생명과학회지 (Journal of Life Science)

  • 제20권5호
  • /
  • Pages.760-767
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  • 2010
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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In vitro에서 항산화 효능이 있는 흑마늘 추출물의 MMP-2 및 MMP-9에 대한 활성 억제효과

In vitro Inhibitory Effect of Aged Black Garlic Extract with Antioxidant Activity on MMP-2 and MMP-9 Related to Metastasis

  • 이수진 (동의대학교 화학과) ;
  • 남향 (동의대학교 화학과) ;
  • 김문무 (동의대학교 화학과) ;
  • 장호정 (동의대학교 생명응용과학과) ;
  • 박정애 (동의대학교 생명응용과학과) ;
  • 김병우 (동의대학교 생명응용과학과) ;
  • 정경태 (동의대학교 블루바이오 소재 개발 센터)
  • Lee, Soo-Jin (Department of Chemistry, Dong-Eui University) ;
  • Nam, Hyang (Department of Chemistry, Dong-Eui University) ;
  • Kim, Moon-Moo (Department of Chemistry, Dong-Eui University) ;
  • Jang, Ho-Jung (Department of Life Science and Biotechnology, Dong-Eui University) ;
  • Park, Jung-Ae (Department of Life Science and Biotechnology, Dong-Eui University) ;
  • Kim, Byung-Woo (Department of Life Science and Biotechnology, Dong-Eui University) ;
  • Chung, Kyung-Tae (Blue-Bio Industry RIC, Dong-Eui University)
  • 투고 : 2010.04.06
  • 심사 : 2010.04.16
  • 발행 : 2010.05.31
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초록

지질, 단백질 및 DNA의 산화적 손상이 관절염, 간염, 위염, 대장염 및 치주 질환과 같은 만성 염증뿐만 아니라 암 전이에 관련되어 있다고 알려져 있다. 이러한 질환의 발생을 예방하기 위하여 독성이 없는 천연 화합물을 개발하는 것이 최근의 주요 연구 관심 대상이다. 산화적 스트레스와 관련 있는 DPPH radical, hydrogen peroxide, hydroxyl radical 및 과산화수소와 같은 활성산소에 대한 흑마늘(ABGE)의 소거능력이 연구되었다. 뿐만 아니라 TBARS assay를 이용하여 본 연구에서 사용된 산화방법으로 Fenton반응에 의하여 hydroxyl radical에 노출된 세포에서 ABGE의 항산화 효과도 조사되었다. ABGE는 활성산소종 중에서 특히 과산화수소에 대한 항산화 효능이 우수하였고 hydroxyl radical에 노출된 genomic DNA의 산화에 대한 보호 효과도 관찰되었다. 살아있는 세포에 대한 산화적 스트레스도 ABGE의 존재 하에서 억제되었다. 뿐만 아니라, 암전이와 관련 있는 MMP-2 및 MMP-9의 활성과 발현에 대한 ABGE의 효과를 gelatin zymography 및 western blot을 이용하여 조사하였다. ABGE는 PMA로 자극한 사람 섬유아육종세포로부터 분비된 MMP-2 및 MMP-9의 활성과 발현을 동시에 억제 하였으므로 암을 억제 할 수 있는 하나의 생리활성물질로 개발 될 수 있으리라 판단된다.

The oxidative damage of lipids, protein, and DNA is known to be involved in not only chronic inflammations such as arthritis, hepatitis, nephritis, gastritis, colitis, and periodontitis but also metastasis. It has given impetus to searching for natural compounds without toxicity, which prevent the development of these diseases. The direct scavenging effects of aged black garlic extract (ABGE) were evaluated in vitro on DPPH radical, hydroxyl radical, hydrogen peroxide, and genomic DNA damage related to oxidative stress. Furthermore, its antioxidant effect on lipid peroxidation was investigated in human fibrosarcoma cells (HT1080), which were exposed to the hydroxyl radical generated by the Fenton reaction. It was observed that ABGE exhibited a greater inhibitory effect on hydrogen peroxide than other reactive oxygen species, and also blocked DNA oxidation and lipid peroxidation induced by the hydroxyl radical. The oxidative stress in live cells was also inhibited in the presence of ABGE. In addition, its inhibitory effects on the activity and expression of MMP-2 and -9 related to metastasis were determined using gelatin zymography and western blot. The data showed that it inhibited MMP-2 and -9 in PMA-stimulated HT1080 cells. Therefore, these results suggest that ABGE show potential as an excellent agent for prevention of metastasis related to oxidative stress.

키워드

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