Eudesmols Induce Apoptosis through Release of Cytochrome c in HL-60 Cells

  • Hoang, Duc Manh (Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Trung, Trinh Nam (College of Pharmacy, Chungnam National University) ;
  • He, Long (Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Ha, Do Thi (College of Pharmacy, Chungnam National University) ;
  • Lee, Myoung-Sook (Research Institute of Obesity Science, Sungshin Women's University) ;
  • Kim, Bo-Yeon (Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Luong, Hoang Van (Vietnam Military Medical University) ;
  • Ahn, Jong-Seog (Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Bae, Ki-Hwan (College of Pharmacy, Chungnam National University)
  • Received : 2010.04.15
  • Accepted : 2010.05.15
  • Published : 2010.06.30

Abstract

We verified that the apoptosis activities were examined by DNA fragmentation, flow cytometric analysis with annexin V staining, activation of caspase-3, and cytochrome c release. In the result, $\alpha$- and $\beta$-eudesmol induced DNA fragmentation in HL-60 cells at a concentration of $80\;{\mu}M$, respectively. Additionally, pro-apoptotic cells sorted by flow cytometry analysis were detected in HL-60 cells to 31.77 and 29.67% with $\acute{a}$- and $\beta$-eudesmol of $80\;{\mu}M$. Thus, both $\alpha$- and $\beta$-eudesmol exerted caspase-3 activation and cytochrome c release at $80\;{\mu}M$ in HL-60 cells. These results are firstly reported that the sesquiterpenes, $\alpha$- and $\beta$-eudesmol are apoptosis inducers through mitochondria-dependent caspase cascade in HL-60 cells.

Keywords

References

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