Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Multiplex PCR-aided Differential Diagnosis of Taeniid Species

Multiplex PCR을 이용한 조충류의 감별진단

  • Lee, Hye-Jung (Department of Parasitology, College of Medicine, Dankook University) ;
  • Seo, Min (Department of Parasitology, College of Medicine, Dankook University) ;
  • Kwak, Sahng-June (Department of Biochemistry, College of Medicine, Dankook University)
  • 이혜정 (단국대학교 의과대학 기생충학교실) ;
  • 서민 (단국대학교 의과대학 기생충학교실) ;
  • 곽상준 (단국대학교 의과대학 생화학교실)
  • Received : 2010.05.13
  • Accepted : 2010.06.08
  • Published : 2010.06.30
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Abstract

Differential diagnosis of the taeniid proglottids between Taenia asiatica and T. sagniata is a daunting task due to their close morphological similarity. However, to correctly diagnose them on time is important in managing infected patients, as well as for reducing serious complications such as cysticercosis. Currently, DNA-based methods for the dissection of genomic information of parasites are being employed to make accurate and rapid diagnoses in the field of parasitology. In this study, multiplex PCR was established and exploited to identify exact species of taeniid adult worms recovered from Korean people. To discriminate one from the other other, primers-Ta4978F, Ts5058F, Tso7421F, and Rev7915- were used for the multiplex PCR, which provided swift and precise identification of the taeniid worms being observed. Also,having instituted PCR methodology, we ascertained that easiness would be achieved to reassess and re-evaluate Korean endemic data on human taeniid cestodes.

아시아조충과 무구조충의 편절은 형태학적으로 유사해 감별진단하기가 쉽지 않다. 하지만 아시아조충의 경우 감염자에서 낭미충증 등의 심각한 합병증을 일으킬 가능성을 배제할 수 없으므로 두 기생충의 정확한 진단이 필요하다. 최근 기생충학 분야에서 DNA 서열에 기초한 진단 방법이 널리 쓰이고 있다. 본 연구에서는 다중 중합 효소연쇄반응을 이용해 한국인에서 발견된 태니아 속 조충류의 감별진단을 시도해 보고자 했다. 중합효소연쇄반응을 위해 Ta4978F, Ts5058F, Tso7421F, Rev7915 4개의 시발체(프라이머)를 사용했으며, 그 결과 태니아의 종 동정이 정확하고 신속하게 이루어질 수 있었다. 중합효소연쇄반응 방법을 도입한다면 한국에서 인체 태니아 조충의 역학적 소견을 용이하게 재검토할 수 있으리라고 생각한다.

Keywords

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