Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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A Study on the Prolactin Receptor 3 (PRLR3) Gene and the Retinol-binding Protein 4 (RBP4) Gene as Candidate Genes for Growth and Litter Size Traits of Berkshire in Korea

국내 버크셔 돼지에서 성장 및 산자수의 후보유전자로서 PRLR3와 RBP4에 관한 연구

  • Do, Chang-Hee (Department of Animal Biosystem Science, Chungnam National University) ;
  • Kim, Seon-Ku (Department of Animal Science, Pusan National University) ;
  • Kang, Han-Suk (Department of Animal Science, Pusan National University) ;
  • Shin, Teak-Soon (Department of Animal Science, Pusan National University) ;
  • Lee, Hong-Gu (Department of Animal Science, Pusan National University) ;
  • Cho, Seong-Keun (Department of Animal Science, Pusan National University) ;
  • Do, Kyung-Tak (Department of Animal Science, Pusan National University) ;
  • Song, Ji-Na (Department of Animal Science, Pusan National University) ;
  • Kim, Tae-Hun (Animal Genomics and Bioinformatics Division, National Livestock Research Institute, RDA) ;
  • Choi, Bong-Hwan (Animal Genomics and Bioinformatics Division, National Livestock Research Institute, RDA) ;
  • Sang, Byung-Chan (Department of Animal Biosystem Science, Chungnam National University) ;
  • Joo, Yeong-Kuk (Gyeongnam Livestock Veterinary Research Institute) ;
  • Park, Jun-Kyu (Gyeongnam Livestock Veterinary Research Institute) ;
  • Lee, Sung-Hoon (Gyeongnam Livestock Veterinary Research Institute) ;
  • Lee, Jeong-Ill (Gyeongnam Livestock Veterinary Research Institute) ;
  • Park, Jeong-Suk (Gyeongnam Livestock Veterinary Research Institute) ;
  • Sin, Young-Soo (Department of Animal Science, Shingu University) ;
  • Kim, Byung-Woo (Institute of Agriculture & Life Sciences, Gyeongsang University) ;
  • Cho, Byung-Wook (Department of Animal Science, Pusan National University)
  • 도창희 (충남대학교 동물바이오시스템과학과) ;
  • 김선구 (부산대학교 동물생명자원과학과) ;
  • 강한석 (부산대학교 동물생명자원과학과) ;
  • 신택순 (부산대학교 동물생명자원과학과) ;
  • 이홍구 (부산대학교 동물생명자원과학과) ;
  • 조성근 (부산대학교 동물생명자원과학과) ;
  • 도경탁 (부산대학교 동물생명자원과학과) ;
  • 송지나 (부산대학교 동물생명자원과학과) ;
  • 김태헌 (축산과학원 동물유전체과) ;
  • 최봉환 (축산과학원 동물유전체과) ;
  • 상병찬 (충남대학교 동물바이오시스템과학과) ;
  • 주영국 (경남축산진흥연구소) ;
  • 박준규 (경남축산진흥연구소) ;
  • 이성훈 (경남축산진흥연구소) ;
  • 이정일 (경남축산진흥연구소) ;
  • 박정석 (경남축산진흥연구소) ;
  • 신영수 (신구대학교 자원동물과) ;
  • 김병우 (경상대학교 농업생명과학연구원) ;
  • 조병욱 (부산대학교 동물생명자원과학과)
  • Received : 2010.02.22
  • Accepted : 2010.05.07
  • Published : 2010.06.30
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Abstract

Two diallelic markers at candidate gene loci, the prolactin receptor 3 (PRLR3) gene and the retinol-binding protein 4 (RBP4) gene were evaluated for their association with growth and litter size traits in Berkshire. Genetic evaluation was conducted for 5,919 pigs with pedigree information, which included 3,480 growth performance records and 775 litter size records of 224 sows. From the same herd, genotyping was carried out on 144 and 156 animals for PRLR3 and RBP4, respectively. After assigning a genotype to subjects in which both parents had a homozygous genotype, numbers of genotyped animals increased to 474 and 338, for the PRLR3 gene and RBP4 gene, respectively. The genotype effects of two markers were estimated with breeding values of the genotyped animals. The additive effects of total number of piglets born and number of piglets born alive in the PRLR3 locus were -0.28 and -0.13, respectively. The dominance effect of the RBP4 locus on average daily gain was -10.58 g. However, the polymorphism of the RBP4 locus in total number of piglets born and number of piglets born alive has shown -0.34 and -0.33 of the additive genetic effects. In view of the results, MAS (marker-assisted selection) favoring B alleles of RBP4 and PRLR3 loci could potentially accelerate the rate of the genetic improvement in the litter size traits.

본 연구는 버크셔 품종에서 PRLR3와 RBP4 후보유전자의 두 개의 대립유전자가 산육형질과 번식형질에 미치는 영향을 조사하였다. 5,919두의 혈통자료, 3,480두의 산육기록과, 244두의 모돈의 775마리의 산자기록을 이용하여 유전능력 평가를 수행하였다. 유전자형 분석은 144두와 156두에서 PRLR3와 RBP4 유전자의 유전자형을 각각 분석하였다. 평가된 개체들의 육종가를 이용 두 마커의 유전자형 효과와 유의 확률을 추정한 결과 PRLR3 유전자는 번식형질의 총산자수(TBN)와 생존산자수(NBA)에서 -0.28과 -0.13두의 상가적 효과를 각각 나타내었다. RBP4 유전자는 일당증체량에서 10.58 g의 우성적 효과를 나타내었다. 그러나 RBP4 유전자의 다형성은 번식형질의 총 산자수(TBN)와 생존산자수(NBA)에서 -0.34와 -0.33두의 상가적 유전적 효과를 각각 나타났다. 따라서 PRLR3와 RBP의 B 대립유전자를 선호하는 MAS (Marker Assist Selection) Scheme은 버크셔 품종의 산자수의 개량에 이용 할 수 있을 것이다.

Keywords

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