Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Effects of Puerariae Radix extract on Cisplatin-Induced Apoptosis of Rat Mesangial Cells

갈근(葛根) 추출물이 cisplatin에 의해 유도된 rat mesangial cell의 apoptosis에 미치는 영향

  • Hong, Jae-Eui (Department of Internal Medicine, Wonkwang University) ;
  • Shin, Jo-Young (Department of Internal Medicine, Wonkwang University) ;
  • Ju, Sung-Min (Department of Pathology, College of Oriental Medicine, Wonkwang University) ;
  • Jeon, Byung-Hun (Department of Pathology, College of Oriental Medicine, Wonkwang University) ;
  • Lee, Si-Hyeong (Department of Internal Medicine, Wonkwang University)
  • 홍재의 (원광대학교 한의과대학 폐계내과학교실) ;
  • 신조영 (원광대학교 한의과대학 폐계내과학교실) ;
  • 주성민 (원광대학교 한의과대학 병리학교실) ;
  • 전병훈 (원광대학교 한의과대학 병리학교실) ;
  • 이시형 (원광대학교 한의과대학 폐계내과학교실)
  • Received : 2010.03.16
  • Accepted : 2010.04.13
  • Published : 2010.04.25
Download PDF
⟨ Previous Next ⟩

Abstract

Puerariae radix (PR) is a popular natural herb and a traditional food in Asia, which has antithrombotic and anti-allergic properties and stimulates estrogenic activity. One of the major side effects of cisplatin is nephrotoxicity, leading to acute renal failure. Recent study has suggested a role of ROS and p53 in renal cell injury by cisplatin. We studied that protective effects of PR on cisplatin-induced apoptosis in rat mesangial cell. Rat mesangial cell was preincubated with PR (50, 100, 150 and 200 ${\mu}g/m{\ell}$) for 12 hr and then treated with 30 ${\mu}M$ cisplatin for 24 hr. Protective effect of PR on cisplatin-induced apoptosis in ECV304 cells was determined using MTT assay, FDA-PI staining, flow cytometric analysis, caspase-3 activity assay, ROS assay and western blot. Our results showed that PR inhibited in cisplatin-induced apoptosis and ROS production in ECV304 cells. Moreover, PR reduced ERK, p38 and JNK activation that increased in cisplatin-treated rat mesangial cell. Furthermore, activation of p53 by cisplatin in rat mesangial cell was inhibited by PR treatment. These results suggest that protective effect of PR on cisplatin-induced apoptosis in rat mesangial cell may be associated with reduction of ERK, p38, JNK, p53 activation.

Keywords

References

  1. Timmer-Bosscha, H., Mulder, N.H., de Vries, E.G. Modulation of cis-diamminedichloroplatinum(II) resistance: a review. Br J Cancer. 66: 227-238, 1992. https://doi.org/10.1038/bjc.1992.249
  2. Goldstein, R.S., Mayor, G.H. Minireview. The nephrotoxicity of cisplatin. Life Sci. 32: 685-690, 1983. https://doi.org/10.1016/0024-3205(83)90299-0
  3. Lieberthal, W., Triaca, V., Levine, J. Mechanisms of death induced by cisplatin in proximal tubular epithelial cells: apoptosis vs. necrosis. Am. J. Physiol. 270: 700-708. 1996.
  4. Cummings, B.S., Schnellmann, R.G. Cisplatin-induced renal cell apoptosis: caspase 3-dependent and -independent pathways. J. Pharmacol. Exp. Ther. 302: 8-17, 2002. https://doi.org/10.1124/jpet.302.1.8
  5. Jiang, M., Yi, X., Hsu, S., Wang, C.Y., Dong, Z. Role of p53 in cisplatin-induced tubular cell apoptosis: dependence on p53 transcriptional activity. Am. J. Physiol. Renal. Physiol. 287: 1140-1147, 2004. https://doi.org/10.1152/ajprenal.00262.2004
  6. Baliga, R., Ueda, N., Walker, P.D., Shah, S. Oxidant mechanisms in toxic acute renal failure. Drug Metab. Rev. 31: 971-997, 1999. https://doi.org/10.1081/DMR-100101947
  7. Nath, K.A., Norby, S.M. Reactive oxygen species and acute renal failure. Am. J. Med. 109: 665-678, 2000. https://doi.org/10.1016/S0002-9343(00)00612-4
  8. Martindale, J.L., Holbrook, N.J. Cellular response to oxidative stress: signaling for suicide and survival. J. Cell Physiol. 192: 1-15, 2000.
  9. Taguchi. T., Nazneen, A., Abid, M.R., Razzaque, M.S. Cisplatin-associated nephrotoxicity and pathological events. Contrib. Nephrol. 148: 107-121. 2005.
  10. Baek, S.M., Kwon, C.H., Kim, J.H., Woo, J.S., Jung, J.S., Kim, Y.K. Differential roles of hydrogen peroxide and hydroxyl radical in cisplatin-induced cell death in renal proximal tubular epithelial cells. J. Lab. Clin. Med. 142: 178-186, 2003. https://doi.org/10.1016/S0022-2143(03)00111-2
  11. Matsushima, H., Yonemura, K., Ohishi, K., Hishida, A. The role of oxygen free radicals in cisplatin-induced acute renal failure in rats. J. Lab. Clin. Med. 131: 518-526, 1998. https://doi.org/10.1016/S0022-2143(98)90060-9
  12. Sueishi, K., Mishima, K., Makino, K., Itoh, Y., Tsuruya, K., Hirakata, H., et al. Protection by a radical scavenger edaravone against cisplatin-induced nephrotoxicity in rats. Eur. J. Pharmacol. 451: 203-208, 2002. https://doi.org/10.1016/S0014-2999(02)02251-3
  13. Tsuruya, K., Tokumoto, M., Ninomiya, T., Hirakawa, M., Masutani, K., Taniguchi, M., et al. Antioxidant ameliorates cisplatin-induced renal tubular cell death through inhibition of death receptor-mediated pathways. Am. J. Physiol. Renal. Physiol. 285: 208-218, 2003. https://doi.org/10.1152/ajprenal.00311.2002
  14. 김창민, 신민교, 안덕균, 이경순. 중약대사전. 서울, 정담, pp 33-40, 1998.
  15. 조수열, 장주연, 김명주. 갈화와 갈근 열수추출물이 에탄올투여 흰쥐의 혈청성분에 미치는 영향. 한국식품영양과학회지 30: 92-96, 2001.
  16. 부일권, 김연섭. 갈근이 뇌허혈 손상 흰쥐의 해마신경세포 손상에 미치는 영향. 대한본초학회지 19: 77-82. 2004.
  17. Yu, H.H., Seo, S.J., Moon, H.D., Park, R.K., So, H.S., Jeon, B.H., Jung, S.Y., You, Y.O. Protective effect of pueraria radix extract on the cisplatin-induced cytotoxicity of HEI-OC1 cells via scavenging of free radicals. Kor. J. Orien. Physiol. Pathol. 21: 462-467, 2007.
  18. 신민교. 임상본초학. 서울, 도서출판 영림사, pp 341-343, 2002.
  19. 강소신의학원편. 중약대사전. 서울, 도서출판 정담, pp 45-54, 1998.
  20. 徐樹楠, 牛兵占[共]編. 神農本草經. 石家庄, 河北科學技术出版社, p 66, 1996.
  21. 중화본초편찬위. 중화본초. 상해, 상해과학기술출판사, pp 610-619, 2002.
  22. 허 준. 동의보감. 경남, 동의보감출판사, p 195, 467, 977, 1137, 2167, 2005.
  23. Oh, M.J., Lee, K.S., Son, H.Y., Kim, S.Y. Antioxidative components of Pueraria root. Korean J. Food Sci. Technol. 22: 793-798, 1995.
  24. Lee, J.S., Lee, K.H., Jeong, J.H. Effects of extracts of Puerariae Radix on lipid metabolism in rats fed high fat diet. J. Korean Soc. Food Sci. Nutr. 28: 218-224, 1999.
  25. Eastman, A. The formation, isolation and characterization of DNA adducts produced by anticancer platinum complexes. Pharmacol. Ther. 34: 155-166, 1987. https://doi.org/10.1016/0163-7258(87)90009-X
  26. Siddik, Z.H. Cisplatin: mode of cytotoxic action and molecular basis of resistance. Oncogene. 22: 7265-7279, 2003. https://doi.org/10.1038/sj.onc.1206933
  27. Evan, G., Littlewood, T.A. Matter of life and cell death. Science, 281: 1317-1322, 1998. https://doi.org/10.1126/science.281.5381.1317
  28. Thornberry, N.A., Lazebnik, Y. Caspases: enemies within. Science, 281: 1312-1316, 1998. https://doi.org/10.1126/science.281.5381.1312
  29. Piao, W., Yoo, J., Lee, D.K., Hwang, H.J., Kim, J.H. Induction of G(2)/M phase arrest and apoptosis by a new synthetic anti-cancer agent, DW2282, in promyelocytic leukemia (HL-60) cells. Biochem. Pharmacol., 62: 1439-1447, 2001. https://doi.org/10.1016/S0006-2952(01)00796-1
  30. Cohen, J.J. Apoptosis. Immunol. 14: 126-130, 1993.
  31. Cross, T.G., Scheel-Toellner, D., Henriquez, N.V., Deacon, E., Salmon, M., Lord, J.M. Serine/threonine protein kinases and apoptosis. Exp. Cell Res. 256: 34-41, 2000. https://doi.org/10.1006/excr.2000.4836
  32. Pearson, G., Robinson, F., Beers Gibson, T., Xu, B.E., Karandikar, M., Berman, K., Cobb, M.H. Mitogen-activated protein (MAP) kinase pathways: regulation and physiological functions. Endocr. Rev. 22: 153-183, 2001. https://doi.org/10.1210/er.22.2.153
  33. Cobb, M.H. MAP kinase pathways. Prog. Biophys. Mol. Biol. 71: 479-500, 1999. https://doi.org/10.1016/S0079-6107(98)00056-X
  34. Xia, Z., Dickens, M., Raingeaud, J., Davis, R.J., Greenberg, M.E. Opposing effects of ERK and JNK-p38 MAP kinases on apoptosis. Science. 270: 1326-1331, 1995. https://doi.org/10.1126/science.270.5240.1326
  35. Davis, R.J. Signal transduction by the JNK group of MAP kinases. Cell. 103: 239-252, 2000. https://doi.org/10.1016/S0092-8674(00)00116-1
  36. Fan, M., Chambers, T.C. Role of mitogen-activated protein kinases in the response of tumor cells to chemotherapy. Drug Resist. Updat. 4: 253-267, 2001. https://doi.org/10.1054/drup.2001.0214
  37. Sanchez-Perez, I., Murguia, J.R., Perona, R. Cisplatin induces a persistent activation of JNK that is related to cell death. Oncogene 16: 533-540, 1998. https://doi.org/10.1038/sj.onc.1201578
  38. Zanke, B.W., Boudreau, K., Rubie, E., Winnett, E., Tibbles, L.A., Zon, L., Kyriakis, J., Liu, F.F., Woodgett, J.R. The stress-activated protein kinase pathway mediates cell death following injury induced by cis-platinum, UV irradiation or heat. Curr. Biol. 6: 606-613, 1996. https://doi.org/10.1016/S0960-9822(02)00547-X
  39. Sanchez-Perez, I., Perona, R. Lack of c-Jun activity increases survival to cisplatin. FEBS Lett. 453: 151-158, 1999. https://doi.org/10.1016/S0014-5793(99)00690-0
  40. Mishima, K., Baba, A., Matsuo, M., Itoh, Y., Oishi, R. Protective effect of cyclic AMP against cisplatin-induced nephrotoxicity. Free Radic. Biol. Med. 40: 1564-1577, 2006. https://doi.org/10.1016/j.freeradbiomed.2005.12.025
  41. Brozovic, A., Fritz, G., Christmann, M., Zisowsky, J., Jaehde, U., Osmak, M., Kaina, B. Long-term activation of SAPK/JNK, p38 kinase and fas-L expression by cisplatin is attenuated in human carcinoma cells that acquired drug resistance. Int. J. Cancer 112: 974-985, 2004. https://doi.org/10.1002/ijc.20522
  42. Ikeda, K., Kajiwara, K., Tanabe, E., Tokumaru, S., Kishida, E., Masuzawa, Y., et al. Involvement of hydrogen peroxide and hydroxyl radical in chemically induced apoptosis of HL-60 cells. Biochem. Pharmacol. 57: 1361-1365, 1999. https://doi.org/10.1016/S0006-2952(99)00055-6
  43. Wang, X., Martindale, J.L., Holbrook, N.J. Requirement for ERK activation in cisplatin-induced apoptosis. J. Biol. Chem. 275: 39435-39443, 2000. https://doi.org/10.1074/jbc.M004583200
  44. Laptenko, O., Prives, C. Transcriptional regulation by p53: one protein, many possibilities. Cell Death Differ. 13: 951-961, 2006. https://doi.org/10.1038/sj.cdd.4401916
  45. Xu, Y. Regulation of p53 responses by post-translational modifications. Cell Death Differ. 10: 400-403, 2003. https://doi.org/10.1038/sj.cdd.4401182