Plant Biotechnology Reports

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Rapid molecular authentication of three medicinal plant species, Cynanchum wilfordii, Cynanchum auriculatum, and Polygonum multiflorum (Fallopia multiflorum), by the development of RAPD-derived SCAR markers and multiplex-PCR

  • Moon, Byeong-Cheol (Center of Herbal Resources Research, Korea Institute of Oriental Medicine) ;
  • Choo, Byung-Kil (Department of Crop Agriculture and Life Science, Chonbuk National University) ;
  • Cheon, Myeong-Sook (Center of Herbal Resources Research, Korea Institute of Oriental Medicine) ;
  • Yoon, Tae-Sook (Center of Herbal Resources Research, Korea Institute of Oriental Medicine) ;
  • Ji, Yun-Ui (Center of Herbal Resources Research, Korea Institute of Oriental Medicine) ;
  • Kim, Bo-Bae (Center of Herbal Resources Research, Korea Institute of Oriental Medicine) ;
  • Lee, A-Young (Center of Herbal Resources Research, Korea Institute of Oriental Medicine) ;
  • Kim, Ho-Kyoung (Center of Herbal Resources Research, Korea Institute of Oriental Medicine)
  • Received : 2009.06.19
  • Accepted : 2009.08.29
  • Published : 2010.03.30
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Abstract

Definitive identification of original plant species is important for standardizing herbal medicine. The herbal medicines Cynanchi Wilfordii Radix (Baekshuoh in Korean and Beishuwu in Chinese) and Polygoni Multiflori Radix (Hashuoh in Korean and Heshuwu in Chinese) are often misidentified in the Korean herbal market due to morphological similarities and similar names. Therefore, we developed a reliable molecular marker for the identification of Cynanchi Wilfordii Radix and Polygoni Multiflori Radix. We used random amplified polymorphic DNA (RAPD) analysis of three plant species, Polygoni multiflorum, Cynanchum wilfordii, and Cynanchum auriculatum, to obtain several species-specific RAPD amplicons. From nucleotide sequences of these RAPD amplicons, we developed six sequence characterized amplification region (SCAR) markers for distinguishing Polygoni Multiflori Radix and Cynanchi Wilfordii Radix. Furthermore, we established SCAR markers for the simultaneous discrimination of the three species within a single reaction by using multiplex-PCR. These SCAR markers can be used for efficient and rapid authentication of these closely related species, and will be useful for preventing the distribution of adulterants.

Keywords

References

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