Biological characterization of Brucella spp. isolated from cattle in Gyeongbuk, Korea

국내 경북지역 소에서 분리된 브루셀라 분리주의 생물학적 특성

  • Kim, Jeong-Hwa (Northern Branch, Gyeongbuk Veterinary Service Laboratory) ;
  • Lim, Jeong Ju (College of Veterinary Medicine, Gyeongsang National University) ;
  • Kim, Dong Hyeok (College of Veterinary Medicine, Gyeongsang National University) ;
  • Lee, Jin Ju (College of Veterinary Medicine, Gyeongsang National University) ;
  • Kim, Dae Geun (College of Veterinary Medicine, Gyeongsang National University) ;
  • Jun, Moo-Hyung (College of Veterinary Medicine, Chungnam National University) ;
  • Kim, Sang Hun (College of Veterinary Medicine, Chungnam National University) ;
  • Chang, Hong Hee (Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Lee, Hu Jang (College of Veterinary Medicine, Gyeongsang National University) ;
  • Min, Won-Gi (College of Veterinary Medicine, Gyeongsang National University) ;
  • Kim, Suk (College of Veterinary Medicine, Gyeongsang National University)
  • 김정화 (경상북도 가축위생시험소 북부지소) ;
  • 임정주 (경상대학교 수의과대학) ;
  • 김동혁 (경상대학교 수의과대학) ;
  • 이진주 (경상대학교 수의과대학) ;
  • 김대근 (경상대학교 수의과대학) ;
  • 전무형 (충남대학교 수의과대학) ;
  • 김상훈 (충남대학교 수의과대학) ;
  • 장홍희 (경상대학교 농업생명과학연구원) ;
  • 이후장 (경상대학교 수의과대학) ;
  • 민원기 (경상대학교 수의과대학) ;
  • 김석 (경상대학교 수의과대학)
  • Accepted : 2010.02.25
  • Published : 2010.06.30

Abstract

Members of the genus Brucella are facultative intracellular bacteria and cause brucellosis, a chronic disease in humans and abortion in animals. In this study, we tested sera for brucellosis of 15 Hanwoo farms in the western part of Gyeong-buk province, resulting 5 farms were brucellosis positive in 2008. We collected blood from 277 heads in the brucellosis positive 5 farms, and performed serological diagnosis, brucella positive cattle which had shown higher than 200 antibody titer in tube agglutination test were slaughtered, supramammary lymph nodes were collected, and Brucella spp. wild type isolation and identification were performed. From these results, 15 of Brucella spp. wild type strains were isolated and all strains were identified as B. abortus biotype 1 by biological and molecular analysis. In the antimicrobial susceptibility test, all 15 strains had a similar susceptibility and resistance pattern. This study may be useful for bacteriological and epidemiological understanding of cattle brucellosis in Korea.

Keywords

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