Journal of Industrial Technology (산업기술연구)
- Volume 30 Issue B
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- Pages.69-74
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- 2010
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- 1229-9588(pISSN)
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- 1598-1371(eISSN)
Enhancement of chitinolytic activity of by co-expression of endochitinase and chitobiosidase genes
Endochitinase와 Chitobiosidase 유전자의 동시발현에 의한 키틴분해 활성의 증가
Abstract
Chitinolytic activity was enhanced by coexpression of endo-chitinase gene (chiA) and chitobiosidase gene (chiB) from Serratia marcescens KFRI314 using constitutive expression vector, pHCEIA, in E. coli. Coexpression vector was constructed by inserting ribosome binding site (RBS) into junction between two chitinase genes. SDS-PAGE analyses showed that two chitinase were constitutively expressed while E. coli clones expressing two chitinases simultaneously increased halo size on colloidal chitin plate. Furthermore, the chitinolytic activities were much enhanced in coexpressed clones when degradation patterns of substrate analogues such as 4-MU-(NAG),