DOI QR코드

DOI QR Code

국산 적송수피 추출물의 브로일러에 대한 항생제 대체효과

Effect of Dietary Antibiotic Replacement with Korean Red Pine Bark Extracts in Broiler Diets

  • 박병성 (강원대학교 동물생명공학과)
  • 투고 : 2010.03.26
  • 심사 : 2010.04.29
  • 발행 : 2010.07.30

초록

본 연구는 브로일러의 성장능력, 도체특성, 혈액지질, 면역능력 및 맹장미생물 변화에 관한 국산 적송수피추출물의 항생제 대체효과를 조사하기 위하여 수행하였다. 실험처리구는 대조구, 항생제 첨가구, 적송수피추출물 65ppm 및 80 ppm 첨가구 등 4개로 구분하여 완전임의배치였다. 브로일러의 성장능력, 도체특성, 도체중, 도체율, 가슴살과 다리살 무게는 적송수피추출물 80 ppm의 첨가구가 대조구, 항생제 첨가구와 비교할 때 유의하게 높았다. 복강지방은 브로일러 사료 내 2개의 서로 다른 수준의 적송수피추출물 첨가구가 대조구 및 항생제 첨가구에 비해서 유의하게 감소하였으나, 흉선, 비장 및 F낭 등 면역기관 무게는 적송수피추출물 80 ppm 첨가구에서 유의하게 증가하였다. 브로일러의 혈액 중성지방과 총콜레스테롤은 브로일러 사료 내 2개의 서로 다른 수준의 적송수피추출물 첨가구가 대조구 및 항생제 첨가구와 비교할 때 유의하게 낮았다. 혈액 IgG는 브로일러 사료 내 적송수피추출물 80 ppm 첨가구가 대조구 및 항생제 첨가구에 비해서 유의하게 증가하였다. 브로일러 맹장 내 Bifidobacterium, Lactobacillus 균수는 적송수피추출물 80 ppm 첨가구가 대조구 및 항생제 첨가구에 비해서 높았으나 Escherichia coli, Salmonella 균수는 감소하였으며 처리구 간 통계적인 유의차가 인정되었다. 본 연구결과는 브로일러 사료 내 항생제 대체제로서 국산 적송수피추출물 80 ppm을 첨가, 급여해주면 브로일러의 성장능력, 도체특성, 면역능력 향상 및 유익한 맹장미생물의 활성을 높여줄 수 있음을 시사해준다.

This study was conducted to evaluate the effects of dietary Korean red pine bark extract as an antibiotic replacement on cecum microflora, immune functions, blood lipids, carcass characteristics, and growth performance in broiler chickens. There were four treatment groups: a control group, an antibiotic group, and two treatment groups receiving 65 and 80 ppm Korean red pine bark extract. Growth performance, carcass weight, dressing percentage, and breast and thigh muscle weight of the broiler chickens fed 80 ppm of red pine bark extract was higher than the broilers of the control group and the antibiotic group. Abdominal fat was lower in both groups of broiler chickens fed red pine bark extract, but the growth of immune organs such as the thymus, spleen, and bursa of Fabricius was significantly higher in the group that received a diet supplemented with 80 ppm red pine bark extract than in the control group and antibiotic group. Concentrations of triacylglyceride and total cholesterol were significantly lower in both groups that received red pine bark extract when compared to the control group and the antibiotic group. The concentration of serum IgG was significantly higher in the 80 ppm red pine bark extract group than in the control group and the antibiotic group. The numbers of Bifidobacterium and Lactobacillus in the cecum digesta were significantly increased, while the numbers of Escherichia coli and Salmonella were significantly reduced in the group that received 80 ppm red pine bark extract when compared to the control group and the antibiotic group. These results suggest that the a diet with 80 ppm Korean red pine bark extract as a replacement for antibiotics improve the cecum microflora, immune functions, blood lipids, carcass characteristics and growth performance of broiler chickens.

키워드

참고문헌

  1. Ahn, J., I. U. Grun, and A. Mustapha. 2007. Effects of plant extracts on microbial growth, color change, and lipid oxidation in cooked beef. Food Microbiol. 24, 7-14. https://doi.org/10.1016/j.fm.2006.04.006
  2. Cheshier, J. E., S. Ardestani-Kaboudanian, B. Liang., M. Araghiniknam, S. Chung, L. Lane, A. Castro, and R. R. Watson. 1996. Immunomodulation by pycnogenol in retrovirus-infected or ethanol fed mice. Life Sci. 58, 87-96.
  3. Choi, J. H., M. K. Choi, O. T. Han., S. J. Han, S. J. Chung, C. K. Shim, and D. D. Kim. 2007. Evaluation of skin absorptio of catechin from topical formulations containing Korean pine bark extract ($pinexol^{\circledR}$). J. Kor. Pharm. Sci. 37, 359-364. https://doi.org/10.4333/KPS.2007.37.6.359
  4. Close, B., K. Banister, V. Baumans, E. M. Bernoth, N. Bromage, J. Bunyan, W. Erhardt, P. Flecknell, N. Gregory, H. Hackbarth, D. Morton, and C. Warwick. 1997. Recommendations for euthanasia of experimental animals, Part 2. Laboratory Animals 31, 1-32. https://doi.org/10.1258/002367797780600297
  5. Cowan, M. M. 1999. Plant products as antimicrobial agents. Clin. Microbiol. Rev. 12, 564-582.
  6. Devaraj, S., S. Vega-Lopez, N. Kaul, F. Schönlau, P. Rohdewald, and I. Jialal. 2002. Supplementation with a pine bark extract rich in polyphenols increases plasma antioxidant capacity and alters the plasma lipoprotein profile. Lipids 37, 931-934. https://doi.org/10.1007/s11745-006-0982-3
  7. Dibner, J. J. and J. D. Richards. 2005. Antibiotic growth promoters in agriculture: History and mode of action. Poult. Sci. 84, 634-643. https://doi.org/10.1093/ps/84.4.634
  8. Dickens, J. A., M. E. Berrang, and N. A. Cox. 2000. Efficacy of an herbal extract on the microbiological quality of broiler carcass during a simulated chill. Poult. Sci. 79, 1200-1203. https://doi.org/10.1093/ps/79.8.1200
  9. Gibson, G. R. and X. Wang. 1994. Bifidogenic properties of different types of fructooligosaccharides. Food Microbiol. 11, 491-498. https://doi.org/10.1006/fmic.1994.1055
  10. Grimm. T., Z. Chovanova, J. Muchova, K. Sumegova, A. Liptakova, Z. Durackova, and P. Hogger. 2006. Inhibition of $NF-_{\kappa}B$ activation and MMP-9 secretion by plasma of human volunteers after ingestion of maritime pine bark extract (Pycnogenol). J. Inflammation 3, 1-8. https://doi.org/10.1186/1476-9255-3-1
  11. Hasegawa, N. 2000. Inhibition of lipogenesis by pycnogenol. Phytotherapy Res. 14, 472-473. https://doi.org/10.1002/1099-1573(200009)14:6<472::AID-PTR649>3.0.CO;2-S
  12. Hasegawa, N. 1999. Stimulation of lipolysis by pycnogenol. Phytotherapy Res. 13, 19-620.
  13. Hong, B. J., J. S. Oh, B. W. Kim, and B. S. Park. 2008. Effect of feeding dietary pitamin as a organic livestock feed additives in laying hens. Kor. J. Organic Agric. 16, 205-218.
  14. Ikeguchi, M., T. Masahito, T. Atsushi, and T. Kinya. 2006. Effects of pine bark extract on lipid metabolism in rats. J. Japan Soc. Nutr. Food Sci. 59, 89-95. https://doi.org/10.4327/jsnfs.59.89
  15. Kim, B. W., J. S. Oh, O. T. Han, S. O. Park, and B. S. Park. 2009. Effect of pitamin as an antibiotics replacement for or ganic livestock feed additives in broiler chickens. Kor. J. Organic Agric. 17, 111-125.
  16. Lee, O. H., K. Y. Kim, M. K. Jang, K. H. Yu, S. G. Kim, M. H. Kim, and S. H. Lee. 2008. Evaluation of proanthocyanidin contents in total polyphenolic compounds of pine (pinus densiflora) needle extracts and their antioxidative activities. J. Life Sci. 18, 213-219. https://doi.org/10.5352/JLS.2008.18.2.213
  17. Mochizuki, M. and N. Hasegawa. 2004. Pycnogenol stimulates lipolysis in 3t3-L1 cells via stimulation of ${\beta}-receptor$ mediated activity. Phytotherapy Res. 18, 1029-1030. https://doi.org/10.1002/ptr.1612
  18. National Research Council. 1994. Nutrient Requirements of Poultry. 9th rev. eds., National Academy Press, Washington, DC.
  19. Orrhage, K. and C. E. Nord. 2000. Bifidobacteria and lactobacilli in human health. Drugs Exp. Clin. Res. 26, 95-111.
  20. Ozlem, Y. C., M. Ganzera, I. Akgun, C. Sevimli, K. Korkmaz, and E. Bedir. 2009. Determination of polyphenolic constituents and biological activities of bark extracts from different Pinus species. J. Sci. Food Agric. 89, 1339-1345. https://doi.org/10.1002/jsfa.3591
  21. Rohdewald, P. A. 2002. A review of the French maritime pine bark extract (pycnogenol), an herbal medication with a diverse clinical pharmacology. Int. J. Clin. Pharmacol. Ther. 40, 158-168. https://doi.org/10.5414/CPP40158
  22. SAS. 2004. SAS/STAT User's Guide: Statistics. SAS Inst. Inc., Cary, NC.
  23. Scot PIL training manual. 1994. Glasgow Univ. UK.
  24. Silliman, K., J. Parry., L. L. Kirk, and R. L. Prior. 2003. Pycnogenol does not impact the antioxidant or vitamin C status of healthy young adults. J. Am. Diet Assoc. 103, 67-72.
  25. Torras, M. A., C. A. Faura, F. Schonlau, and P. Rohdewald. 2005. Antimicrobial activity of pycnogenol. Phytotherapy Res. 19, 647-648. https://doi.org/10.1002/ptr.1662
  26. Wang, Y. W., C. J. Field, and J. S. Sim. 2000. Dietary polyunsaturated fatty acids alter lymphocyte subset proportion and proliferation, serum immunoglobulin G concentration, and immune tissue development in chicks. Poult. Sci. 79, 1741-1748. https://doi.org/10.1093/ps/79.12.1741

피인용 문헌

  1. Antimicrobial and antiviral activity of Saururus chinensis extract by n-Hexane vol.36, pp.2, 2013, https://doi.org/10.7853/kjvs.2013.36.2.87