Natural Product Sciences

The Korean Society of Pharmacognosy (한국생약학회)
권호 표지

Simultaneous Analysis of Bioactive Metabolites from Rehmannia glutinosa by HPLC-DAD-MS/MS

  • Won, Tae-Hyung (Natural Products Research Institute, College of Pharmacy, Seoul National University) ;
  • Ryu, Sung-Kwang (Natural Products Research Institute, College of Pharmacy, Seoul National University) ;
  • Kang, Sam-Sik (Natural Products Research Institute, College of Pharmacy, Seoul National University) ;
  • Shin, Jong-Heon (Natural Products Research Institute, College of Pharmacy, Seoul National University)
  • Received : 2010.06.06
  • Accepted : 2010.06.18
  • Published : 2010.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

A high-performance liquid chromatography (HPLC) with diode array detector (DAD) and electrospray ionization mass spectrometry (ESI-MS) was established for the simultaneous determination of five representative metabolites of the iridoid and phenolic classes from Rehmannia glutinosa. The optimal chromatographic conditions were obtained on an ODS column (5 mm, $4.6{\times}250\;mm$) with the column temperature at $25^{\circ}C$. The mobile phase was composed of water and acetonitrile using a gradient elution with the flow rate 0.3 mL/min. Detection wavelength was set at 205 nm. All calibration curves showed good linear regression ($r^2$ > 0.997) within test ranges. Limits of detection (LOD) and quantitation (LOQ) values were lower than 0.123 and $0.373\;{\mu}g/mL$, respectively. The developed method provided satisfactory precision and accuracy with overall intra-day and inter-day variations of 0.09 - 0.76% and 0.16 - 1.41%, respectively, and the overall recoveries of 99.03 - 102.67% for all of the compounds analyzed. In addition, effectiveness of diverse extraction methods was compared to each other for the development of standard analytic method. The verified method was successfully applied to the quantitative determination of five representative metabolites in twenty-one commercial Rehmannia glutinosa samples from different markets in Korea and China. The analytical results showed that the contents of the five analytes vary significantly with sources.

Keywords

References

  1. Chan K., Chinese medicinal materials and their interface with Western medical concepts. J. Ethnopharmacol. 96, 1-18 (2005). https://doi.org/10.1016/j.jep.2004.09.019
  2. Chao, J.C-J., Chiang, S.W., Wang, C.C., Tsai, Y.H., Wu, M.s., Hot waterextracted Lycium barbarum and Rehmannia glutinosa inhibit proliferation and induce apoptosis of hepatocellular carcinoma cells. World. J. Gastroentero. 12, 4478-4484 (2006). https://doi.org/10.3748/wjg.v12.i28.4478
  3. Chen, D.D., Dai, D.Z., Zhang, T., Rehmanniae suppresses the elevated activity of rat cerebral $ Ca^{2+}$,$ Mg^{2+]$-ATPase induced by isoprenaline. Pharmacol. Clin. Chin. Mater. Med. 12, 23-24 (1996).
  4. Chen, D.D., Dai, D.Z., Zhang, T., Rehmanniae regresses the cardiac hypertrophy and the elevated activity of rat cardium and cerebrum mitochondria $ Ca^{2+}$, $ Mg^{2+]$-ATPase induced by L-thyroxine. Pharmacol. Clin. Chin. Mater. Med. 13, 27-28 (1997).
  5. Chun, J.C., Kim, J.C., Hawng, I.T., Kim. S.E., Acteoside from Rehmannia glutinosa nullifies paraquat activity in Cucumis sativus. Pest. Biochem. Physiol. 72, 153-159 (2002). https://doi.org/10.1016/S0048-3575(02)00008-1
  6. Gao, X.D., Wu, W.T., Biological function of five Chinese traditional drugs on proliferation and IL-2 production of the mice lymphocytes. Chin. Pharm. J. 21, 43-45 (1990).
  7. Jiangsu Mew Medical college, Lexicon of Traditional Chinese Drugs. Shanghai People's press, Shanghai, pp.74-76 (1975).
  8. Kitagawa, I., Nishimura, T., Furubayashi, A., Yosioka, I., On the constituents of rhizoma of Rehmannia glutinosa Libosch. forma hueichingensis Hsiao. Yakugaku Zasshi 9, 593-596 (1971).
  9. Liang, A.H., Xue, B.Y., Wang, J.H., Hao, J.D., Yang, H., Yi, H., A study on hemostatic and immunologic actions of fresh- and dry Dihuang. Chin. J. Chin. Mater. Med. 24, 663-666 (1999).
  10. Morota, T., Sasaki, H., Nishimura, H., Sugama, K., Masao, C.C.Z, Mitsuhashi, H., Two iridoid glycosides from Rehmannia glutinosa. Phytochemistry 28, 2149-2153 (1989). https://doi.org/10.1016/S0031-9422(00)97934-2
  11. Oh, K.O., Kim, S.W., Kim, J.Y., Ko, S.Y., Kim, H.M., Baek, J.H., Ryoo, H.M., Kim, J.K., Effect of Rehmannia glutinosa Libosch. extracts on bone metabolism. Clin. Chim. Acta. 334, 185-195 (2003). https://doi.org/10.1016/S0009-8981(03)00238-9
  12. Normile, M., The new face of traditional Chinese medicine. Science 299, 188-190 (2003). https://doi.org/10.1126/science.299.5604.188
  13. Oshio, H., Inouye, H., Iridoid glycosides of Rehmannia glutinosa. Phytochemistry 21, 133-138 (1982). https://doi.org/10.1016/0031-9422(82)80029-0
  14. Oshio, H., Naruse, Y., Inouye, H., Quantitative analysis of iridoid glycosides of Rehmannia glutinosa. Phytochemistry 21, 133-138 (1981).
  15. Qian, Z.-M., Li, H.-J., Li, P., Ren, M.-T., Tang, D., Simultaneous qualitation and quantification of thirteen bioactive compounds in Flos Lonicerae by high-performance liquid chromatography with diode array detector and mass spectrometry. Chem. Pharm. Bull. 55, 1073- 1076 (2007). https://doi.org/10.1248/cpb.55.1073
  16. Shoyama, Y., Matsumoto, M., Nishioka, I., Four caffeoyl glycosides form callus tissue of Rehmannia glutinosa. Phytochemistry 25, 1633-1636 (1986). https://doi.org/10.1016/S0031-9422(00)81224-8
  17. Tomoda, M., Kato, S., Onuma, M., Water-soluble constituents of Rehmanniae radix. On the consistents of roots of Rehmannia glutinosa var.purpurea. Chem. Pharm. Bull. 19, 2411-2416 (1971). https://doi.org/10.1248/cpb.19.2411
  18. Toshiko, H., Katsuya, K., Shuhichi, T., Utako, A., Constituents of leaves and root of kaikei jio. Shoyakugaku Zashi 36, 1-5 (1982).
  19. Wu, S.J., Xu, S.M., Li, Y.C., Study of chemical constituents from Rehmannia glutinosa f. hueichingensis. Chin. Trad. Herb Drugs. 15, 6-8 (1984).
  20. Xue, T.H. and Roy, R., Studying traditional Chinese medicine. Science 300, 740-741 (2003). https://doi.org/10.1126/science.300.5620.740
  21. Zhang, G.J., Ji, J.Y., Liu, Q., Zhang, L.H., Gong, G.Y., Jin, Z.X., Ren, S.z., Collected Edition of Identification and Assessment of Common TCD. Heilongjiang Science and Technique Press, Harbin, China, pp.301-302 (1993).
  22. Zhang, R.X., Li, M.X., Jia, Z.P., Rehmannia glutinosa: Review of botany, chemistry and pharmacology. J. Ethnopharmacol. 117, 199-214 (2008). https://doi.org/10.1016/j.jep.2008.02.018