Reproductive and Developmental Biology

The Korean Society of Animal Reproduction (한국동물번식학회)
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Study on the In Vitro Maturation and Sperm Penetration Rates of Canine Oocytes

  • Park, Ji-Hoon (College of Veterinary Medicine, Chungnam National University) ;
  • Seok, Ho-Bong (College of Bio-resources Science, Dankook University) ;
  • Kim, Sang-Keun (College of Veterinary Medicine, Chungnam National University)
  • Received : 2010.03.10
  • Accepted : 2010.03.22
  • Published : 2010.03.31
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Abstract

The purpose of this study was to investigate the effects of the collection time, co-culture and sperm penetration of canine oocytes on in vitro maturation and fertilization. The oocytes were cultured in TCM-199 media containing hormonal supplements (10% FCS, 10 IU/ml HCG, 10 IU/ml PMSG) at 5% $CO_2$, 95% air, $38^{\circ}C$. The in vitro maturation rate to MII stage of in vitro oocytes recovered from ovaries that collected at follicular, luteal and inactive phases of the reproductive phase for 44~72 hrs were 19.2%, 12.2%, and 6.0%, respectively. Follicular phases oocytes had a significantly higher in vitro maturation rate than oocytes collected at luteal and anestrus stage (p<0.05). The in vitro maturation rates to the MII stage of canine oocytes after 48 hrs of culture with glutathione, pyruvate, or glutathione + pyruvate were 12.5%, 10.7%, and 17.5%, respectively. This was higher than that in both alone or the combination of the two compared to the control group (19.0%). The sperm penetration rates of in vitro matured oocytes by fresh and frozen semen were 29/80 (36.3%) and 18/80 (22.5%), respectively. Although there are limited reports about canine oocytes co-culture and in vitro fertilization, our results on in vitro maturation is comparable to the results from other researches.

Keywords

References

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