Plant Biotechnology Reports

  • 제4권2호
  • /
  • Pages.173-178
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  • 2010
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  • 1863-5466(pISSN)
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  • 1863-5474(eISSN)
한국식물생명공학회 (The Korean Society of Plant Biotechnology)
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Shoot induction and regeneration using internodal transverse thin cell layer culture in Sesamum indicum L.

  • Chattopadhyaya, Banani (Department of Biotechnology and Adv. Lab. for Plant Genetic Engineering, Advanced Technology Development Center, Indian Institute of Technology) ;
  • Banerjee, Joydeep (Department of Biotechnology and Adv. Lab. for Plant Genetic Engineering, Advanced Technology Development Center, Indian Institute of Technology) ;
  • Basu, Asitava (Adv. Lab. for Plant Genetic Engineering, Advanced Technology Development Center, Indian Institute of Technology) ;
  • Sen, Soumitra K. (Adv. Lab. for Plant Genetic Engineering, Advanced Technology Development Center, Indian Institute of Technology) ;
  • Maiti, Mrinal K. (Department of Biotechnology and Adv. Lab. for Plant Genetic Engineering, Advanced Technology Development Center, Indian Institute of Technology)
  • 투고 : 2009.09.30
  • 심사 : 2010.02.05
  • 발행 : 2010.06.30
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초록

An efficient protocol for shoot regeneration was developed for sesame (Sesamum indicum L.) internodes using the transverse thin cell layer (tTCL) culture method. The frequency of shoot regeneration and the number of adventitious buds produced from regenerated shoots depend significantly on explant age, thickness of the tTCL sections, and the phytohormones supplemented to the culture medium. A combination of 6-benzyladenine (2.0 $mg\;l^{-1}$) and a-naphthaleneacetic acid (0.5 $mg\;l^{-1}$) was found to be the best phytohormone combination for shoot bud induction, with the maximum number of shoots obtained when the tTCL sections were 0.5-1.0 mm thick and derived from 4- to 6-week-old seedlings of sesame. Well-developed shoots were rooted on MS medium without phytohormones, and 80% of the regenerated plantlets were successfully established in soil.

키워드

참고문헌

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피인용 문헌

  1. Establishment of Sesame Callus Induction and Shoot Regeneration System : Establishment of Sesame Callus Induction and Shoot Regeneration System vol.47, pp.2, 2010, https://doi.org/10.3724/sp.j.1259.2012.00162
  2. An approach to standardize transient expression of GUS in sesame (Sesamum indicum L.) using the Sonication-Assisted Agrobacterium tumefaciens-mediated Transformation (SAAT) method vol.61, pp.1, 2013, https://doi.org/10.1080/07929978.2014.948275
  3. Plant Thin Cell Layers: A 40-Year Celebration vol.32, pp.4, 2010, https://doi.org/10.1007/s00344-013-9336-6
  4. A new high-frequency Agrobacterium-mediated transformation technique for Sesamum indicum L. using de-embryonated cotyledon as explant vol.251, pp.5, 2010, https://doi.org/10.1007/s00709-014-0625-0
  5. Genetic transformation of cultivated sesame (Sesamum indicum L. cv Rama) through particle bombardment using 5-day-old apical, meristematic tissues of germinating seedlings vol.123, pp.3, 2015, https://doi.org/10.1007/s11240-015-0848-6
  6. Development of reproducible regeneration and transformation system for Sesamum indicum vol.143, pp.2, 2010, https://doi.org/10.1007/s11240-020-01931-1