Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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The Extracts from Liriope platyphylla Significantly Stimulated Insulin Secretion in the HIT-T15 Pancreatic β-Cell Line

HIT-T15 췌장세포의 인슐린분비 촉진을 유도하는 맥문동(Liriope platyphylla) 추출물의 효능 및 독성분석

  • Kim, Ji-Ha (Department of Biomaterials Science, College of Natural Resources and Life Science, Pusan National University) ;
  • Kim, Ji-Eun (Department of Biomaterials Science, College of Natural Resources and Life Science, Pusan National University) ;
  • Lee, Yoen-Kyung (Department of Biomaterials Science, College of Natural Resources and Life Science, Pusan National University) ;
  • Nam, So-Hee (Department of Biomaterials Science, College of Natural Resources and Life Science, Pusan National University) ;
  • Her, Youn-Kyung (Department of Biomaterials Science, College of Natural Resources and Life Science, Pusan National University) ;
  • Jee, Seoung-Wan (Department of Laboratory Animal Resources, National Institute of Toxicological Research, Korean FDA) ;
  • Kim, Sun-Guen (Department of Horticultural Bioscience, College of Natural Resources and Life Science, Pusan National University) ;
  • Park, Da-Jung (Department of Horticultural Bioscience, College of Natural Resources and Life Science, Pusan National University) ;
  • Choi, Young-Whan (Department of Horticultural Bioscience, College of Natural Resources and Life Science, Pusan National University) ;
  • Hwang, Dae-Youn (Department of Biomaterials Science, College of Natural Resources and Life Science, Pusan National University)
  • 김지하 (부산대학교 생명자원과학대학 바이오소재과학과) ;
  • 김지은 (부산대학교 생명자원과학대학 바이오소재과학과) ;
  • 이연경 (부산대학교 생명자원과학대학 바이오소재과학과) ;
  • 남소희 (부산대학교 생명자원과학대학 바이오소재과학과) ;
  • 허윤경 (부산대학교 생명자원과학대학 바이오소재과학과) ;
  • 지승완 (식품의약품안전연구원 실험동물자원과) ;
  • 김선건 (부산대학교 생명자원과학대학 원예생명과학과) ;
  • 박다정 (부산대학교 생명자원과학대학 원예생명과학과) ;
  • 최영환 (부산대학교 생명자원과학대학 원예생명과학과) ;
  • 황대연 (부산대학교 생명자원과학대학 바이오소재과학과)
  • Received : 2010.03.02
  • Accepted : 2010.04.15
  • Published : 2010.07.30
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Abstract

Liriope platyphylla has traditionally been used in Korea and China as a therapeutic drug for the treatment of coughing, sputum, neurodegenerative disorders, obesity, and diabetes. In an effort to assess the functions of a novel extract from Liriope platyphylla in diabetes therapy, the insulin secretion abilities of 10 extracts were screened via measurements of insulin concentration in the culture supernatant using an Insulin ELISA kit. The results of this assay showed the highest levels of insulin in the LP9M80-H treated group, followed by the LP-H, LP-M, LP-E and LP9M80-C treated groups, whereas other extracts did not induce insulin secretion in the HIT-T15 cells. However, the extracts capable of stimulating insulin secretion simultaneously evidenced high apoptotic activity as compared with other extracts. Therefore, one of these extracts, LP9M80-H, was initially selected as the optimal candidate for a therapeutic drug and its optimal concentration was determined. The results of the ELISA and MTT assay demonstrated that a concentration of approximately 100-125 ug/ml of LP9M80-H was optimal with regards to cell viability and insulin secretion in the HIT-T15 cells. These results suggest that LP9M80-H could be considered as an excellent candidate for a diabetes-therapeutic drug that could induce insulin secretion in pancreatic $\beta$-cells.

맥문동(Liriope platyphylla)은 한국과 중국에서 전통적으로 당뇨, 비만, 뇌신경질환, 천식 등의 치료를 위해 사용 해온 치료제이다. 최근에 이들 맥문동으로부터 새로운 치료제를 개발하려는 노력이 활발히 진행 중이지만 아직도 유력한 치료후보제는 확보되지 않았다. 따라서 본 연구에서는 맥문동의 새로운 추출물을 이용하여 당뇨치료 제로서의 가능성을 평가하기 위하여 새로운 방법으로 10가지 후보물질을 추출하고 이들의 독성과 효능을 평가하였다. 그 결과 10가지 추출물 중에서 LP9M80-H가 인슐린 분비를 가장 많이 촉진하였고 다음으로는 LP-H, LP-M, LP-E과 LP9M80-C 등의 순서로 촉진을 하였으나 나머지는 인슐린 분비를 촉지하지 못하였다. 그러나 이들 물질은 인슐린 분비를 촉진하는 농도에서 강한 세포 독성을 나타내었다. 따라서 이들 물질 중에서 가장 효능이 좋은 LP9M80-H의 치료용 최적농도를 설정하였으며, 대략 100-25 ug/ml가 최적농도로 결정되었다. 이러한 결과는 맥문동 추출물 중에서 LP9M80-H가 췌장 $\beta$-세포의 인슐린 분비능을 유도하는 새로운 당뇨치료 후보물질로서 향후에 사용될 가능성을 시사하고 있다.

Keywords

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