Journal of the Korean Wood Science and Technology

The Korean Society of Wood Science & Technology (한국목재공학회)
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Selection of White Rot Fungi for Biodegradation of Polychlorinated Biphenyl, and Analysis of Its Biodegradation Rate

폴리염화비페닐류의 생분해 우수 백색부후균 선발 및 분해율 분석

  • Hong, Chang-Young (Department of Forest Sciences, College of Agriculture & Life Sciences, Seoul National University) ;
  • Gwak, Ki-Seob (Department of Forest Sciences, College of Agriculture & Life Sciences, Seoul National University) ;
  • Lee, Su-Yeon (Department of Forest Sciences, College of Agriculture & Life Sciences, Seoul National University) ;
  • Kim, Seon-Hong (Department of Forest Sciences, College of Agriculture & Life Sciences, Seoul National University) ;
  • Choi, In-Gyu (Department of Forest Sciences, College of Agriculture & Life Sciences, Seoul National University)
  • 홍창영 (서울대학교 농업생명과학대학 산림과학부) ;
  • 곽기섭 (서울대학교 농업생명과학대학 산림과학부) ;
  • 이수연 (서울대학교 농업생명과학대학 산림과학부) ;
  • 김선홍 (서울대학교 농업생명과학대학 산림과학부) ;
  • 최인규 (서울대학교 농업생명과학대학 산림과학부)
  • Received : 2010.07.07
  • Accepted : 2010.08.21
  • Published : 2010.11.25
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Abstract

In this study, the possibility of biodegradation of polychlorinated biphenyls (PCBs) by various white rot fungi was evaluated, and outstanding white rot fungi for the degradation of PCBs were selected. Seven white rot fungi were used to degrade Aroclor 1254 and 1260, which are widely considered to be toxic and difficult to degrade. And the degradation rates of Aroclors by selected white rot fungi were performed by GC analysis. Through the resistance test of white rot fungi on different concentrations of PCBs, the inhibition of mycelial growth of Cystidodontia isubellina was much less than that of others, and this fungus grew faster than others, relatively. Based on this result, it was considered that C. isubellina was selected as degrading fungus for Aroclors. As a result of biodegradation rate of Aroclors by Cystidodontia isubellina, the degradation rate of Arolor 1254 was reached to 57.57% in 13 days, which showed very high degradation rate. Also the degradation rate of Aroclor 1260 by C. isubellina had a tendency of increasing along with increasing incubation day. Maximal degradation rate of Aroclor 1260 was 49.43% at 13 days. Based on this results, it indicated that in comparison with a previous study, high degradation rate was obtained by C. isubellina.

본 연구에서는 다양한 목재부후균의 polychlorinated biphenyls (PCBs) 생분해 가능성을 구명하고자 7종의 백색부후균을 이용하여 PCB 혼합물 중 가장 분해가 어렵다고 알려진 Aroclor 1254와 1260을 대상으로 분해력이 우수한 목재부후균을 선발하였다. 그리고 선발된 목재부후균을 이용하여 GC 분석을 통해 Aroclor 1254, 1260의 분해율을 조사하였다. 고체배지 저항성 테스트를 통해 우수분해 균주로 선발된 Cystidodontia isubellina 는 다른 균주에 비해 균사생장량의 억제가 가장 적었으며 상대적으로 생장속도도 빠른 특징을 나타냈다. 선발된 C. isubellina에 의한 Aroclor 1254와 1260의 분해율 분석 결과, Aroclor 1254배양 13일째 57.57%로 가장 높은 분해율을 나타냈다. Aroclor 1260 역시 배양일수가 증가함에 따라 분해율 역시 증가하는 경향을 보였으며, 배양 13일째 49.43%로, 두 혼합물 모두 기존의 다른 연구에서보다 높은 분해율을 나타냈다.

Keywords

References

  1. Abramowicz, D. 1990. Aerobic and anaerobic bio-degradation of PCBs: a review. Critical Reviews in Biotechnology 10(3): 241-251. https://doi.org/10.3109/07388559009038210
  2. Beaudette, L., S. Davies, P. Fedorak, O. Ward, and M. Pickard. 1998. Comparison of gas chromatography and mineralization experiments for measuring loss of selected polychlorinated biphenyl congeners in cultures of white rot fungi. Applied and Environmental Microbiology 64(6): 2020-2025.
  3. Bedard, D., R. Wagner, M. Brennan, M. Haberl, and J. Brown Jr. 1987. Extensive degradation of Aroclors and environmentally transformed polychlorinated biphenyls by Alcezligznes eutrophus H850. Applied and Environmental Microbiology 53(5): 1094-1102.
  4. Borja, J., D. Taleon, J. Auresenia, and S. Gallardo, 2005. Polychlorinated biphenyls and their biodegradation. Process Biochemistry 40(6): 1999-2013. https://doi.org/10.1016/j.procbio.2004.08.006
  5. Boyle, A., C. Silvin, J. Hassett, J. Nakas, and S. Tanenbaum. 1992. Bacterial PCB biodegradation. Biodegradation 3(2): 285-298. https://doi.org/10.1007/BF00129089
  6. Dietrich, D., W. Hickey, and R. Lamar. 1995. Degradation of 4, 4'-dichlorobiphenyl, 3, 3' 4, 4'-tetrachlorobiphenyl, and 2, 2' 4, 4' 5, 5'-hexa-chlorobiphenyl by the white rot fungus Pha-nerochaete chrysosporium. Applied and Environmental Microbiology 61(11): 3904-3909.
  7. Dmochewitz, S. and K. Ballschmiter. 1988. Microbial transformation of technical mixtures of polychlorinated biphenyls (PCB) by the fungus. Chemosphere 17(1): 111-121. https://doi.org/10.1016/0045-6535(88)90049-5
  8. Eaton, D. 1985. Mineralization of polychlorinated biphenyls by Pbanerochaete chrysosporium:a ligninolytic fungus. Enzyme and Microbial Technology 7(5): 194-196. https://doi.org/10.1016/S0141-0229(85)80001-6
  9. Huynh, V. and Crawford, R. 1985. Novel extracellular enzymes (ligninases) of Phanerochaete chrysosporium. FEMS Microbiology Letters 28(1): 119-123. https://doi.org/10.1111/j.1574-6968.1985.tb00776.x
  10. Krcmar, P., A. Kubatova, J. Votruba, P. Erbanova, Novotn, and V. Sasek. 1999. Degradation of poly-chlorinaled biphenyls by extracellular enzymes of Phanerochaete chrysosporium produced in a perforated plate bioreactor. World Journal of Microbiology and Biotechnology 15(2): 269-276. https://doi.org/10.1023/A:1008994912875
  11. Quensen III, J., S. Boyd, and J. Tiedje. 1990. Dechlorination of four commercial polychlorinated biphenyl mixtures (Aroclors) by anaerobic microorganisms from sediments. Applied and Environmental Microbiology 56(8): 2360-2369.
  12. Takagi, S., C. Shirota, K. Sakaguchi, J. Suzuki, T. Sue, H. Nagasaka, S. Hisamatsu, and S. Sonoki. 2007. Exoenzymes of Trametes versicolor can metabolize coplanar PCB congeners and hydroxy PCB. Chemosphere 67(9): 54-57. https://doi.org/10.1016/j.chemosphere.2006.05.090
  13. Thomas, D., K. Carswell, and G. Georgiou. 1992. Mineralization of biphenyl and PCBs by the white rot fungus Phanerochaete cbrysosporium. Biotechnology and bioengineering 40(11): 1395-1402. https://doi.org/10.1002/bit.260401114
  14. Tien, M. 1987. Properties of ligninase from Phanerochaete chrysosporium and their possible applications. Critical Reviews in Microbiology 15(2): 141-168. https://doi.org/10.3109/10408418709104456
  15. Yadav, J, J. Quensen III, J. Tiedje, and C. Redely. 1995. Degradation of polychlorinated biphenyl mixtures (Aroclors 1242 1254, and 1260) by the white rot fungus Phanerochatet chrysosporium as evidenced by congener-specific analysis. Applied and Environmental Microbiology 61(7): 2560-2565.
  16. Zachar, P., C. Novotn, Z. Voznakova, M. Matuchac, E. Tesa rova, D. Skora, A. Kubatova, M. Popl. and V. Sasek. 1996. Physical factors negatively affecting evaluation of long-term biodegradation experiments of polychlorinated biphenyls. Chemosphere 33(12): 2411-2421. https://doi.org/10.1016/S0045-6535(96)00338-4

Cited by

  1. Ceriporia sp. ZLY-2010 in Biodegradation of Polychlorinated Biphenyls : Extracellular Enzymes Production and Effects of Cytochrome P450 Monooxygenase vol.39, pp.6, 2011, https://doi.org/10.5658/WOOD.2011.39.6.469
  2. Biodegradation of PCB congeners by white rot fungus,Ceriporiasp. ZLY-2010, and analysis of metabolites vol.47, pp.12, 2012, https://doi.org/10.1080/03601234.2012.676432