Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
권호 표지
DOI QR코드

DOI QR Code

Bioactive Materials and Biological Activity in the Extracts of Leaf, Stem Mixture and Root from Angelica gigas Nakai

참당귀 잎, 줄기혼합물과 뿌리 추출물의 생리활성물질 및 그 활성작용

  • Heo, Jin-Sun (Sancheong Institute of Medicinal Herb on Foundation) ;
  • Cha, Jae-Young (Technical Research Institute, Daesun Distilling Co., Ltd.) ;
  • Kim, Hyun-Woo (Technical Research Institute, Daesun Distilling Co., Ltd.) ;
  • Ahn, Hee-Young (Department of Medical Biosciences, Graduate School, Dong-A University) ;
  • Eom, Kyung-Eun (Department of Medical Biosciences, Graduate School, Dong-A University) ;
  • Heo, Su-Jin (Department of Medical Biosciences, Graduate School, Dong-A University) ;
  • Cho, Young-Su (Department of Biotechnology, Dong-A University)
  • 허진선 ((재)산청한방약초연구소) ;
  • 차재영 (대선주조(주) 기술연구소) ;
  • 김현우 (대선주조(주) 기술연구소) ;
  • 안희영 (동아대학교 대학원 의생명과학과) ;
  • 엄경은 (동아대학교 대학원 의생명과학과) ;
  • 허수진 (동아대학교 대학원 의생명과학과) ;
  • 조영수 (동아대학교 생명공학과)
  • Received : 2010.04.01
  • Accepted : 2010.05.10
  • Published : 2010.05.31
Download PDF
⟨ Previous Next ⟩

Abstract

The bioactive materials (phenolic compounds, flavonoids, minerals, decursin and decursinol angelate) and biological activities (DPPH [$\alpha,\alpha$'-diphenyl-$\beta$-picrylhydrazyl] free radical scavenging capability, reducing power, and tyrosinase activity) in the extracts of leaf, stem mixture (AGLS), and root (AGR) from Angelica gigas Nakai were examined by using water, hot water and ethanol solvent. The highest extract yield (21.89%) was found in the water extract of AGR. The highest concentrations of phenolic compounds and flavonoids in the ethanol extracts of AGLS and AGR were 14.99% and 14.79%. Major minerals of AGLS and AGR were K, Mg, Fe, Na and Ca. Decursin and decursinol angelate were the major ingredients of Angelica gigas, detected at 18.71 and 18.89 min of retention time by HPLC analysis, respectively. The highest concentrations of decursin and decursinol angelate in the Angelica gigas ethanol extract were found in root ($41.7\;{\mu}g/g$) and leaf ($34.04\;{\mu}g/g$). The highest free radical scavenging activity was found in the hot water extracts of AGLS and AGR, and its activity was stronger in all extracts of AGLS than AGR. The highest reducing power was found in the ethanol extracts of AGLS and AGR and this was dependent on the sample concentration. The hot water extracts of AGLS and AGR revealed the highest inhibition activity on tyrosinase. Overall, these results may provide the basic data needed to understand the biological activities of bioactive materials derived from Angelica gigas.

지리산 청정지역에서 재배되고 있는 참당귀의 생리활성 물질과 효능을 이용한 건강 기능성 식품 또는 화장품 소재 개발을 위한 기초연구의 일환으로 참당귀의 잎, 줄기 혼합물 및 뿌리의 추출방법에 따른 phenolic compounds, flavonoids, minerals, decursin 및 decursinol angelate 등의 생리활성 물질 분석과 항산화 활성 및 환원력을 측정하였다. 당귀 뿌리의 온수 추출물 수율은 21.89%로 가장 높았다. 페놀성 화합물 및 플라보노이드 함량은 참당귀 잎, 줄기 혼합물의 에탄올 추출물에서 가장 높은 함량을 보였고, 참당귀 뿌리 보다는 잎, 줄기 혼합물에서 높았고, 수용성 추출물보다 에탄올 추출물에서 높았다. 미네랄함량은 참당귀 잎, 줄기 혼합물 및 뿌리 모두에서 K, Mg, Fe, Na, Ca, Mn, Zn 순으로 함유되어 있었다. HPLC 분석에 의한 참당귀 잎, 줄기, 뿌리의 decursin 및 decursinol angelate의 혼합 면적 비율은 각각 66.1%, 82.2%, 79.8%로 나타났으며, decursin은 13.0%, 37.6%, 47.1%로 뿌리에서 가장 높았고 decursinol angelate는 53.2%, 44.6%, 32.6%로서 잎에서 가장 높았다. Free radical scavenging 항산화활성은 참당귀 잎, 줄기 혼합물 및 뿌리 각각의 열수 추출물에서 가장 높았으며, 뿌리보다는 잎, 줄기 혼합물의 모든 추출물에서 항산화 활성이 높았다. Fe 및 Cu 환원력은 참당귀 잎, 줄기 혼합물 및 뿌리의 온수, 열수 및 에탄올 추출물 순으로 높게 나타났으며, 참당귀 잎, 줄기 혼합물의 추출물이 뿌리 추출물 보다 높은 활성을 보였는데 이는 페놀성 화합물이나 플라보노이드 함량과 밀접한 관계를 보였다. Tyrosinase 저해 활성은 참당귀 잎, 줄기 혼합물 및 뿌리의 열수 추출물에서 가장 높은 것으로 나타났다. 이상의 실험 결과 참당귀에탄올 추출물에서는 페놀성 화합물과 플라보노이드 함량 및 환원력이 높았으며, 열수 추출물에서는 항산화 효과가 높아 참당귀 유래의 생리활성물질과 그 활성을 이용한 제품 개발에 필요한 기초 자료를 제공해주는데 유용하게 사용될 수 있을 것으로 생각된다.

Keywords

References

  1. Abe, N., T. Murata, and A. Hirota. 1998. Novel DPPH radical scavengers, bisorbicillinol and demethyltrichodimerol, from a fungus. Biosci. Biotechnol. Biochem. 62, 661-666. https://doi.org/10.1271/bbb.62.661
  2. AOAC. 1975. Official methods of analysis. 12th ed., Association of official analytical chemists. Washington, D.C., USA.
  3. Cabanes, J., S. Chazarra, and F. Garcia-Carmona. 1994. Kojic acid, a cosmetic skin whitening agent, is a slow binding inhibitor of catecholase activity of tyrosinase. J. Pharm. Pharmacol. 46, 982-985. https://doi.org/10.1111/j.2042-7158.1994.tb03253.x
  4. Cha, J. Y., H. J. Kim, C. H. Chung, and Y. S. Cho. 1999. Antioxidative activities and contents of polyphenolic compound of Cudrania tricuspidata. J. Korean Soc. Food Sci. Nutr. 28, 1310-1315.
  5. Cha, J. Y., Y. S. Cho, I. Kim, T. Anno, S. M. Rahman, and T. Yanagita. 2001. Effect of hesperetin, a citrus flavonoid, on the liver triacylglycerol content and phosphatidate phosphohydrolase activity in orotic acid-fed rats. Plant Foods Human Nutr. 56, 349-358. https://doi.org/10.1023/A:1011884200848
  6. Cha, J. Y., H. Y. Ahn, K. E. Eom, B. K. Park, B. S. Jun, and Y. S. Cho. 2009. Antioxidative activity of Aralia elata shoot and leaf extracts. J. Life Sci. 19, 652-658. https://doi.org/10.5352/JLS.2009.19.5.652
  7. Duncan, D. B. 1959. Multiple range and multiple F test. Biometrics 1, 1-42. https://doi.org/10.1002/bimj.19590010102
  8. Han, G. J., D. S. Shin, and M. S. Jang. 2008. A study of the nutritional composition of Aralica continentalis Kitagawa and Aralica continentalis Kitagawa leaf. Korean J. Food Sci. Technol. 40, 680-685.
  9. Hong, M. W. 1972. Statistical studies on the formularies of oriental medicine(I) prescription frequency and their origin distribution of herb drugs. Korean J. Pharmaco. 3, 57-64.
  10. Hwang, J. B. and M. O. Yang. 1997. Comparison of chemical components of Angelica gigas Nakai and Angelica acutiloba Kitagawa. Korean J. Food Sci. Technol. 29, 1113-1118.
  11. Jayaprakasha, G. K., R. P. Singh, and K. K. Sakariah. 2001. Antioxidant activity of grape seed (Vitis vinifera) extracts on peroxidation models in vitro. Food Chem. 73, 285-290. https://doi.org/10.1016/S0308-8146(00)00298-3
  12. Jeong, H. S., J. G. Han, J. H. Ha, Y. Kim, S. H. Oh, S. S. Kim, M. H. Jeong. G. P. Choi, W. Y. Park, and H. Y. Lee. 2009. Enhancement of anticancer activities ofEphedra sinica, Angelica gigas by ultra high pressure extraction. Korean J. Medicinal Crop Sci. 17, 102-108.
  13. Jia, Z., M. Tang, and J. Wu. 1999. The determination of flavonoid contents in mulberry and thier scavenging effects on superoxide radicals. Food Chem. 64, 555-559. https://doi.org/10.1016/S0308-8146(98)00102-2
  14. Johnson, J. E., R. Walford, D. Harma, and J. Miquel. 1986. In 'Free radicals, aging and degenerative disease', Alen R. Liss, N.Y.
  15. Jung, S. W., N. K. Lee, S. J. Kim, and D. S. Han. 1995. Screening of tyrosinase inhibitor from plants. Korean J. Food Sci. Technol. 27, 891-896.
  16. Jung, W. S., C. Y. Yu, J. G. Park, M. J. Kim, J. H. Kim, and J. K. Kim. 2006. Comparison of biological activities in extracts from Oplopanax elatus. Korean J. Medicinal Crop. Sci. 14, 630-631.
  17. Kim, C. H., M. C. Kwon, H. G. Han, C. S. Na, H. G. Kwak, G. P. Choi, U. Y. Park, and H. Y. Lee. 2008. Skin-whitening and UV-protective effects of Angelicagigas Nakai extracts on ultra high pressure extraction process. Korean J. Medicinal Crop. Sci. 16, 255-260.
  18. Kim, E. Y., I. H. Baik, J. H. Kim, S. R. Kim, and M. R. Rhyu. 2004. Screening of the antioxidant activity of some medicinal plants. Korean J. Food Sci. Technol. 36, 333-338.
  19. Kim, H. S. and S. W. Joung. 2006. Effective components and nitrile scavenging ability of root and leaves a Angelica gigas Nakai. Korean J. Food Cookery Sci. 22, 957-965.
  20. Kim, K. M., J. Y. Jung, S. W. Hwang, M. J. Kim, and J. S. Kang. 2009. Isolation and purification of decursin and decursinol angelate in Angelica gigas Nakai. J. Korean Soc. Food Sci. Nutr. 38, 653-656. https://doi.org/10.3746/jkfn.2009.38.5.653
  21. Kim, S. B., Y. H. Kim, C. W. Lee, S. M. Park, K. S. Ahn, I. H. Kim, and H. M. Kim. 1998. Characteristic immunostimulation by angelan isolated from Angelica gigas Nakai. Immunopharmacol. 40, 39-48. https://doi.org/10.1016/S0162-3109(98)00026-5
  22. Kim, S. J., M. Y. Heo, K. H. Bae, S. S. Kang, and H. P. Kim. 2003. Tyrosinase inhibitory activity of plant extract (III): Fifty Korean indigenous plants. J. Applied Phamacol. 11, 245-248.
  23. Lee, J. H. and S. R. Lee. 1994. Analysis of phenolic substances content on Korea plant foods. Korean J. Food Sci. Technol. 26, 310-316.
  24. Lee, J. J., A. R. Kim, Y. N. Seo, and M. Y. Lee. 2009. Comparison of physicochemical composition of three species of genus Angelica. Korean J. Food Preserv. 16, 94-100.
  25. Lee, S. O., H. J. Lee, M. H. Yu, H. G. Im, and I. S. Lee. 2005. Total polyphenol contents and antioxidant activities of methanol extracts from vegetables produced in Ullung Island. Korean J. Food Sci. Technol. 37, 233-243.
  26. Lee, Y. Y., S. Lee, J. L. Jin, and H. S. Yun-Choi. 2003. Platelet anti-aggregatory effects of coumarins from the roots of Angelica genuflexa and A. gigas. Arch Pharm. Res. 26, 723-726. https://doi.org/10.1007/BF02976681
  27. Luthringer, C. Y. Rayssiguier, E. Gueux, and A. Berthelot. 1988. Effect of moderate magnesium deficiency on serum lipids, blood pressure and cardiovascular reactivity in normotensive rats. Br. J. Nutr. 59, 243-250. https://doi.org/10.1079/BJN19880031
  28. Maxson, E. and L. Rooney. 1972. Evaluation of methods for tannin analysis in sorghum grain. Cereal Chem. 49, 719-729.
  29. Masamoto, Y., H. Ando, Y. Murata, Y. Shimoishi, M. Tada, and K. Takahata. 2003. Mushroom tyrosinase inhibitory activity of esculetin isolated from seeds ofEuphorbia lathyris L. Biosci. Biotechnol. Biochem. 67, 631-634. https://doi.org/10.1271/bbb.67.631
  30. Oh, S. H., Y. S. Cha, and D. S. Choi. 1999. Effects of Angelica gigas Nakai diet on lipid metabolism, alcohol metabolism and liver function of rats administered with chronic ethanol. J. Korean Soc. Agric. Chem. Biotechnol. 42, 29-33.
  31. Oh, S. L., S. S. Kim, B. Y. Min, and D. H. Chung. 1990. Composition of free sugars, free amino acids, non-volatile organic acids and tannins in the extracts of L. chinensis M., A. acutiloba K., S. chinesis B. and A. sessiliflorum S. Korean J. Food Sci. Technol. 22, 76-81.
  32. Park, K. W., S. R. Choi, M. E. Shon, I. Y. Jeong, K. S. Kang, S. T. Lee, K. H. Shim, and K. I. Seo. 2007. Cytotoxic effects of decursin from Angelica gigas Nakai in human cancer cells. J. Korean Soc. Food Sci. Nutr. 36, 1385-1390. https://doi.org/10.3746/jkfn.2007.36.11.1385
  33. Ryu, K. S., N, D, Hong, and Y. Y. Kim. 1990. Studies on the coumarin constituents of the root of Angelica gigas Nakai. Isolation of decursinol angelate and assay of decursinol angelate and decursin. Korean J. Pharmaco. 21, 64-68.
  34. Seong, N. S., S. W. Lee, K. S. Kim, and S. T. Lee. 1993. Environmental variation of decursin content in Angelica gigas. Korean J. Crop Sci. 38, 60-65.
  35. Shon, M. E. 2007. Antioxidant and anticancer activities of Poria cocos and Machilus thunbergii fermented with mycelial mushrooms. Food Indus. Nutr. 12, 51-57.
  36. Swain, T., W. E. Hillis, and M. Oritega. 1959. Phenolic constituents of Ptunus domestica. I. Quantitative analysis of phenolic constituents. J. Sci. Food Agric. 10, 83-88.
  37. Vile, G. F. and R. M. Tyrrell. 1995. UVA radiation-induced oxidative damage to lipid and protein in vitro and in human skin fibroblast is dependent on iron and singlet oxygen. Free Radical Biol. Med. 18, 721-730. https://doi.org/10.1016/0891-5849(94)00192-M
  38. Zhu, Q. V., R. M. Hackman, X. X. Jodilensunsa, R. R. Holt, and C. L. Keen. 2002. Antioxidative activities of Oolong tea. J. Agric. Food Chem. 50, 6229-6934.

Cited by

  1. Effect of extract temperature and duration on antioxidant activity and sensory characteristics of Ulmus pumila bark extract vol.23, pp.7, 2016, https://doi.org/10.11002/kjfp.2016.23.7.995
  2. Effects of Angelica gigas extract on the oral pharmacokinetics of gefitinib in rats 2017, https://doi.org/10.1007/s40005-017-0315-y
  3. Effect of Fermented Angelica gigas Nakai on Lipid Metabolism in Orotic Acid Model Rats vol.24, pp.7, 2014, https://doi.org/10.5352/JLS.2014.24.7.743
  4. Antiobesity activity of fermentedAngelicae gigantisby high fat diet-induced obese rats 2011, https://doi.org/10.3109/14756366.2011.615746
  5. Effects of Monascus-fermented Angelica gigas Nakai on the Contents of Serum Lipid and Tissue Lipid Peroxidation in Alcohol Feeding Rats vol.23, pp.11, 2013, https://doi.org/10.5352/JLS.2013.23.11.1371
  6. The Improvement Effect of Fermented Angelica gigas Nakai Powder Against Orotic Acid-induced Fatty Liver in Rats vol.24, pp.4, 2014, https://doi.org/10.5352/JLS.2014.24.4.411
  7. Biological Activities of Extracts from Flowers of Angelica gigas Nakai vol.40, pp.8, 2011, https://doi.org/10.3746/jkfn.2011.40.8.1079
  8. Biological Activity of Methanolic Extract from Ganoderma lucidum, Momordica charantia, Fagopyrum tataricum, and Their Mixtures vol.21, pp.7, 2011, https://doi.org/10.5352/JLS.2011.21.7.1016