Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Production of glycoalkaloids from callus cultures of Solanum hainanense Hance

  • Loc, Nguyen Hoang (Institute of Resources, Environment and Biotechnology, Hue University) ;
  • Anh, Nguyen Huu Thuan (Institute of Resources, Environment and Biotechnology, Hue University) ;
  • Binh, Doan Huu Nhat (Institute of Resources, Environment and Biotechnology, Hue University) ;
  • Yang, Moon-Sik (Division of Biological Sciences and Research Center for Bioactive Materials, Chonbuk National University, Jeonju Center for Korea Basic Science Institute, Chonbuk National University) ;
  • Kim, Tae-Geum (Division of Biological Sciences and Research Center for Bioactive Materials, Chonbuk National University)
  • Received : 2010.01.21
  • Accepted : 2010.03.12
  • 발행 : 2010.03.31
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초록

Leaf explants of the Solanum hainanense plant, grown in vitro, were cultured in basal Murashige and Skoog (MS) media supplemented with 0.5 mg/L kinetin and 1 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) for callus initiation. For maintenance and proliferation, the callus was cultured on MS medium supplemented with 1 mg/L 6-benzylaminopurine (BAP) and 0.5 mg/L 2,4-D. The glycoalkaloid content in the callus was at its maximum after ten weeks of culture (188.65 mg/g), whereas that of the one-year-old control was 22.22 mg/g in the root and 5.99 mg/g in the stem. The glycoalkaloid extracted from the callus inhibited the activity of collagenase on collagen gel. High performance liquid chromatography (HPLC) analysis showed that biotransformation occurred when a callus was grown on medium supplemented with various carbon sources. These results suggest that callus of S. hainanense is a good material for production of glycoalkaloid.

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참고문헌

  1. Anh NHT, Thanh LTH, Binh DHN, Phuong TTB, Loc NH (2007) Study on the accumulation of glycoalkaloid in Solanum hainanense Hance callus. Proc the 2007th National Conference on Life Sciences. Sci and Tech Publishing House, Hanoi, Vietnam, pp. 229-232
  2. Berman MB, Manabe R, Davison PF (1973) Tissue collagenase: A simplified, semiquantitative enzyme assay. Anal Biochem 54:522-534 https://doi.org/10.1016/0003-2697(73)90384-9
  3. Chating B, Conepcion L, de Cristancho B, Usubillaga A (1997) Estudio clinico de la efectividad de extractos alcaloides optenidos de los frutos del Solanum americanum Miller sorbe el Herpes simplex, Herpes zoster y Herpes genitalis. Revista de la Facultad de Farmacia 32:18-25
  4. Friedman M, Fitch TE, Levin CE, Yokoyama WH (2000a) Feeding tomatoes to hamsters reduces their plasma low-density lipoprotein cholesterol and triglycerides. J Food Sci 65:897-900 https://doi.org/10.1111/j.1365-2621.2000.tb13608.x
  5. Friedman M, Fitch TE, Yokoyama WH (2000b) Lowering of plasma LDL cholesterol in hamsters by the tomato glycoalkaloid tomatine. Food Chem Toxicol 38:549-553 https://doi.org/10.1016/S0278-6915(00)00050-8
  6. Gubarev MI, Enioutina EY, Taylor JL, Visic DM, Daynes RA (1998) Plant-derived glycoalkaloids protect mice against lethal infection with Salmonella typhimurium. Phytother Res 12:79-88 https://doi.org/10.1002/(SICI)1099-1573(199803)12:2<79::AID-PTR192>3.0.CO;2-N
  7. Heal KG, Sheikh NA, Hollingdale MR, Morrow WJW, Taylor-Robinson AW (2001) Potentiation by a novel alkaloid glycoside adjuvant of a protective cytotoxic T cell immune response specific for a preerythrocytic malaria vaccine candidate antigen. Vaccine 19:4153-4161 https://doi.org/10.1016/S0264-410X(01)00166-9
  8. Hop VV, Phuong VX (2003) Alkaloid species belong to Solanaceae family in Vietnam. J Biol 25:27-31 (in Vietnamese)
  9. Jones A, Veliky IA (1980) Growth of plant cell suspension cultures on glycerol as a sole source of carbon and energy. Canadian J Bot 58:648-657 https://doi.org/10.1139/b80-082
  10. Komaraiah P, Ramakrishna SV, Reddanna P, Kavi Kishor PB (2003) Enhanced production of plumbagin in immobilized cells of Plumbago rosea by elicitation and in situ adsorption. J Biotechnol 101:181-187 https://doi.org/10.1016/S0168-1656(02)00338-3
  11. Macek TE (1989) Solanum aviculare Forst., Solanum laciniatum Ait: In vitro culture and the production of solasodine. In: Bajaj YPS (ed) Biotechnology in Agriculture and Forestry. Springer Verlag, Berlin Heidelberg. Vol 7, pp 443-463
  12. McGehee S, Kraswoski D, Fung L (2000) Cholinesterase inhibition by potato glycoalkaloids slows mivacurium metabolism. Anesestesiol 93:510-519 https://doi.org/10.1097/00000542-200008000-00031
  13. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473-497 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  14. Paska C, Innocenti G, Kunavari M, Laszio M, Szilagyi L (1999) Lignan production by Ipomoea batatas callus cultures. Phytochem 52:879-883 https://doi.org/10.1016/S0031-9422(99)00304-0
  15. Rao RB, Kumar DV, Amrutha RN, Jalaja N, Vaidyanath K, Rao AM, Rao S, Polavarapu R, Kishor PBK (2008) Effect of growth regulators, carbon source and cell aggregate size on berberine production from cell cultures of Tinospora cordifolia Miers. Curr Trends Biotechnol Pharm 2:269-276
  16. Subroto MA, Tampubolon E, Simanjuntak P (2007) Changes in solasodine accumulation in regenerated plants of Solanum nigrum transformed with Agrobacterium rhizogenes 15834. Biotechnol 6:328-333 https://doi.org/10.3923/biotech.2007.328.333
  17. Stanker LH, Kampos-Holtzapple C, Beier RC, Levin CE, Friedman M (1996) Detection and quantification of glycoalkaloidscomparison of enzyme-linked immunosorbent assay and high-performance liquid chromatography methods. ACS Symp Ser 621:243-251 https://doi.org/10.1021/bk-1996-0621.ch018
  18. Thu NB, Khai NM, Man PK, Nhu DT (2000) Effects of Solanum hainanense Hance on collagenase. J Pharm Materials 5:149-152 (in Vietnamese)
  19. Thu NB, Man PK (2000) Quantitative determination of glycoalkaloids in Solanum hainanense Hance by acid dye colorimetric method. J Pharm Materials 5:104-108 (in Vietnamese)
  20. Yogananth N, Bhakyaraj R, Chanthuru A, Parvathi S, Palanivel S (2009) Comparative analysis of solasodine from in vitro and in vivo cultures of Solanum nigrum Linn. Kathmandu Univ J Sci Engin Technol 5:99-103