Effect of tissue culture medium waste after harvest of Korean wild ginseng on growth performance and diseases resistance in broiler chickens

산삼배양액 급여에 따른 육계의 생산성 및 질병 저항성 효과

  • Seol, Jae-Won (College of Veterinary Medicine, Chonbuk National University) ;
  • Park, Jae-Hong (Department of Animal Resources and Biotechnology, College of Agriculture & Life Science, Chonbuk National University) ;
  • Chae, Joon-Seok (College of Veterinary Medicine, Seoul National University) ;
  • Kang, Hyung-Sub (College of Veterinary Medicine, Chonbuk National University) ;
  • Ryu, Kyeong-Seon (Department of Animal Resources and Biotechnology, College of Agriculture & Life Science, Chonbuk National University) ;
  • Kang, Chun-Seong (E&T) ;
  • Park, Sang-Youel (College of Veterinary Medicine, Chonbuk National University)
  • Accepted : 2010.04.19
  • Published : 2010.06.30

Abstract

The large amount of tissue culture medium (TCM), which contains some of the active secretory components of Korean wild ginseng (KWG; Panax ginseng) such as saponins, is usually discarded after harvest of KWG. The present study was aimed to investigate the efficacy of oral administration of the TCM-KWG on growth performance and diseases resistance in broiler chickens. A day old broiler chickens randomized in 6 groups (n = 60/groups) were administered orally with 0, 2, 4, 8, 16 and 32 mL/L TCMKWG through drinking water for 5 weeks and examined the change of weight gain, feed intake and blood components. Also, five weeks old broiler chickens (n = 15/groups) were challenged orally with Salmonella (S.) gallinarum and investigated the mortality in broiler chickens. An average weight gain and feed intake significantly didn't change in TCM-KWG administration groups as compared to control group. The concentration of calcium (Ca), phosphate (Pi) and potassium (K) in serum were increase by TCM-KWG administration in broiler chickens. We also found that oral administration of TCM-KWG through drinking water significantly reduced the mortality in broiler chickens experimentally infected with virulent S. gallinarum. The results of this study indicated that TCM-KWG administration may elevate the resistance on disease and improved the skeleton formation and body homeostasis of chickens, and TCM-KWG can be used as a cost-effective and environmentally alternative additives to control of the disease and growth.

Keywords

References

  1. 김기석, 이회수, 모인필. 국내 닭에서의 가금티푸스 발생. 농업과학논문집 1995, 37, 544-549.
  2. 박경윤. 국내 가금류에서의 살모넬라감염증 발생상황과 살모넬라 분리주의 특성조사. 서울대학교 박사학위 논문. 서울대학교, 서울, 1999.
  3. 박노찬, 도재철, 조광현, 장성준, 권헌일, 박덕상. 닭티푸스의 발생상황과 Salmonella gallinarum의 항균제 감수성. 한국가축위생학회지 1995, 18, 113-123
  4. 박준철, 김영화, 정현정, 이성대, 장해동, 김인철, 이상진, 이재정, 이찬호, 이상석. 배양산삼 분말 및 그 발효산삼배양액 분말 첨가가 비육돈의 생산성 및 도체 특성에 미치는 영향. 한국동물자원과학회지 2006, 48, 819-826.
  5. 박한우, 김세창, 정노팔. 생쥐의 체액성 면역에 미치는 인삼사포닌 분획물들의 영향. 고려인삼학회지 1988, 12, 63-67.
  6. 장해동, 김해진, 민병준, 조진호, 진영걸, 유종상, 이재정, 한무호, 김인호. 발효산삼 배양액 부산물 급여가 비육돈의 생산성, 혈액성상, 육질특성 및 육내 ginsenoside 함량에 미치는 영향. 한국동물자원과학학회지 2007, 49, 329-340
  7. 진상근, 김일석, 김수정, 정기종, 이제룡. 산삼 배양액 급여 돈육의 지방산, 아미노산 조성 및 관능적 특성. 한국축산식품학회지 2006, 26, 349-355.
  8. Anadon A, Martinez-Larranaga MR. Residues of antimicrobial drugs and feed additives in animal products : regulatory aspects. Livest Prod Sci 1999, 59, 183-198. https://doi.org/10.1016/S0301-6226(99)00026-3
  9. Bae GS, Nam KP, Kim HS, Lee SG, Choi HS, Min WK, Joo W, Maeng WJ, Chang MB. Effects of the artificial culture medium of wild ginsengs on rumen fermentation characteristics in vitro. J Anim Sci & Technol 2003, 45, 987-996. https://doi.org/10.5187/JAST.2003.45.6.987
  10. Chen HL, Li DF, Chang BY, Gong LM, Dai JG, Yi GF. Effects of Chinese herbal polysaccharides on the immunity and growth performance of young broilers. Poult Sci 2003, 82, 364-370. https://doi.org/10.1093/ps/82.3.364
  11. Gao WY, Seon JH, Son SH, Malony M, Park KY. Production of pigment and alfa-tocopherol by cell cultures in safflower (Carthamus tinctorius L.). J Biotechnol 1999, 1, 69-77.
  12. Gillis CN. Panax ginseng pharmacology: a nitric oxide link? Biochem Pharmacol 1997, 54, 1-8. https://doi.org/10.1016/S0006-2952(97)00193-7
  13. Hernandez F, Madrid J, Garcia V, Orengo J, Megias MD. Influence of two plant extracts on broilers performance, digestibility, and digestive organ size. Poult Sci 2004, 83, 169-174. https://doi.org/10.1093/ps/83.2.169
  14. Mitsuhashi S, Harada K, Kameda M. On the drug-resistance of enteric bacteria. 6. Spontaneous and artificial elimination of transmissible drug-resistance factors. Jpn J Exp Med 1961, 31, 119-123.
  15. Oura H, Hiai S, Odaka Y, Yokozawa T. Studies on the biochemical action of ginseng saponin. I. Purification from ginseng extract of the active component stimulating serum protein biosynthesis. J Biochem 1975, 77, 1057-1065. https://doi.org/10.1093/oxfordjournals.jbchem.a130806
  16. Park YG, Son SH. Regeneration of plantlets from cell suspension culture derived callus of white poplar (Populus alba L.). Plant Cell Rep 1988, 7, 567-570. https://doi.org/10.1007/BF00272759
  17. Scaglione F, Ferrara F, Dugnani S, Falchi M, Santoro G, Fraschini F. Immunomodulatory effects of two extracts of Panax ginseng C.A. Meyer. Drugs Exp Clin Res 1990, 16, 537-542.
  18. Yun TK. Experimental and epidemiological evidence on non-organ specific cancer preventive effect of Korean ginseng and identification of active compounds. Mutat Res 2003, 523-524, 63-74. https://doi.org/10.1016/S0027-5107(02)00322-6