of Agrobiological Sciences) ;
  • Kikuchi, Shoshi (Department of Molecular Genetics, National Institute of Agrobiological Sciences) ;
  • Kim, Seong-Cheol (National Institute of Subtropical Agriculture, Rural Development Administration) ;
  • Ko, Suk-Min (Subtropical Horticulture Research Institute, Jeju National University) ;
  • Kang, Hong-Gyu (Subtropical Horticulture Research Institute, Jeju National University) ;
  • Jeon, Jong-Seong (Plant Metabolism Research Center and Graduate School of Biotechnology, Kyung Hee University) ;
  • Kim, Cheol-Soo (Department of Plant Biotechnology and Agricultural Plant Stress Research Center, Chonnam National University) ;
  • Park, Youn-Il (Department of Biology, Chungnam National University)
  • Received : 2010.07.18
  • Accepted : 2010.07.29
  • Published : 2010.12.30
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Abstract

The rice CHLH gene encodes the $Mg^{2+}$-chelatase H subunit, which is involved in chlorophyll biosynthesis. Growth of the chlorophyll-deficient oschlh mutant is supported by mitochondrial activity. In this study, we investigated the activity of mitochondrial respiration in the illuminated leaves during oschlh seedling development. Growth of mutant plants was enhanced in the presence of 3% sucrose, which may be used by mitochondria to meet cellular energy requirements. ATP content in these mutants was, however, significantly lowered in light conditions. Low cytosolic levels of NADH in illuminated oschlh mutant leaves further indicated the inhibition of mitochondrial metabolism. This down-regulation was particularly evident for oxidative stressresponsive genes in the mutant under light conditions. Hydrogen peroxide levels were higher in oschlh mutant leaves than in wild-type leaves; this increase was largely caused by the impairment of the expression of the antioxidant genes, such as OsAPXl, OsRACl, and OsAOXc in knockout plants. Moreover, treatment of mesophyll protoplasts with ascorbic acid or catalase recovered ATP content in the mutants. Taken together, these results suggest that the light-mediated inhibition of mitochondrial activity leads to stunted growth of CHLH rice seedlings.

Keywords

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