The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Anti-oxidative and anti-inflammatory effect of Do-Ki-Tang methanol extract in mouse macrophage cells

마우스 대식세포에서 도기탕 (導氣湯) 메탄올 추출물의 항산화 및 항염증 효과

  • Kim, Dong-Wan (Department of Prescriptionology, Collage of Oriental Medicine, Dongguk University) ;
  • Yun, Hyun-Jeong (Department of Prescriptionology, Collage of Oriental Medicine, Dongguk University) ;
  • Heo, Jun-Young (Department of Prescriptionology, Collage of Oriental Medicine, Dongguk University) ;
  • Kim, Tae-Hoon (Department of Prescriptionology, Collage of Oriental Medicine, Dongguk University) ;
  • Cho, Hyun-Jin (Department of Prescriptionology, Collage of Oriental Medicine, Dongguk University) ;
  • Park, Sun-Dong (Department of Prescriptionology, Collage of Oriental Medicine, Dongguk University)
  • 김동완 (동국대학교 한의과대학 방제학교실) ;
  • 윤현정 (동국대학교 한의과대학 방제학교실) ;
  • 허준영 (동국대학교 한의과대학 방제학교실) ;
  • 김태훈 (동국대학교 한의과대학 방제학교실) ;
  • 조현진 (동국대학교 한의과대학 방제학교실) ;
  • 박선동 (동국대학교 한의과대학 방제학교실)
  • Received : 2010.11.11
  • Accepted : 2010.12.15
  • Published : 2010.12.30
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Abstract

Objective : The aim of this study was to determine whether methanol extract of Do-Ki-Tang (DKT) inhibit free radical generation and production of nitrite an index of NO, $PGE_2$, iNOS, COX-2 and pro-inflammatory cytokines such as TNF-${\alpha}$, IL-$1{\beta}$, IL-6 and MCP-1 in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. Methods : Cytotoxic activity of extract on RAW 264.7 cells was measured using 5-(3-caroboxymeth-oxyphenyl)-2H-tetra-zolium inner salt (MTS) assay. The expression level of inflammatory response-related proteins was confirmed by western blot. The production of proinflammatory cytokines was measured by ELISA. Results : Our results indicated that DKT scavenged DPPH radical and nitric oxide in vitro. Moreover, DKT significantly inhibited the LPS-induced NO, $PGE_2$ production and iNOS, COX-2 expression accompanied by an attenuation of TNF-${\alpha}$, IL-$1{\beta}$, IL-6 and MCP-1 formation in macrophages. Furthermore, DKT treatment also blocked LPS-induced intracellular ROS production and the activation of NF-${\kappa}B$ and MAPKs. Conclusion : Our data suggest that the anti-inflammatory effect of DKT is mediated through down-modulation of pro-inflammatory mediators and cytokines by blocking the signaling pathways of NF-${\kappa}B$ and MAPKs. These inhibitory effects by DKT represent a potential therapeutic approach to the treatment of inflammatory diseases.

Keywords

References

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