Journal of the East Asian Society of Dietary Life (동아시아식생활학회지)

The East Asian Society of Dietary Life (동아시아식생활학회)
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The Effect of Blueberry Extract on Gene Expressions Related to Apoptosis in Human Breast Cancer MCF7 Cells

블루베리가 인체 유방암세포 MCF7에서 세포 사멸 관련 유전자 발현에 미치는 영향

  • Lee, Se-Na (Dept. of Food & Nutrition, DukSung Women's University) ;
  • Kang, Keum-Jee (Dept. of Food & Nutrition, DukSung Women's University)
  • 이세나 (덕성여자대학교 자연과학대학 식품영양학과) ;
  • 강금지 (덕성여자대학교 자연과학대학 식품영양학과)
  • Received : 2009.11.11
  • Accepted : 2009.12.29
  • Published : 2010.02.28
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Abstract

This study was conducted to investigate the effects of blueberry extract on cell death, ROS and gene expression patterns associated with the anti-cancer activity in human breast cancer MCF7 cells. To accomplish this, 20 mg/mL concentration of blueberry extract was added to the cell culture for 0, 6, 12, 24 or 48 h, after which the effects were evaluated by various analyses. MTT assay showed that the cellular activities decreased rapidly during the first 12 h of treatment. During this period, dual staining with Hoechst33322 and propidium iodide also produced a similar trend in which the dead or dying cells increased sharply. Furthermore, evaluation of BrdU incorporation as an index for cell proliferation revealed a marked decrease during the first 12 h of treatment, suggesting that anticancer activity involves the inhibition of cell proliferation and induces cell death. ROS also increased according to the duration of the treatment, indicating intracellular accumulation is associated with the cell death. RT-PCR analysis revealed significant decreases in anti-apoptotic (Bax) and increases in pro-apoptotic gene expressions (Bci-2, caspase- 3, and 9) (p<0.05). Taken these together, blueberry extract induces ROS accumulation in MCF7 cells, causing inhibition of cell proliferation and eventually leading to cell death. This cell death was associated with apoptotic gene expression in blueberry-treated cells for up to 24 h.

Keywords

References

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