한국어병학회지 (Journal of fish pathology)

  • 제23권3호
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  • Pages.429-440
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  • 2010
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  • 1226-0819(pISSN)
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  • 2233-5412(eISSN)
한국어병학회 (The Korean Society of Fish Pathology)
권호 표지

Expressed sequence tags analysis of immune-relevant genes in rock bream Oplegnathus fasciatus gill stimulated with LPS

  • Lee, Jeong-Ho (Genetics and Breeding Research Center, National Fisheries Research and Development Institute) ;
  • Kim, Ju-Won (Department of Marine Biology & Aquaculture, Institute of Marine Industry, College of Marine Science, Gyeongsang National University) ;
  • Baeck, Gun-Wook (Department of Marine Biology & Aquaculture, Institute of Marine Industry, College of Marine Science, Gyeongsang National University) ;
  • Park, Chan-Il (Department of Marine Biology & Aquaculture, Institute of Marine Industry, College of Marine Science, Gyeongsang National University)
  • 투고 : 2010.09.03
  • 심사 : 2010.11.16
  • 발행 : 2010.12.31
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초록

We constructed a rock bream (Oplegnathus fasciatus) gill cDNA library and a total of 1450 expressed sequence tag (EST) clones were generated. Gene annotation procedures and homology searches of the sequenced ESTs were locally done by BLASTX for amino acid similarity comparisons. Of the 1450 EST clones, 1022 EST clones showed significant homology to previously described genes while 428 ESTs were unidentified, and 259 clones were hypothetical, or unnamed proteins. Encoding 313 different sequences were identified as putative bio-defense genes or genes associated with immune response.

키워드

참고문헌

  1. Azam, A., J. Paul, D. Sehgal, J. Prasad. S. Bhattacharya and A. Bhattacharya. Identification of novel genes from Entamoeba histolytica by expressed sequence tag analysis. Gene, 181: 113-116. 1996. https://doi.org/10.1016/S0378-1119(96)00484-2
  2. Cho, Y.S., B.N. Choi, K.H. Kim, S.K. Kim, D.S. Kim, I.C. Bang and Y.K. Nam. Differential expression of Cu/Zn superoxide dismutase mRNA during exposures to heavy metals in rockbream (Oplegnathus fasciatus). Aquaculture, 253: 667-679. 2006. https://doi.org/10.1016/j.aquaculture.2005.05.047
  3. Clark, M.S., Y.J. Edwards, D. Peterson, S.W. Clifton, A.J. Thompson, M. Sasaki, Y. Suzuki, K. Kikuchi, S. Watabe, K. Kawakami, S. Sugano, G. Elgar and S.L. Johnson. Fugu ESTs: new resources for transcription analysis and genome annotation. Genome Res., 13:2747-2753. 2003. https://doi.org/10.1101/gr.1691503
  4. Cole, A.M., R.O. Darouiche, D. Legarda, N. Connell, G. Diamond. Characterization of a fish antimicrobial peptide: gene expression, subcellular localization, and spectrum of activity. Antimicrob. Agents Chemother., 44: 2039-2045. 2000. https://doi.org/10.1128/AAC.44.8.2039-2045.2000
  5. Franco, G.R., M.D. Adams, S.M. Bento, A.J.G. Simpson, J.C. Venter and S.D.J. Pena. Identification of new Schistosoma mansonigenes by the EST strategy using a directional cDNA library. Gene, 152: 141-147. 1995. https://doi.org/10.1016/0378-1119(94)00747-G
  6. Gish, W and J.S. David. Identification of protein coding regions by database similarity search. Nature Genetics, 3: 266-272. 1993. https://doi.org/10.1038/ng0393-266
  7. Hjelmeland, K., M. Christie and J. Raa. Skin mucus protease from rainbow trout, Salmo gairdneri Richardson, and its biological significance. J. Fish Biol., 23: 13-22. 1983. https://doi.org/10.1111/j.1095-8649.1983.tb02878.x
  8. Honda, S., M. Kashiwagi, K. Miyamoto, Y. Takei and S. Hirose. Multiplicity, structures, and endocrine and exocrine natures of eel fucose-binding lectins. J. Biol. Chem., 275: 33151-33157. 2000. https://doi.org/10.1074/jbc.M002337200
  9. Jung S.J. and M.J. Oh. Iridovirus-like infection associated with high mortalities of striped beakperch, Oplegnathus fasciatus (Temminck et Schlegel) in southern coastal areas of the Korean peninsula. J. Fish Dis., 23: 223-226. 2000. https://doi.org/10.1046/j.1365-2761.2000.00212.x
  10. Klingel, K., J.J. Schnorr, M. Sauter, G. Szalay and R. Kandolf. ${\beta}2$-microglobulin associated regulation of interferon-gamma and virus-specific immunoglobulin G confer resistance against the development of chronic coxsackievirus myocarditis. Am. J. Pathol. 162: 1709-1720. 2003. https://doi.org/10.1016/S0002-9440(10)64305-2
  11. Lee, C.K., R. Weindruch and T.A. Prolla. Gene-expression profile of the aging skin in mice. Nat. Genet., 25: 294-297. 2000. https://doi.org/10.1038/77046
  12. Maruyama, K. and S. Sugano. Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides. Gene, 138: 171-174. 1994. https://doi.org/10.1016/0378-1119(94)90802-8
  13. Michaelsson, J., A. Achour, A. Rolle and K. Karre. MHC class I recognition by NK receptors in the Ly49 family is strongly influenced by the beta 2-microglobulin subunit. J. Immunol. 166: 7327-7334. 2001.
  14. Mistry, A.C., S. Honda and S. Hirose. Structure, properties and enhanced expression of galactose-binding C-type lectins in mucous cells of gills from freshwater Japanese eels (Anguilla japonica). Biochemistry, 360: 107-115. 2001. https://doi.org/10.1042/0264-6021:3600107
  15. Mori, M., Y. Terui, M. Ikeda, H. Tomizuka, M. Uwai and T Kasahara. Beta(2)-microglobulin identified as an apoptosis-inducing factor and its characterization. Blood, 94: 2744-2753. 1999.
  16. Rise, M.L., K.R. von Schalburg, G.D. Brown, M.A. Mawer, R.H. Devlin, N. Kuipers, M. Busby, M. Beetz-Sargent, R. Alberto, A.R. Gibbs, P. Hunt, R. Shukin, J.A. Zeznik, C. Nelson, S.R. Jones, D.E. Smailus, S.J. Jones, J.E. Schein, M.A. Marra, Y.S. Butterfield, J.M. Stott, S.H. Ng, W.S. Davidson and B.F. Koop. Development and application of a salmonid EST database and cDNA microarray: data mining and interspecific hybridization characteristics. Genome Res., 14: 478-490. 2004. https://doi.org/10.1101/gr.1687304
  17. Russell, S. and J.S. Lumsden. Function and heterogeneity of fish lectins. Vet. Immunol. Immunopathol., 108: 111-120. 2005. https://doi.org/10.1016/j.vetimm.2005.08.004
  18. Shephard, K.L. Functions for fish mucus. Rev. Fish Biol. Fisher., 4: 401-409. 1994. https://doi.org/10.1007/BF00042888
  19. Sohn, S.G., D.L. Choi, J.W. Do, G.Y. Hwang and J.W. Park. Mass mortalities of cultured striped beakperch, Oplegnathus fasciatus by iridoviral infection. J. Fish Pathol., 13: 121-127. 2000.
  20. Tania, C., L.Z. Saraiva, E.N. Grund, D.B.S. Komegae, D.R. Anderson, C. Katia, M. Noemia C.L. Orii and L.F. Monica. Nattectin a fish C-type lectin licenses macrophages to differentiate into cells exhibiting typical dendritic cells function. Cytokine, 48: 107. 2009.
  21. Ton, C., D.M. Hwang, A.A. Dempsey, H.C. Tang, J. Yoon, M. Lim, J.D. Mably, M.C. Fishman and C.C. Liew. Identification, characterization, and mapping of expressed sequence tags from an embryonic zebrafish heart cDNA library. Genome Res., 10: 1915-1927. 2000. https://doi.org/10.1101/gr.10.12.1915
  22. Zelensky, A.N. and J.E. Gready. C-type lectin-like domains in Fugu rubripes. BMC Genomics, 5: 51. 2004. https://doi.org/10.1186/1471-2164-5-51
  23. Zeng, S. and Z. Gong. Expressed sequence tag analysis of expression profiles of zebrafish testis and ovary. Gene, 10: 45-53. 2002.