Journal of Plant Biotechnology

  • 제37권1호
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  • Pages.96-101
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  • 2010
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  • 1229-2818(pISSN)
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  • 2384-1397(eISSN)
한국식물생명공학회 (The Korean Society of Plant Biotechnology)
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Production of glycoalkaloids from callus cultures of Solanum hainanense Hance

  • Loc, Nguyen Hoang (Institute of Resources, Environment and Biotechnology, Hue University) ;
  • Anh, Nguyen Huu Thuan (Institute of Resources, Environment and Biotechnology, Hue University) ;
  • Binh, Doan Huu Nhat (Institute of Resources, Environment and Biotechnology, Hue University) ;
  • Yang, Moon-Sik (Division of Biological Sciences and Research Center for Bioactive Materials, Chonbuk National University, Jeonju Center for Korea Basic Science Institute, Chonbuk National University) ;
  • Kim, Tae-Geum (Division of Biological Sciences and Research Center for Bioactive Materials, Chonbuk National University)
  • 투고 : 2010.01.21
  • 심사 : 2010.03.12
  • 발행 : 2010.03.31
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초록

Leaf explants of the Solanum hainanense plant, grown in vitro, were cultured in basal Murashige and Skoog (MS) media supplemented with 0.5 mg/L kinetin and 1 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) for callus initiation. For maintenance and proliferation, the callus was cultured on MS medium supplemented with 1 mg/L 6-benzylaminopurine (BAP) and 0.5 mg/L 2,4-D. The glycoalkaloid content in the callus was at its maximum after ten weeks of culture (188.65 mg/g), whereas that of the one-year-old control was 22.22 mg/g in the root and 5.99 mg/g in the stem. The glycoalkaloid extracted from the callus inhibited the activity of collagenase on collagen gel. High performance liquid chromatography (HPLC) analysis showed that biotransformation occurred when a callus was grown on medium supplemented with various carbon sources. These results suggest that callus of S. hainanense is a good material for production of glycoalkaloid.

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참고문헌

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