Plant Biotechnology Reports

The Korean Society of Plant Biotechnology (한국식물생명공학회)
권호 표지
DOI QR코드

DOI QR Code

Shoot multiplication kinetics and hyperhydric status of regenerated shoots of gladiolus in agar-solidified and matrix-supported liquid cultures

  • Gupta, S. Dutta (Department of Agricultural and Food Engineering, Indian Institute of Technology Kharagpur) ;
  • Prasad, V.S.S. (Department of Agricultural and Food Engineering, Indian Institute of Technology Kharagpur)
  • Received : 2009.04.27
  • Accepted : 2009.12.11
  • Published : 2010.03.30
Download PDF
⟨ Previous Next ⟩

Abstract

In vitro shoot regeneration of gladiolus in three different culture systems, viz., semi-solid agar (AS), membrane raft (MR), and duroplast foam liquid (DF) cultures was evaluated following the kinetics of shoot multiplication and hyperhydricity at optimized growth regulator combinations. Compared to the AS system, matrixsupported liquid cultures enhanced shoot multiplication. The peak of shoot multiplication rate was attained at 18 days of incubation in the MR and DF systems, whereas the maximum rate in the AS system was attained at 21 days. An early decline in acceleration trend was observed in liquid cultures than the AS culture. The hyperhydric status of the regenerated shoots in the different culture systems was assessed in terms of stomatal attributes and antioxidative status. Stomatal behavior appeared to be normal in the AS and MR systems. However, structural anomaly of stomata such as large, round shaped guard cells with damage in bordering regions of stomatal pores was pronounced in the DF system along with a relatively higher $K^+$ ion concentration than in the AS and MR systems. Antioxidative status of regenerated shoots was comparable in the AS and MR systems, while a higher incidence of oxidative damages of lipid membrane as evidenced from malondialdehyde and ascorbate content was observed in the DF system. Higher oxidative stress in the DF system was also apparent by elevated activities of superoxide dismutase, ascorbate peroxidase, and catalase. Among the three culture systems, liquid culture with MR resulted in maximum shoot multiplication with little or no symptoms of hyperhydricity. Shoots in the DF system were more prone to hyperhydricity than those in the AS and MR systems. The use of matrix support such as membrane raft as an interface between liquid medium and propagating tissue could be an effective means for rapid and efficient mass propagation with little or no symptoms of hyperhydricity.

Keywords

References

  1. Adelberg J (2006) Agitated thin-films of liquid media for efficient micropropagation. In: Dutta Gupta S, Ibaraki Y (eds) Plant tissue culture engineering. Springer, The Netherlands, pp 103-117
  2. Arora A, Sairam RK, Srivastava GC (2002) Oxidative stress and antioxidative system in plants. Curr Sci 82:1227-1238
  3. Bailly C, Benamar A, Corbineau F, Dome D (1996) Changes in malondialdehyde content and in superoxide dismutase, catalase and glutathione reductase activities in sunflower seed as related to deterioration during accelerated aging. Physiol Plant 97:104-110 https://doi.org/10.1111/j.1399-3054.1996.tb00485.x
  4. Beauchamp C, Fridovich I (1971) Superoxide dismutase: improved assays and an assay applicable to polyacrylamide gels. Anal Biochem 44:276-287 https://doi.org/10.1016/0003-2697(71)90370-8
  5. Beers RF, Sizer IW (1952) A spectrophotometric method for measuring breakdown of hydrogen peroxide by catalase. J Biol Chem 195:133-140
  6. Benson E (2000) Do free radicals have role in plant tissue culture recalcitrance? Special symposium: in vitro plant recalcitrance. In Vitro Cell Dev Biol-Plant 36:163-170
  7. Chakrabarty D, Park SY, Ali BM, Shin KS, Paek KY (2006) Hyperhydricity in apple: ultrastuctural and physiological aspects. Tree Physiol 26:377-388 https://doi.org/10.1093/treephys/26.3.377
  8. Chen SX, Schopfer P (1999) Hydroxyl-radical production in physiological reactions. A novel function of peroxidase. Eur J Biochem 260:726-735 https://doi.org/10.1046/j.1432-1327.1999.00199.x
  9. Chen J, Ziv M (2001) The effect of ancymidol on hyperhydricity, regeneration, starch and antioxidant enzymatic activities in liquid-cultured Narcissus. Plant Cell Rep 20:22-27 https://doi.org/10.1007/s002990000283
  10. Chen J, Ziv M (2004) Ancymidol-enhanced hyperhydric malformation in relation to gibberellin and oxidative stress in liquid cultured Narcissus leaves. In Vitro Cell Dev Biol-Plant 40:613-616 https://doi.org/10.1079/IVP2004579
  11. Debergh PC, Aitken-Christie J, Cohen D, Grout B, von Arnold S, Zimmerman R, Ziv M (1992) Reconsideration of the term 'vitrification' as used in micropropagation. Plant Cell Tissue Organ Cult 30:135-140 https://doi.org/10.1007/BF00034307
  12. Desamero NV, Adelberg JW, Hale SA, Young RE, Rhodes BB (1993) Nutrient utilization in liquid/membrane system for watermelon micropropagation. Plant Cell Tissue Organ Cult 33:265-271 https://doi.org/10.1007/BF02319011
  13. Dewir YH, Chakrabarty D, Ali MB, Hahna EJ, Paek KY (2006) Lipid peroxidation and antioxidant enzyme activities of Euphorbia millii hyperhydric shoots. Environ Exp Bot 58:93-99 https://doi.org/10.1016/j.envexpbot.2005.06.019
  14. Dutta Gupta S, Datta S (2004) Antioxidant enzyme activities during in vitro morphogenesis of gladiolus and the effect of application of antioxidants on plant regeneration. Biol Plant 47:179-183
  15. Fontes MA, Otoni WC, Carolino SMB, Brommonschenkel SH, Fontes EPB, Fari M, Louro RP (1999) Hyperhydricity in pepper plants regenerated in vitro: involvement of BiP (Binding Protein) and ultrastructural aspects. Plant Cell Rep 19:81-87 https://doi.org/10.1007/s002990050714
  16. Franck T, Kevers C, Penel C, Greppin H, Hausman JF, Gaspar T (1998) Reducing properties and markers of lipid peroxidation in normal and hyperhydrating shoots of Prunus avium L. J Plant Physiol 153:339-346 https://doi.org/10.1016/S0176-1617(98)80160-0
  17. Franck T, Kevers C, Gaspar T, Dommes J, Deby C, Greimers R, Serteyn D, Deby-Dupont G (2004) Hyperhydricity of Prunus avium shoots cultured on gelrite: a controlled stress response. Plant Physiol Biochem 42(6):519-527 https://doi.org/10.1016/j.plaphy.2004.05.003
  18. Gasper T, Penel C, Greppin H (1975) Peroxidase and isoperoxidases in relation to root and flower formation. Plant Biochem J 2:33-47
  19. Gressel J, Galun E (1994) Causes of photooxidative stress and amelioration of defence systems in plants. In: Foyer CH, Mullineaux PM (eds) CRC Press, Boca Raton, FL, pp 237-274
  20. Han BH, Paek KY, Choi JK (1992) Structural characteristics of vitrified and glaucous plantlets in Gypsophila paniculata L. in vitro. J Kor Soc Hortic Sci 33:177-189
  21. Hazarika BN (2006) Morpho-physiological disorders in in vitro culture of plants. Sci Hort 108:105-120 https://doi.org/10.1016/j.scienta.2006.01.038
  22. Heath RL, Packer L (1968) Photoperoxidation in isolated chloroplasts. I: Kinetics and stoichiometry of fatty acid peroxidation. Arch Biochem Biophys 125:189-198 https://doi.org/10.1016/0003-9861(68)90654-1
  23. Hodges DM, DeLong JM, Forney CF, Prange RK (1999) Improving the thiobarbituric acid-reactive-substances assay for estimating lipid peroxidation in plant tissues containing anthocyanin and other interfering compounds. Planta 207:604-611 https://doi.org/10.1007/s004250050524
  24. Kevers C, Franck T, Strasser RJ, Dimmes J, Gaspar T (2004) Hyperhydricity of micropropagated shoots: a typical stress induced state of physiological state. Plant Cell Tissue Organ Cult 77:181-191 https://doi.org/10.1023/B:TICU.0000016825.18930.e4
  25. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685 https://doi.org/10.1038/227680a0
  26. Law MY, Charles SA, Halliwell B (1983) Glutathione and ascorbic acid in spinach (Spinacia oleracea) chloroplasts. The effect of hydrogen peroxide and of paraquat. Biochem J 210:899-903 https://doi.org/10.1042/bj2100899
  27. Lin S, Zhang Z, Lin Y, Liu W, Guo H, Zhang W, Zhang C (2004) Comparative study on antioxidative system in normal and vitrified shoots of Populus suaveolens in tissue culture. For Stud China 6(3):1-8 https://doi.org/10.1007/s11632-004-0033-1
  28. Lowry DH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin-phenol reagent. J Biol Chem 193:265-295
  29. Mendes BMJ, Filippi SB, Demetrio CGB, Rodriguez APM (1999) A statistical approach to study the dynamics of micropropagation rates, using banana (Musa spp.) as an example. Plant Cell Rep 18:967-971 https://doi.org/10.1007/s002990050692
  30. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15:473-497 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  31. Nhut DT, Teixeira da Silva JA, Huyen PX, Paek KY (2004) The importance of explant source on regeneration and micropropagation of gladiolus by liquid shake culture. Sci Hort 102:407-414 https://doi.org/10.1016/j.scienta.2004.04.004
  32. Olmos E, Hellin E (1998) Ultrastructural differences in hyperhydric and normal leaves from regenerated carnation plants. Sci Hort 75:91-101 https://doi.org/10.1016/S0304-4238(98)00096-X
  33. Pandey S, Zhang W, Assmann SM (2007) Roles of ion channels and transporters in guard cell signal transduction. FEBS Lett 581:2325-2336 https://doi.org/10.1016/j.febslet.2007.04.008
  34. Papadakis AK, Roubelakis-Angelakis KA (2002) Oxidative stress could be responsible for the recalcitrance of plant protoplasts. Plant Physiol Biochem 40:549-559 https://doi.org/10.1016/S0981-9428(02)01423-7
  35. Picoli EAT, Otoni WC, Figueira ML, Carolino SMB, Almeida RS, Silva EAM, Carvalho CR, Fontes EPB (2001) Hyperhydricity in in vitro eggplant regenerated shoots: structural characteristics and involvement of BiP (Binding Protein). Plant Sci 160:857-868 https://doi.org/10.1016/S0168-9452(00)00463-5
  36. Piqueras A, Han BH, Van Huylenbroeck JM, Debergh PC (1998) Effect of different environmental conditions in vitro on sucrose metabolism and antioxidant enzymatic activities in cultured shoots of Nicotiana tabacum L. Plant Growth Reg 25:5-10 https://doi.org/10.1023/A:1005961632380
  37. Piqueras A, Cortina M, Serna MD, Casas JL (2002) Polyamines and hyperhydricity in micropropagated carnation shoots. Plant Sci 162:671-678 https://doi.org/10.1016/S0168-9452(02)00007-9
  38. Prasad VSS, Dutta Gupta S (2006) In vitro shoot regeneration of gladiolus in semi-solid agar versus liquid cultures with support systems. Plant Cell Tissue Organ Cult 87:263-271 https://doi.org/10.1007/s11240-006-9160-9
  39. Saher S, Piqueras A, Hellin E, Olmos E (2004) Hyperhydricity in micropropagated carnation shoots: the role of oxidative stress. Physiol Plant 120(1):152-161 https://doi.org/10.1111/j.0031-9317.2004.0219.x
  40. Saher S, Fernandez-Garcia N, Piqueras A, Hellin E, Olmos E (2005) Reducing properties, energy efficiency and carbohydrate metabolism in hyperhydric and normal carnation shoots cultured in vitro: a hypoxia stress? Plant Physiol Biochem 43(6):573-582 https://doi.org/10.1016/j.plaphy.2005.05.006
  41. Salisbury EJ (1927) On the causes and the ecological significance of stomatal frequency with special reference to teh woodland flora. Philos Trans R Soc Lond B 216:1-65
  42. Sampson J (1961) A method of replacing dry or moist surfaces for examination by light microscopes. Nature 191:932 https://doi.org/10.1038/191932a0
  43. Schroeder JI (2003) Knockout of the guard cell $K^{+}$ out channel and stomatal movements. Proc Natl Acad Sci USA 100:4976-4977 https://doi.org/10.1073/pnas.1031801100
  44. Steinitz BA, Cohen Z, Kochba GM (1991) Precocious gladiolus corm formation in liquid shake cultures. Plant Cell Tissue Organ Cult 26:63-70 https://doi.org/10.1007/BF00036107
  45. Ticha I, Radochova B, Kadlecck P (1999) Stomatal morphology during acclimatization of tobacco plantlets to ex vitro conditions. Biol Plant 42:469-474 https://doi.org/10.1023/A:1002450210939
  46. Werker E, Leshem B (1987) Structural changes during vitrification of carnation plantlets. Ann Bot 59:377-385 https://doi.org/10.1093/oxfordjournals.aob.a087327
  47. Ziv M (1989) Enhanced shoot and cormlet proliferation in liquid cultured Gladiolus buds by growth retardants. Plant Cell Tissue Organ Cult 17:101-110 https://doi.org/10.1007/BF00046855
  48. Ziv M (1991) Vitrification: morphological and physiological disorders of in vitro shoots. In: Debergh PC, Zimmerman RH (eds) Micropropagation. Kluwer, Dordrecht, pp 45-69
  49. Ziv M (2005) Simple bioreactors for mass propagation of plants. Plant Cell Tissue Organ Cult 8:277-285
  50. Ziv M, Ariel T (1994) Vitrification in relation to stomatal deformation and malfunction in carnation leaves in vitro. In: Lumsden PJ, Nicholas J, Davies WJ (eds) Physiology, growth and development of micropropagated plants. Kluwer, Dordrecht, pp 143-154
  51. Ziv M, Schwartz A, Fleminger D (1987) Malfunctioning stomata in vitreous leaves of carnation plants propagated in vitro: implication for hardening. Plant Sci 52:127-134 https://doi.org/10.1016/0168-9452(87)90114-2

Cited by

  1. Biochemical and morpho-anatomical analyses of strawberry vitroplants hyperhydric tissues affected by BA and gelling agents vol.60, pp.2, 2010, https://doi.org/10.1590/s0034-737x2013000200002
  2. Antioxidant enzyme activities, malondialdehyde, and total phenolic content of PEG-induced hyperhydric leaves in sugar beet tissue culture vol.49, pp.4, 2010, https://doi.org/10.1007/s11627-013-9511-2
  3. Machine vision based evaluation of impact of light emitting diodes (LEDs) on shoot regeneration and the effect of spectral quality on phenolic content and antioxidant capacity in Swertia chirata vol.174, pp.None, 2010, https://doi.org/10.1016/j.jphotobiol.2017.07.029