Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Expression and regulation of self-incompatible genes in Brassica

배추과 작물의 자가불화합성 유전자의 발현 및 조절

  • Park, Jong-In (Department of Horticulture, Sunchon National University) ;
  • Lee, In-Ho (Department of Horticulture, Sunchon National University) ;
  • Watanabe, Masao (Graduate School of Life Sciences, Tohoku University) ;
  • Nou, Ill-Sup (Department of Horticulture, Sunchon National University)
  • 박종인 (순천대학교 생명산업과학대학 원예학과) ;
  • 이인호 (순천대학교 생명산업과학대학 원예학과) ;
  • ;
  • 노일섭 (순천대학교 생명산업과학대학 원예학과)
  • Received : 2010.03.31
  • Accepted : 2010.04.10
  • Published : 2010.06.30
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Abstract

In most self-incompatible plant species, recognition of self-pollen is controlled by a single locus, termed the S-locus. The self-incompatibility (SI) system in Brassica is controlled sporophytically by multiple alleles at a single locus, designated as S, and involves cell-cell communication between male and female. Two highly polymorphic S locus genes, SLG (S locus glycoprotein) and SRK (S receptor kinase), have been identified, both of which are expressed predominantly in the stigmatic papillar cell. Gain-of-function experiments have demonstrated that SRK solely determines S haplotype-specificity of the stigma, while SLG enhances the recognition reaction of SI. The sequence analysis of the S locus genomic region of B. campestris (syn. rapa) has led to the identification of an anther-specific gene, designated as SP11/SCR, which is the male S determinant. Molecular analysis has demonstrated that the dominance relationships between S alleles in the stigma were determined by SRK itself, but not by the relative expression level. In contrast, the expression of SP11/SCR from the recessive S allele was specifically suppressed in the S heterozygote, suggesting that the dominance relationships in pollen were determined by the expression level of SP11/SCR. Furthermore, recent studies on recessive allele-specific DNA methylation of Brassica self-incompatibility alleles demonstrate that DNA methylation patterns in plants can vary temporally and spatially in each generation. In this review, we firstly present overview of self incompatibility system in Brassica and then describe dominance relationships in Brassica self- incompatibility regulated by allele-specific DNA methylation.

Keywords

References

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