Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
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Identification of Microorganisms Isolated from Wound Regions of Chickens

계육의 창상부위에서 분리된 미생물의 동정

  • Kim, Hyun-Jin (Department of Food Science & Technology, Chungnam National University) ;
  • Chun, Ho-Hyun (Department of Food Science & Technology, Chungnam National University) ;
  • Kim, Ju-Yeon (Department of Food Science & Technology, Chungnam National University) ;
  • Jang, Sung-Ae (Department of Food Science & Technology, Chungnam National University) ;
  • Lee, Bong-Duk (Department of Animal Science & Biotechnology, Chungnam National University) ;
  • Chae, Hyun-Seok (Poultry Science Division, National Institute of Animal Science) ;
  • Song, Kyung-Bin (Department of Food Science & Technology, Chungnam National University)
  • Received : 2009.11.30
  • Accepted : 2010.03.19
  • Published : 2010.04.30
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Abstract

We identified microorganisms causing skin disease in slaughtered chickens. Ten microbial strains were isolated from skin wound regions on the back and legs of chickens. Fatty acid composition analysis of the cell membranes of isolated bacteria identified five isolates of Shigella sonnei, Proteus mirabilis (2), and Escherichia coli (2). In addition, 16S rRNA sequencing indicated that S. sonnei (99%), P. mirabilis (99%), and E. coli (99%) were the strains responsible for skin wounds in chickens. Therefore, these three species may be the major pathogenic bacteria causing skin wounds on the back and legs of chickens.

본 연구에서는 계육 창상부위의 원인이 되는 세균을 동정하였다. 계육의 등과 다리 창상 부위로부터 무작위 선별법을 통해 총 10주의 균주를 분리하였다. 순수 분리 배양된 균주의 세포막 지방산 조성 분석 결과, 5 균주가 높은 유효성을 지녔으며, Shigella sonnei, Proteus mirabilis, Escherichia coli로 동정되었다. 또한 16S rRNA sequence를 실시한 결과, 동정된 균주는 Shigella sonnei(99%), Proteus mirabilis(99%) 그리고 Escherichia coli(99%)와 높은 염기서열 상동성을 나타냈다. 따라서 본 연구에서 조사된 계육의 등과 다리 부위 주요 창상 원인균은 Shigella sonnei, Proteus mirabilis, Escherichia coli인 것으로 밝혀졌다.

Keywords

References

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