Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Protective Effect of Prunella spica Extracts against H2O2-Induced Cytotoxicity in PC12 Cells

Hydrogen peroxide가 유도하는 세포독성으로부터 PC12 세포를 보호하는 하고초(Prunella spica) 추출물의 영향

  • Kim, Hyun-Jung (Department of Food Science and Biotechnology, Kyungnam University) ;
  • Lee, Jeung-Min (Department of Food Science and Biotechnology, Kyungnam University) ;
  • Moon, Seong-Hee (Department of Food Science and Biotechnology, Kyungnam University) ;
  • Park, Hae-Ryong (Department of Food Science and Biotechnology, Kyungnam University)
  • 김현정 (경남대학교 식품생명학과) ;
  • 이정민 (경남대학교 식품생명학과) ;
  • 문성희 (경남대학교 식품생명학과) ;
  • 박해룡 (경남대학교 식품생명학과)
  • Received : 2010.05.03
  • Accepted : 2010.05.26
  • Published : 2010.07.30
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Abstract

The oxidative stress induced by reactive oxygen species (ROS) may play an important role in the pathogenesis of neurodegenerative diseases. In this study, we investigated the neuroprotective effects of methanolic extracts of Prunella Spica (PSE) against $H_2O_2$-induced oxidative stress in PC12 cells. The cells exposed to $H_2O_2$-induced oxidative stress were treated with various concentrations of PSE; this treatment resulted in the induction of a dose-dependent protective effect, which was evidenced by the results of MTT reduction assay, lactate dehydrogenase (LDH) release assay, morphological assay, and colony-formation assay. Interestingly, we also observed reduction of apoptotic bodies in the Hoechst staining and flow cytometric analysis. These data show that apoptosis was significantly suppressed in the PC12 cells that were exposed to $H_2O_2$-induced oxidative stress and treated with PSE. These results suggest that Prunella Spica could be a new potential protective agent against $H_2O_2$-induced oxidative stress.

활성산소에 의해 유도되는 산화적 스트레스가 노화와 관련된 각종 퇴행성 신경질환의 원인으로 밝혀짐에 따라 본 연구에서는 활성산소종인 $H_2O_2$가 유도하는 강력한 산화적 스트레스로부터 신경세포를 보호하는 물질을 탐색하기 위하여 하고초 추출물(PSE)에 관하여 연구하였다. PC12 세포를 이용하여 MTT reduction assay, LDH release assay 및 colony formation assay 등 여러 가지 생물학적인 assay를 통하여 PSE의 세포 보호효과를 확인하였다. 그리고 광학 현미경을 이용한 형태학적 변화 관찰에서도 $H_2O_2$ 처리군에 비해 높은 세포 보호효과를 확인할 수 있었으며, Hoechst 33342 염색과 세포주기 분석을 통하여 PSE의 높은 apoptosis 억제효과를 확인할 수 있었다. 이상의 결과로부터 하고초는 $H_2O_2$에 의해 유도된 세포독성으로부터 PC12 세포손상을 강력하게 억제하는 효과가 있다는 것을 확인할 수 있었으며, 퇴행성 신경질환에 대한 새로운 치료제로서의 가능성을 제시하였다.

Keywords

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