생명과학회지 (Journal of Life Science)

  • 제20권5호
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  • Pages.697-702
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  • 2010
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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돼지에서 브루셀라병 항체조사 및 Yersinia enterocolitica O:9의 분리

Seroprevalence of Brucellosis and Isolation of Yersinia enterocolitica O:9 in Pigs

  • 정병열 (국립수의과학검역원 질병진단센터) ;
  • 변재원 (국립수의과학검역원 질병진단센터) ;
  • 김하영 (국립수의과학검역원 질병진단센터) ;
  • 신동호 (국립수의과학검역원 질병진단센터) ;
  • 박최규 (국립수의과학검역원 질병진단센터) ;
  • 정석찬 (국립수의과학검역원 세균과)
  • Jung, Byeong-Yeal (Animal Disease Diagnostic Center, National Veterinary Research and Quarantine Service) ;
  • Byun, Jae-Won (Animal Disease Diagnostic Center, National Veterinary Research and Quarantine Service) ;
  • Kim, Ha-Young (Animal Disease Diagnostic Center, National Veterinary Research and Quarantine Service) ;
  • Shin, Dong-Ho (Animal Disease Diagnostic Center, National Veterinary Research and Quarantine Service) ;
  • Park, Choi-Kyu (Animal Disease Diagnostic Center, National Veterinary Research and Quarantine Service) ;
  • Jung, Suk-Chan (Bacteriology and Parasitology Division, National Veterinary Research and Quarantine Service)
  • 투고 : 2010.01.13
  • 심사 : 2010.03.03
  • 발행 : 2010.05.31
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초록

2007년 전국 10개의 일반 양돈장에서 다양한 일령의 돼지 578두를 채혈하여 Rose Bengal test 진단액으로 스크리닝을 하고, 양성 혈청에 대해서는 시험관응집반응과 C-ELISA를 적용하여 브루셀라병 항체 분포도 양상과 브루셀라 교차반응 유발균의 분리를 시도하여 다음과 같은 결론을 얻었다. 돼지에서 브루셀라병 Rose Bengal 스크리닝 결과, 10주령 미만(양성률; 14.6%, 95% CI; 10.1-19.1)자돈보다 10주령에서 17주령 미만(양성률; 31.3%, 95% CI; 22.8-39.8)과 17주령 이상(양성률; 30.4%, 95% CI; 34.3-36.4)의 돼지에서 양성률이 유의성 있게 높았다(p<0.05). 따라서 검사돈 중 139두 (24.0%)에서 Rose Bengal test 양성반응이 나타났다. 양돈장별 Rose Bengal test 양성률 조사에서 6개 양돈장이 10% 미만의 양성률을 보였고, 2개 양돈장에서는 70% 이상의 높은 양성률이 관찰되었다. Rose Bengal test 양성 혈청 139건을 시험관응집반응과 C-ELISA에 적용한 결과, 시험관응집반응에서 100배 이상의 양성이 48건(34.5%)이었으나 C-ELISA에서는 전 두수 음성으로 나타나 돼지 혈청에 대해서는 시험관응집반응 성적과 C-ELISA 성적에 많은 차이가 있었다. 시험관응집반응에서 높은 항체가를 보인 돼지의 직장 swab 시료(n=26)에서 브루셀라 균과 교차반응을 유발하는 균종에 대해 균분리를 실시한 결과 E. coli O157:H7, Salmonella N group은 분리되지 않았으나 Y. enterocolitica O:9은 7건(26.9%)이 분리되었다.

Ten farrow-finish farms participated in this seromonitoring that was conducted to investigate the porcine brucellosis situation in Korea. In total, eight (80.0%) of the 10 farms and 139 (24.0%) of 578 pigs tested showed a positive response in the Rose Bengal test (RBT). Seroprevalence levels were determined using RBT according to age; 35 (14.6%) of 239 piglets, 36 (31.3%) of 115 growing pigs, and 68 (30.4%) of 224 finishing pigs and sows were positive, respectively. All positive samples in RBT were tested with the tube agglutination test (TAT) and competitive ELISA (C-ELISA), simultaneously. Although 48 samples came up positive in the TAT, all samples tested with C-ELISA were negative. Among 26 rectal swab samples from the TAT positive-pigs, Yersinia enterocolitica O:9 was isolated from seven samples (26.9%). Therefore, we speculated that the positive reaction of RBT and TAT in this study might be induced by the serologically cross-reacting bacteria with Brucella abortus.

키워드

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