Reproductive and Developmental Biology

The Korean Society of Animal Reproduction (한국동물번식학회)
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Differentiation of Mesenchymal Stem Cell-like Cell from Feeder Free Cultured Human Embryonic Stem Cells using Direct Induction System

Feeder-free에서 배양된 인간배아줄기세포의 직접분화유도 방법을 이용한 간엽줄기세포로의 분화

  • Lee, Min-Ji (Graduate School of Life Science, CHA Stem Cell Institute, CHA University, College of Medicine) ;
  • Lee, Jae-Ho (CHA Biotech & Diostech Co. Ltd.) ;
  • Kim, Ju-Mi (CHA Biotech & Diostech Co. Ltd.) ;
  • Shin, Jeong-Min (CHA Biotech & Diostech Co. Ltd.) ;
  • Park, Soon-Jung (Graduate School of Life Science, CHA Stem Cell Institute, CHA University, College of Medicine) ;
  • Chung, Sun-Hwa (Graduate School of Life Science, CHA Stem Cell Institute, CHA University, College of Medicine) ;
  • Lee, Kyung-Il (Graduate School of Life Science, CHA Stem Cell Institute, CHA University, College of Medicine) ;
  • Chae, Jung-Il (Graduate School of Life Science, CHA Stem Cell Institute, CHA University, College of Medicine) ;
  • Chung, Hyung-Min (Graduate School of Life Science, CHA Stem Cell Institute, CHA University, College of Medicine)
  • 이민지 (차의과학대학 의생명과학대학원 줄기세포 연구실) ;
  • 이재호 (차바이오앤디오스텍) ;
  • 김주미 (차바이오앤디오스텍) ;
  • 신정민 (차바이오앤디오스텍) ;
  • 박순정 (차의과학대학 의생명과학대학원 줄기세포 연구실) ;
  • 정선화 (차의과학대학 의생명과학대학원 줄기세포 연구실) ;
  • 이경일 (차의과학대학 의생명과학대학원 줄기세포 연구실) ;
  • 채정일 (차의과학대학 의생명과학대학원 줄기세포 연구실) ;
  • 정형민 (차의과학대학 의생명과학대학원 줄기세포 연구실)
  • Received : 2009.12.04
  • Accepted : 2010.03.25
  • Published : 2010.03.31
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Abstract

Mesenchymal stem cells (MSCs) have the multipotent capacity and this potential can be applied for obtaining valuable cell types which can use for cell therapy on various regenerative diseases. However, insufficient availability of cellular source is the major problem in cell therapy field using adult stem cell sources. Recently, human embryonic stem cells (hESCs) have been highlighted to overcome a limitation of adult cellular sources because they retain unlimited proliferation capacity and pluripotency. To use of hESCs in cell therapy, above all, animal pathogen free culture system and purification of a specific target cell population to avoid teratoma formation are required. In this study, we describe the differentiation of a mesenchymal stem cell-like cells population from feeder-free cultured hESCs(hESC-MSCs) using direct induction system. hESC-MSCs revealed characteristics similar to MSCs derived from bone marrow, and undifferentiated cell markers were extremely low in hESC-MSCs in RT-PCR, immunostaining and FACS analyses. Thus, this study proffer a basis of effective generation of specialized human mesenchymal stem cell types which can use for further clinical applications, from xenofree cultured hESCs using direct induction system.

Keywords

Acknowledgement

Supported by : 교육과학부 21세기 프론티어 사업단 stem cell research center

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