Natural Product Sciences

  • 제16권2호
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  • Pages.88-92
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  • 2010
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  • 1226-3907(pISSN)
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  • 2288-9027(eISSN)
한국생약학회 (The Korean Society of Pharmacognosy)
권호 표지

Eudesmols Induce Apoptosis through Release of Cytochrome c in HL-60 Cells

  • Hoang, Duc Manh (Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Trung, Trinh Nam (College of Pharmacy, Chungnam National University) ;
  • He, Long (Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Ha, Do Thi (College of Pharmacy, Chungnam National University) ;
  • Lee, Myoung-Sook (Research Institute of Obesity Science, Sungshin Women's University) ;
  • Kim, Bo-Yeon (Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Luong, Hoang Van (Vietnam Military Medical University) ;
  • Ahn, Jong-Seog (Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Bae, Ki-Hwan (College of Pharmacy, Chungnam National University)
  • 투고 : 2010.04.15
  • 심사 : 2010.05.15
  • 발행 : 2010.06.30
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초록

We verified that the apoptosis activities were examined by DNA fragmentation, flow cytometric analysis with annexin V staining, activation of caspase-3, and cytochrome c release. In the result, $\alpha$- and $\beta$-eudesmol induced DNA fragmentation in HL-60 cells at a concentration of $80\;{\mu}M$, respectively. Additionally, pro-apoptotic cells sorted by flow cytometry analysis were detected in HL-60 cells to 31.77 and 29.67% with $\acute{a}$- and $\beta$-eudesmol of $80\;{\mu}M$. Thus, both $\alpha$- and $\beta$-eudesmol exerted caspase-3 activation and cytochrome c release at $80\;{\mu}M$ in HL-60 cells. These results are firstly reported that the sesquiterpenes, $\alpha$- and $\beta$-eudesmol are apoptosis inducers through mitochondria-dependent caspase cascade in HL-60 cells.

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참고문헌

  1. Al-Dabbas, M.M., Suganuma, T., Kitahara, K., Hou, D.X., and Fujii, M., Cytotoxic, antioxidant and antibacterial activities of Varthemia iphionoides Boiss. extracts. J Ethnopharmacol. 108, 287-293 (2006). https://doi.org/10.1016/j.jep.2006.05.006
  2. Bae, E.A., Han, M.J., Kim, N.J., and Kim, D.H., Anti-Helicobacter pylori activity of herbal medicines. Biol. Pharm. Bull. 21, 990-992 (1998). https://doi.org/10.1248/bpb.21.990
  3. Denyer, C.V., Jackson, P., Loakers, D.M., Ellis, M.R., and Young, D. A.B., Isolation of antirhinoviral sesquiterpenes from ginger (Zingiber officinale). J. Nat. Prod. 57, 658-662 (1994). https://doi.org/10.1021/np50107a017
  4. El-Gammal, M.H. and Wolff, P., A further contribution to the sesquiterpenoid constituents of Cymbopogon proximus. Planta Med., 53, 293-294 (1987). https://doi.org/10.1055/s-2006-962712
  5. Hamasaki, Y., Kobayashi, I., Zaitu, M., Tsuji, K., Kita, M., Hayasaki, R., Muro, E., Yamamoto S., Matsuo, M., Ichimaru, T., and Miyazaki, S., Magnolol inhibits leukotriene synthesis in rat basophilic leukemia- 2H3 cells. Planta Med., 65, 222-226 (1999). https://doi.org/10.1055/s-1999-13984
  6. Ikeda, K. and Nagase, H., Magnolol has the ability to induce apoptosis in tumor cells. Biol. Pharm. Bull., 25, 1546-1549 (2002). https://doi.org/10.1248/bpb.25.1546
  7. Kimura, M., Kimura, I., Kondoh, T., and Tsuneki, H., Noncontractile acetylcholine receptor-operated $ Ca^{++} $mobilization: suppression of activation by open channel blockers and acceleration of desensitization by closed channel blockers in mouse diaphragm muscle. J. Pharmacol. Exp. Ther., 256, 18-23 (1991).
  8. Raharivelomanana, P., Bianchini, J.P., Ramanoelina, A.R.P., Rasoarahona, J.R.E., Faure, R., and Cambon, A., Eudesmane sesquiterpenes from Laggera alata. Phytochemistry. 47, 1085-1088 (1998). https://doi.org/10.1016/S0031-9422(98)80077-0
  9. Sun, Z., Chen, B., Zhang, S., and Hu C., Four new eudesmanes from Caragana intermedia and their biological activities. J Nat Prod. 67, 1975-1979 (2004). https://doi.org/10.1021/np049866z
  10. Teng, C.M., Yu, S.M., Chen, C.C., Huang, Y.L., and Huang ,T.F., EDRFrelease and $ Ca^{+(+)}$-channel blockade by magnolol, an antiplatelet agent isolated from Chinese herb Magnolia officinalis, in rat thoracic aorta. Life Sci., 47, 1153-1161 (1990). https://doi.org/10.1016/0024-3205(90)90176-R
  11. Vera, N., Misico, R., Sierra, M.G., Asakawa, Y., and Bardon, A., Eudesmanes from Pluchea sagittalis. Their antifeedant activity on Spodoptera frugiperda. Phytochemistry. 69, 1689-94 (2008). https://doi.org/10.1016/j.phytochem.2008.02.020