동의생리병리학회지 (Journal of Physiology & Pathology in Korean Medicine)

  • 제24권2호
  • /
  • Pages.220-227
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  • 2010
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  • 1738-7698(pISSN)
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  • 2288-2529(eISSN)
대한동의생리학회·한의병리학회 ( The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine)
권호 표지

갈근(葛根) 추출물이 cisplatin에 의해 유도된 rat mesangial cell의 apoptosis에 미치는 영향

Effects of Puerariae Radix extract on Cisplatin-Induced Apoptosis of Rat Mesangial Cells

  • 홍재의 (원광대학교 한의과대학 폐계내과학교실) ;
  • 신조영 (원광대학교 한의과대학 폐계내과학교실) ;
  • 주성민 (원광대학교 한의과대학 병리학교실) ;
  • 전병훈 (원광대학교 한의과대학 병리학교실) ;
  • 이시형 (원광대학교 한의과대학 폐계내과학교실)
  • Hong, Jae-Eui (Department of Internal Medicine, Wonkwang University) ;
  • Shin, Jo-Young (Department of Internal Medicine, Wonkwang University) ;
  • Ju, Sung-Min (Department of Pathology, College of Oriental Medicine, Wonkwang University) ;
  • Jeon, Byung-Hun (Department of Pathology, College of Oriental Medicine, Wonkwang University) ;
  • Lee, Si-Hyeong (Department of Internal Medicine, Wonkwang University)
  • 투고 : 2010.03.16
  • 심사 : 2010.04.13
  • 발행 : 2010.04.25
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초록

Puerariae radix (PR) is a popular natural herb and a traditional food in Asia, which has antithrombotic and anti-allergic properties and stimulates estrogenic activity. One of the major side effects of cisplatin is nephrotoxicity, leading to acute renal failure. Recent study has suggested a role of ROS and p53 in renal cell injury by cisplatin. We studied that protective effects of PR on cisplatin-induced apoptosis in rat mesangial cell. Rat mesangial cell was preincubated with PR (50, 100, 150 and 200 ${\mu}g/m{\ell}$) for 12 hr and then treated with 30 ${\mu}M$ cisplatin for 24 hr. Protective effect of PR on cisplatin-induced apoptosis in ECV304 cells was determined using MTT assay, FDA-PI staining, flow cytometric analysis, caspase-3 activity assay, ROS assay and western blot. Our results showed that PR inhibited in cisplatin-induced apoptosis and ROS production in ECV304 cells. Moreover, PR reduced ERK, p38 and JNK activation that increased in cisplatin-treated rat mesangial cell. Furthermore, activation of p53 by cisplatin in rat mesangial cell was inhibited by PR treatment. These results suggest that protective effect of PR on cisplatin-induced apoptosis in rat mesangial cell may be associated with reduction of ERK, p38, JNK, p53 activation.

키워드

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