생명과학회지 (Journal of Life Science)

  • 제20권5호
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  • Pages.670-675
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  • 2010
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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Autoimmune regulator gene (Aire)의 마우스 림프절 FRC세포, BLS4에서 발현

Autoimmune Regulator Gene (Aire) is Expressed in Lymph Node Fibroblastic Reticular Cell, BLS4

  • 문경미 (동의대학교 바이오물질제어학과) ;
  • 서희주 (동의대학교 생명공학과) ;
  • 오지윤 (동의대학교 생명공학과) ;
  • 이재설 (동의대학교 생명공학과) ;
  • 김해영 (동의대학교 생명공학과) ;
  • 김정환 (동의대학교 바이오물질제어학과) ;
  • 최우봉 (동의대학교 바이오물질제어학과) ;
  • 김병우 (동의대학교 블루바이오 RIC) ;
  • 김광현 (동의대학교 생명응용학과) ;
  • 이종환 (동의대학교 바이오물질제어학과)
  • Moon, Kyoung-Mi (Department of Biomaterial Control, Dong-Eui University) ;
  • Seo, Hee-Ju (Department of Biotechnology and Bioengineering, Dong-Eui University) ;
  • Oh, Ji-Youn (Department of Biotechnology and Bioengineering, Dong-Eui University) ;
  • Lee, Jae-Seol (Department of Biotechnology and Bioengineering, Dong-Eui University) ;
  • Kim, Hae-Young (Department of Biotechnology and Bioengineering, Dong-Eui University) ;
  • Kim, Jeong-Hwan (Department of Biomaterial Control, Dong-Eui University) ;
  • Choi, Woo-Bong (Department of Biomaterial Control, Dong-Eui University) ;
  • Kim, Byung-Woo (Department of Control Blue-Bio Regional Innovation Center, Dong-Eui University) ;
  • Kim, Kwang-Hyeon (Department of Life Science and Biotechnology, Dong-Eui University) ;
  • Lee, Jong-Hwan (Department of Biomaterial Control, Dong-Eui University)
  • 투고 : 2010.03.11
  • 심사 : 2010.05.24
  • 발행 : 2010.05.31
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초록

Autoimmune regulator gene (Aire)는 흉선에서 발현되며 promiscuous genes으로 알려진 흉선에서 자가항원 발현을 조절한다. Aire 와 promiscuous genes은 흉선에서 T세포 tolerance와 자가면역에 관여한다. 말초 조직 즉 림프절에서 Aire의 역할을 알아보고자 림프절 구성 세포중 하나인 fibroblastic reticular cell (FRC)을 분리 확립하였다. 마우스 림프절로부터 분리된 FRC에서 Aire의 발현을 확인하였고 또한 promiscuous antigen인 insulin의 발현도 확인하였다. Aire 과발현 플라스미드로 형질전환 후 배양 FRC에서 Insulin의 발현이 증가하였다. 이것은 Aire가 FRC에서 promiscuous gene의 발현을 조절한다는 것을 보여주며 peripheral selection과 연관되어 있을 수 있다는 것을 제시한다.

Autoimmune regulator gene (Aire) is expressed in the thymus and controls the expression of peripheral self-antigens, known as promiscuous genes. Aire and promiscuous genes are involved in T cell tolerance and autoimmunity in the thymus. Here, we identified Aire-expressing fibroblastic reticular cell (FRC), which was derived from mouse lymph node and also expressed in insulin promiscuous antigen. The expression of insulin was increased in cultured FRC over-expressed with Aire. These data suggest that Aire regulates promiscuous gene expression in FRC, and that this function might be under peripheral selection control.

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참고문헌

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