감초, 겨자 및 키토산을 첨가한 저염 된장의 발효 특성

Fermentation Properties of Low-Salted Doenjang Supplemented with Licorice, Mustard, and Chitosan

  • 투고 : 2009.12.29
  • 심사 : 2010.02.15
  • 발행 : 2010.06.30

초록

감초, 겨자, 키토산을 첨가하고 전통된장의 염도를 30% 낮춘 된장을 제조하여 된장의 발효특성을 조사하였다. 염도를 12.7%에서 10.2, 8.9%로 낮춘 된장에 감초, 겨자, 키토산 등 첨가물의 첨가는 된장의 내부의 균수, 색도, 산도에 거의 영향을 미치지 않았다. 저염된장은 된장표면에 white pellicle-forming strain이 발생하였으나 첨가물을 함유하고 있는 된장에서는 발생되지 않았다. 발효 20일째의 첨가물을 첨가한 염도 8.9% 된장의 아미노태 질소함량은 332 mg%로서 발효 40일의 염도 12.7%의 된장과 유사하였다. 관능평가에서는 첨가물을 첨가한 저염된장에서 구수한 맛 등이 높게 평가되어 종합적 기호도가 가장 높게 나타났다. 이러한 결과로부터 감초, 겨자, 키토산의 첨가는 된장의 발효시간을 단축시키고 기호성을 향상시킬 수 있으며 된장의 염도를 8.9%까지 낮출 수 있을 것으로 판단된다.

After supplemention with the licorice (Glycyrrhiza glabra), mustard (Brassica juncea), and chitosan as food additives to low-salted doenjang containing 30% lower salt than control doenjang (12.7% salt), fermentation properties of doenjang were investigated for 40 days. Adding the licorice, mustard, and chitosan to low-salted doenjangs containing 10.2% and 8.9% of salt did not affect the acidity, viable cell count, or color of doenjang. A white pellicle-forming strain was detected at the surface of low-salted doenjangs (10.2% and 8.9% salt) but not the control doenjang and low-salted doenjangs added with mixed additives (licorice, mustard, and chitosan). The amino nitrogen content of 8.9% salted doenjang added with mixed additives at 20 days was 332 mg% and this value was similar to that of 12.7% salted doenjang at 40 days. In sensory evaluation, the 8.9% salted doenjang added the additives had the highest score in overall palatability. These results indicate that salt contents of doenjang could be lowered to 8.9% by adding licorice, mustard, and chitosan, resulting in improved palatability, shortened fermentation period, and inhibited abnormal fermentation.

키워드

참고문헌

  1. Dahl LK. Possible role of salt intake in the development of essential hypertension. Int. J. Epidemiol. 34: 967-972 (2005) https://doi.org/10.1093/ije/dyh317
  2. Law M. Salt, blood pressure, and cardiovascular diseases. J. Cardiovasc. Risk 7: 5-8 (2000)
  3. Tsugane S. Salt, salted food intake, and risk of gastric cancer: Epidemiologic evidence. Cancer Sci. 96: 1-6 (2005) https://doi.org/10.1111/j.1349-7006.2005.00006.x
  4. Sellmeyer DE, Schloetter M, Sebastian A. Potassium citrate prevents increased urine calcium excretion and bone resorption induced by a high sodium chloride diet. J. Clin. Endoc. Metab. 87: 2008-2012 (2002) https://doi.org/10.1210/jc.87.5.2008
  5. Ministry of Health and welfare. The 3rd National Health and Nutrition Examination (KNHANES III). Ministry of Health and welfare, Seoul, Korea. p. 366 (2006)
  6. Nam HW, Lee GY. A study on the sodium and potassium intake and their metabolism of the pregnant women in the Korea. Korean J. Nutr. 18: 194-200 (1985)
  7. Yamamoto Y, Higashi K, Yoshii H. Inhivitory activity of ethanol on food spoilage bacteria. Nippon Shokuhin Kogyo Gakk. 31: 531-535 (1984) https://doi.org/10.3136/nskkk1962.31.8_531
  8. Lee KS, Kim DH, Moon CO. Effect of ethanol and lactic acid on the preparation of low salted kochujang. Theses Collection of Wonkwang Univ., Iksan, Korea 20: 143-164 (1986)
  9. Kim DH, Lee JS. Effect of condiments on the physicochemical characteristics of traditional kochujang during fermentation. Korean J. Food Sci. Technol. 33: 353-360 (2001)
  10. Yun YS, Kim KS, Lee YN. Antibacterial and antifungal effect of chitosan. J. Chitin Chitosan 4: 8-14 (1999)
  11. Shin DH, Ahn EY, Kim YS, Oh JA. Fermentation characteristics of kochujang containing horseradish or mustard. Korean J. Food Sci. Technol. 32: 1350-1357 (2000)
  12. Kim DH, Kwon YM. Effect of storage conditions on the microbiological and physicochemical characteristics of traditional kochujang. Korean J. Food Sci. Technol. 33: 589-595 (2001)
  13. Ahn EU, Shin DH, Baek NI, Oh JA. Isolation and identification of antimicrobial active substance from Glycyrrhiza uralensis FISCH. Korean J. Food Sci. Technol. 30: 680-687 (1998)
  14. Kim SJ, Shin JY, Park YM, Chung KM, Lee JH, Kweon DH. Investigation of antimicrobial activity and stability of ethanol extracts of licorice root (Glycyrrhiza glabra). Korean J. Food Sci. Technol. 38: 241-248 (2006)
  15. Tokuoka K, Mori R, Isshiki K. Inhibitory effects of volatile mustard extract on the growth of yeasts. Nippon Shokuhin Kogyo Gakk. 39: 68-73 (1992) https://doi.org/10.3136/nskkk1962.39.68
  16. Shofran BG, Purrington ST, Bredt F, Fleming HP. Antimicrobial properties of sinigrin and its hydrolysis products. J. Food Sci. 63: 621-624 (1998)
  17. Lim SI, Kwak EJ, Choi C. Isolation of the white pellicle-forming Strain from gochujang, growth inhibition, and its effect on quality of gochujang. Food Sci. Biotechnol. 13: 75-79 (2004)
  18. Shahiya F, Arachchi JKV, You JJ. Food applications of chitin and chitosans. Trends Food Sci. Technol. 10: 37-51 (1999) https://doi.org/10.1016/S0924-2244(99)00017-5
  19. Chun KH, Kim BY, Hahm YT. Extension of tofu shelf-life with water soluble degraded chitosan as a coagulant. J. Korean Soc. Food Sci. Nutr. 28: 161-166 (1999)
  20. Choi IW, Jung CH, Park YK. Anticariogenic activities of various plant extracts. Korean J. Food Sci. Technol. 35: 1221-1225 (2003)
  21. Lim SI, Lee BY. Effect of licorice (Glycyrrhiza glabra) ethanol extract on growth of Bacillus cereus at meju producing stage. Korean Soc. Appl. Bio. Chem. 53: 184-191 (2010) https://doi.org/10.3839/jksabc.2010.030
  22. Park JS, Lee MR, Kim JS, Lee TS. Compositions of nitrogen compound and amino acid in soybean paste (doenjang) prepared with different micobial sources. Korean J. Food Sci. Technol. 26: 609-615 (1994)
  23. Park WP, Kim ND, Lee SC, Kim SY, Cho SH. Effect of powder and concentrates of Prnus mume on the quality of doenjang during fermentation. Korean J. Food Preserv. 13: 574-580 (2006)
  24. Kim JS, Choi SH, Lee SD, Lee GH, Oh MJ. Quality changes of sterilized soybean paste during its storage. J. Korean Soc. Food Sci. Nutr. 28: 1069-1075 (1999)
  25. Mok CK, Song KT, Lee JY, Park YS, Lim SB. Changes in microorganism and enzyme activity of low salt soybean paste (doenjang) during fermentation. Food Eng. Prog. 9: 112-117 (2005)
  26. Kwak EJ, Lim SI. Effect of food additives on inhibiting the browning of model solution for doenjang. J. Korean Soc. Food Sci. Nutr. 36: 589-594 (2007) https://doi.org/10.3746/jkfn.2007.36.5.589
  27. Kim ND. Study on the browning and it's inhibition in soybean paste (doenjang). PhD thesis, Kon-Kuk Univ., Seoul, Korea (1996)
  28. Rhee CH, Kim WC, Rhee IK, Park HD. Effects of inoculation of Bacillus subtilis cells on the fermentation of Korean traditional soy paste (doenjang). Korean J. Food Preserv. 15: 598-605 (2008)