생명과학회지 (Journal of Life Science)

  • 제20권7호
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  • Pages.1143-1150
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  • 2010
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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한약재 추출물의 피부 염증 유발 세균에 대한 항균활성

Antibacterial Activity of Oriental Medicinal Herb Extracts against Skin Pathogens

  • 유영은 (대구한의대학교 한방생명자원연구센터) ;
  • 박은영 (대구한의대학교 한방생명자원연구센터) ;
  • 정대화 (대구한의대학교 한방생명자원연구센터) ;
  • 변성희 (대구한의대학교 한의학과) ;
  • 김상찬 (대구한의대학교 한방생명자원연구센터) ;
  • 박성민 (대구한의대학교 한방생명자원연구센터)
  • Yu, Young-Eun (The Research Center for Biomedical Resources of Oriental Medicine, Daegu Haany University) ;
  • Park, Eun-Young (The Research Center for Biomedical Resources of Oriental Medicine, Daegu Haany University) ;
  • Jung, Dae-Hwa (The Research Center for Biomedical Resources of Oriental Medicine, Daegu Haany University) ;
  • Byun, Sung-Hui (College of Oriental Medicine, Daegu Haany University) ;
  • Kim, Sang-Chan (The Research Center for Biomedical Resources of Oriental Medicine, Daegu Haany University) ;
  • Park, Sung-Min (The Research Center for Biomedical Resources of Oriental Medicine, Daegu Haany University)
  • 투고 : 2010.05.17
  • 심사 : 2010.06.17
  • 발행 : 2010.07.30
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초록

한약재 70종의 메탄올 추출물을 이용하여 4종의 Staphylococcus epidermidis와 S. aureus에 대한 항균효과를 조사한 결과 모든 공시균주에 대하여 항균성을 나타내는 한약재는 황련, 소목, 오배자, 대황과 단삼으로 조사되었다. 이 중 우수한 항균성을 나타낸 오배자와 황련을 물, 에탄올, 메탄올과 에틸아세테이트를 추출 용매로 하여 추출물을 제조하고 항균성을 조사한 결과 황련 열수 추출물에서 가장 우수한 항균성을 확인할 수 있었다. 황련의 항균성은 berberine에 의한 것으로 가정하고 열수 추출물 중 berberine의 함량을 조사한 결과 13.88%로 조사되었다. Berberine에 의한 항균성과 다른 항생제와의 항균성을 비교하기 위하여 berberine chloride와 3종의 항생제를 이용하여 조사한 결과 600 mg/l 이상의 berberine chloride 농도에서 KCCM 35494 S. epidermidis에 대한 항균성만 나타내었으며 다른 공시균주에 대한 항균성은 확인되지 않았다. 이에 본 연구에 사용한 공시균주에 대한 C. japonica 열수 추출물의 항균성은 고 농도의 berberine 또는 다른 물질에 의한 것으로 판단할 수 있었으며 이 물질에 대한 연구가 필요할 것으로 판단되었다.

The antibacterial activity of methanol extracts from 70 kinds of oriental medicinal herbs on four strains of Staphylococcus aureus and S. epidermidis was investigated. The results showed that C. japonica, C. sappan, R. javanica, R. tanguticum, and S. miltiorrhiza had an antibacterial activity on all the strains used. Among these, C. japonica and R. javanica, which showed excellent antibacterial activity, were extracted with water, ethanol, methanol, and ethyl acetate for further study of antibacterial activities. The results showed that the boiled water extract of C. japonica had the best antibacterial activity. Assuming that the antibacterial activity of C. japonica originated from berberine, which has been reported many times, the berberine content of the boiled water extract of C. japonica was analyzed and the result was 13.88%. To compare the antibacterial activity of berberine with that of other antibiotics, berberine chloride and three other kinds of antibiotics were investigated, which showed that berberine chloride had an antibacterial activity on KCCM 35494 S. epidermidis only when the concentration was higher than 600 mg/l, and it did not show antibacterial activity in the other strains. Based on these results, it was concluded that the antibacterial activity of the boiled water extract of C. japonica on the strains used in this study originated from high concentration of berberine or substances other than berberine. Therefore, identification of the substance will be necessary.

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