Biomolecules & Therapeutics

The Korean Society of Applied Pharmacology (한국응용약물학회)
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A Pattern Recognition Receptor, SIGN-R1, Mediates ROS Generation against Polysaccharide Dextran, Resulting in Increase of Peroxiredoxin-1 and Its Interaction to SIGN-R1

  • Choi, Heong-Jwa (Department of Biomedical Science & Technology, Institute of Biomedical Science & Technology, Konkuk University) ;
  • Choi, Woo-Sung (Department of Biomedical Science & Technology, Institute of Biomedical Science & Technology, Konkuk University) ;
  • Park, Jin-Yeon (Department of Biomedical Science & Technology, Institute of Biomedical Science & Technology, Konkuk University) ;
  • Kang, Kyeong-Hyeon (Department of Biomedical Science & Technology, Institute of Biomedical Science & Technology, Konkuk University) ;
  • Prabagar, Miglena G. (Department of Biomedical Science & Technology, Institute of Biomedical Science & Technology, Konkuk University) ;
  • Shin, Chan-Young (Department of Pharmacology, School of Medicine, Konkuk University) ;
  • Kang, Young-Sun (Department of Biomedical Science & Technology, Institute of Biomedical Science & Technology, Konkuk University)
  • Received : 2010.06.01
  • Accepted : 2010.07.02
  • Published : 2010.07.31
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Abstract

Streptococcus pneumoniae is the major pathogen that frequently causes serious infections in children, the elderly and immunocompromised patients. S. pneumoniae is known to produce reactive oxygen species (ROS) and S. pneumoniae-produced ROS is considered to play a role in pneumococci pathogenesis. SIGN-R1 is the principal receptor of capsular polysaccharides (CPSs) of S. pneumoniae. However, there is a considerable lack of knowledge about the protective role of SIGN-R1 against S. pneumoniae-produced ROS in SIGN-$R1^+$ macrophages. While investigating the protective role of SIGN-R1 against ROS, we found that SIGN-R1 intimately bound to peroxiredoxin-1 (Prx-1), one of small antioxidant proteins in vitro and in vivo. This interaction was increased with ROS generation which was produced by stimulating SIGN-R1 with dextran, a polysaccharide ligand of SIGN-R1. Also, SIGN-R1 crosslinking with 22D1 anti-SIGN-R1 antibody increased Prx-1 in vitro or in vivo. These results suggested that SIGN-R1 stimulation with CPSs of S. pneumoniae increase the expression level of Prx-1 through ROS and its subsequent interaction to SIGN-R1, providing an important antioxidant role for the host protection against S. pneumoniae.

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